Description

Designation:NRS1221A
DepositedName:Bacillussubtilisvar.nigerSmithetal.
ProductSheet ProductDescription:Usedasasterilizationcontrolstrain.ThisstrainisrecommendedbyATCCforusein
thetestsdescribedinmilitaryspecificationMILS36586AandU.S.Pharmacopeia,23rdrev.,<1211>,pp.
Bacillusatrophaeus 19761981,1995whereonlythetaxonisspecified.Usedinsterilityassurance,asadryheatsterilization

(ATCC9372) control,andethyleneoxidesterilizationcontrol.

Propagation

PleasereadthisFIRST
Medium
ATCCMedium3:Nutrientagarornutrientbroth
StorageTemp.
Frozen:80Cor GrowthConditions
Temperature:30C
colder
Atmosphere:Aerobic

FreezeDried:2C
to8C PropagationProcedure
LiveCulture:See 1. Openvialaccordingtoenclosedinstructions.
2. Usingasingletubeof#3broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
Propagation
1.0mLpipette.Rehydratetheentirepellet.
Section
3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.
4. Useseveraldropsofthesuspensiontoinoculatea#3agarslantand/orplate.
5. Incubatethetubesandplateat30Cfor24hours.
BiosafetyLevel

1
Notes

FormerlyBacillussubtilis.DepositedasBacillussubtilisvar.niger.
IntendedUse
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

Thisproductisintendedforresearchuseonly.Itisnot
References
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bacillusatrophaeus(ATCC9372)
BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein
mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
longervalid.

Disclaimers

AmericanTypeCultureCollection Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
POBox1549 WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
Manassas,VA20108USA ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
www.atcc.org
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
800.638.6597or703.365.2700
Fax:703.365.2750 Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
Email:Tech@atcc.org isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
Orcontactyourlocaldistributor
themisidentificationormisrepresentationofsuchmaterials.

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 PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2016.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[01/27]

ProductSheet
Bacillusatrophaeus
(ATCC9372)

PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder

FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain

Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bacillusatrophaeus(ATCC9372)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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