Mansi Manocha

Mobile: 416-574-6792 Email: mansi.manocha@gmail.com Canadian Permanent Resident

Education ________________________________________________________
Masters in Research (MRes) in Advanced Genomic and Proteomic Sciences, University of
Nottingham, U.K, Sept 2008 Oct 2009(1 year degree); Marks: 60% (Merit)
(equivalent to a Masters degree as determined by World Education Services (WES), Toronto,
ON)
Project: Effects of nutritional and pharmacological interventions on zonal phenotype of HepG2
cell-lines to understand the role of these interventions in phenotypic transition in human liver.

Bachelors of Technology (B. Tech) in Biotechnology, Jaypee Institute of Information

Technology University (JIITU), India, June 2004 May 2008 (4 year degree); Marks: CGPA
of 7.4 out of 10 (First Class)
(equivalent to a Bachelors degree (4 years) as determined by WES, Toronto, ON)
Project: Characterization, cloning and expression of a novel cDNA isolated by screening
chicken heart cDNA library.

Research Experience__________________________________________
Research Intern, Department of Biochemistry, All India Institute of Medical Sciences
(AIIMS), India, April 2017- June 2017 (2 months)
Project: Effect of cortisol analogue Dexamethasone (Dex) on breast cancer in terms of cancer
cell proliferation, apoptosis and survival.

Research Scientist, Institute of Liver Transplantation and Regenerative Medicine,

Medanta Hospital, India, Jan 2012 April 2016 (4 years 4 months)
Grant writing, Planning and lab work, Clinical trial documentation and Patient interaction,
Data collection and analysis using MS excel and healthcare information system (eHIS)
Main project: Intra-peritoneal transplantation of encapsulated hepatocytes for acute liver
failure; Pilot project: Isolation and cryopreservation of human hepatocytes.
Specific Techniques: Liver tissue digestion and purification of hepatocytes,
Cryopreservation of hepatocytes, Cell count and viability assessment (trypan blue
exclusion) and Hepatocyte specific functional assays.
In pipeline: Molecular and genetic profiling of hepatocellular carcinoma towards better
diagnosis and prognostication; Molecular marker profiling after loss of liver volume in living
donor liver transplantation.
Training: ICH Good Clinical Practice for Clinical Trial Sites, Quintiles, U.S.A and well-
versed with schedule Y and ethical guidelines for biomedical research

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Summer Intern, Ranbaxy Laboratories, India, May 2007 - July 2007 (6 weeks)
Project: Cloning and expression of the gene of DNA gyrase from quinolone-resistant
Mycobacterium tuberculosis

Summer Intern, National Bureau of Plant Genetic Resources, India, June 2006 July 2006
(6 weeks)
Project: Application of molecular markers to determine the genetic diversity in Medeola
virginiana L. germplasms.
Specific Techniques: Random Amplified Polymorphic DNA (RAPD) and Sequence-
tagged microsatellites (STMs) amplification by PCR; genomic plant DNA isolation,
purification and quantification.

Trainee, Indian Agricultural Research Institute, India, Dec 2004 Jan 2004 (2 months)
Project: Invitro Regeneration of Solanum lycopersicum using Plant Tissue Culture Techniques.

Lab skills__________________________________________
Cell and tissue culture techniques: Breast cancer cell line culture (MDA-MB-231 and MCF-
7), Liver cancer cell line culture (HepG2), Plant Tissue Culture.
Molecular biology techniques
Biochemical assays, Whole cell lysate preparation, Nuclear and cytoplasmic protein
extraction, Western blotting, Fast Protein Liquid Chromatography (FPLC)
RNA isolation, Agarose gel electrophoresis, Microarray and its analysis using
Genespring software
Competent cell preparation, Plasmid transformation, Plasmid preparation, Restriction
digestion, Gel extraction, DNA ligation
IPTG Induction and Extraction of Proteins, SDS- PAGE
Bioinformatics tools like Expressed Sequence Tags database search, BLAST and BLAT
cDNA synthesis, Conventional PCR and Real- time PCR, Flow cytometric cell cycle analysis
by DNA content (Fixation of cells and Propidium iodide DNA staining)

Health and safety guidelines: ACDP, ACGM and COSHH

Professional Experience__________________________________________
Medical Administrator, Hurley Clinic NHS clinic, U.K, Jan 2010 Feb 2011 (1 year
2 months)
Summarized medical records using healthcare information system
Analysed data for the Smoking Cessation programme; Streamlined in a team of three, 600
phone calls and 250 patients a day; Infection Control training, Feb 2011.

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Scientific Presentations___________________________________
Effects of nutritional and pharmacological interventions on zonal phenotype of HepG2
cell-lines., Oct 2009, University of Nottingham, U.K.
Characterisation, cloning and expression of a novel cDNA isolated by screening chicken
heart cDNA library., May 2008, JIITU, India

Conference Posters and Awards______________________________________

Poster presentation at 19th International Congress of the International Liver Transplant
Society (ILTS) June 2013, Australia. Abstract published: Hepatocyte isolation and
cryopreservation: Yield from four different sources [P-219] Liver Transplantation 2013 June:
19(S1); S201
International scholarship: University of Nottinghams Developing Solutions Scholarship
Programme 2008-09

Exam scores______________________________________
TOEFL iBT: 113/120
GRE 2017: Quantitative section: 158/170; Verbal section: 155/170; Analytical writing:
3.5/6

References______________________________________
Available upon request

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