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ABSTRACT
The Robusta coffee flowers were analyzed for phytochemical composition and
antioxidant activity. Flower powders at three different stages were extracted successively with
hexane, dichloromethane and methanol, resulting in the extracts of varying yields (1.61-
16.01%). All crude extracts were constituted of flavonoids, terpenoids and alkaloids. The
methanol crude extracts exhibited the best antioxidant activity on both DPPH (EC 50 0.03-0.13
mg/mL) and reducing power (EC50 0.19-0.46 mg/mL). It also contained the highest amount of
total phenols (28.32-139.88 mg GAE/g extract).
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Vol.22, No.1, January-March 2017 Thammasat International Journal of Science and Technology
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Thammasat International Journal of Science and Technology Vol.22, No.1, January-March 2017
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DPPH + ++ +++
Flavonoids
Sofowora + + +++
Table 2. Total phenols, IC50 values of DPPH and reducing power activity of extracts from
Robusta coffee blossoms.
Stages of Crude extracts/ Total phenol content
Blossom Synthetic EC50 (mg/mL)
(mg GAE/g crude
antioxidants extract) DPPH Reducing power
Hexane 6.43 0.71 5.56
Dichloromethane 25.96 0.30 1.75
1
Methanol 28.32 0.13 0.46
(overall) (60.71)
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Vol.22, No.1, January-March 2017 Thammasat International Journal of Science and Technology
5. Acknowledgement
We would like to thank Division of
Chemistry, Department of Science, Faculty
of Science and Technology, Prince of
Songkla University, Pattani Campus,
Thailand, for support with chemicals and
equipment.
6. References
[1] Dagoon J. Agriculture & Fishery
Technology IV. Manila: Rex
Bookstore, 2005.
[2] Damatta FM, Ronchi CP, Maestri M,
Barros RS. Ecophysiology of coffee
growth and production. Braz J Plant
Physiol 2007;19(4):485-510.
[3] Coffee: World Markets and Trade
2016. Foreign Agricultural Service,
[cited 2016 July 20]. Available from:
http://www.fas.usda.gov/data/coffee-
world-markets-and-trade
[4] Office of Agricultural Economics:
Agricultural statistics of Thailand
2015 [Internet]. [cited 2015
September 17]. Available from:
Fig. 3. Absorbance on reducing power of http://www.oae.go.th/download/down
crude extracts from Robusta coffee blossom load_journal/2559/yearbook58.pdf
(a) Hexane (b) Dichloromethane (c) [5] Pohlan HAJ, Janssen MJJ. Growth and
Methanol. production of coffee in soils, plant
growth and crop production. Oxford:
EOLSS Publishers; 2010.
4. Conclusion [6] Yashin A, Yashin Y, Wang JY,
This study provided a brief Nemzer B. Antioxidant and antiradical
investigation of phytochemical constituents activity of coffee. Antioxidants 2013;
of Robusta coffee flowers. The hexane, 2:230-45.
dichloromethane and methanol crude [7] Vignoli JA, Viegas MC, Bassoli DG,
extracts contained terpenoids, flavonoids and Benassi MT. Roasting process affects
alkaloids in different amounts. Their differently the bioactive compounds
antioxidant properties were also proved to be and the antioxidant activity of Arabica
effective. The antioxidant activity and total
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and Robusta coffee. Food Res Int [17] Naidu MM, Sulochanamma G,
2014;61:279-85. Sampathu SR, Srinivas P. Studies on
[8] Hernandez LMP, Quiroz KC, Juarez extraction and antioxidant potential of
LAM, Meza NG. Phenolic green coffee. Food Chem 2008;
characterization, melanoidins, and 107(1):377-384.
antioxidant activity of some comm
ercial coffees from Coffea Arabica and
Coffea canephora. J Mex Chem Soc
2012; 56(4):430-5.
[9] Tsao R, Deng Z. Separation
procedures for naturally occurring anti
oxidant phytochemicals. J Chromatogr
B 2004;812:85-99.
[10] Tiwari P, Kumar B, Kaur M, Kaur G,
Kaur H. Phytochemical screening and
extraction: A review. IPS 2011;1(1):
98-106.
[11] Ashfaq M, Shah KW, Ahmad S, Singh
D. Preliminary phytochemical screen
ing of alcoholic & aqueous extracts of
Mentha Longifolia Linn. Leaves. Int J
Pharm Biol Sci 2012;3(3) 384-6.
[12] Singleton VL, Rossi J. Calorimetry of
total phenolic with phosphomolybdic-
phosphotungstic acid agents. Am J
Enol Vitic 1965;16:144-158.
[13] Shimada K, Fujikawa K, Yahara K,
Nakamura T. Antioxidative properties
of xanthan on the autooxidation of
soybean oil in cyclodextrin emulsion.
J Agric Food Chem 1992;40:945-8.
[14] Oyaizu M. Studies on products of
browning reaction: Antioxidative
activities of products of browning
reaction prepared from glucosamine.
Jpn J Nutr 1986;44:307-315.
[15] Chairgulprasert V, Prasertsongskun S,
Junpra-ob S, Sanjun M. Chemical
constituents of the essential oil,
antioxidant and antibacterial activities
from Elettariopsis curtisii Baker.
Songklanakarin J Sci Technol
2008;30(5):591-6.
[16] Brand-Williams W, Cuvelier ME,
Berset C. Use of a free radical method
to evaluate antioxidant activity.
Lebensm Wiss Technol 1995;28:25-
30.