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User Manual
Manufacturer Information
Safety regulations 5
Get Ready! 19
Get Set! 26
Go!35
Dimensions 40
The individual modules of the Leica DM micros- Before installing, operating or using the instru-
copy series include an interactive CD-ROM with ments, read the user manuals listed above. In
all relevant user manuals several languages. particular, please observe all safety instructions.
Keep it in a safe place, and readily accessible
to the user. User manuals and updates are also To maintain the unit in its original condition
available for you to download and print from and to ensure safe operation, the user must
our website at www.leica-microsystems.com. follow the instructions and warnings contained
in these user manuals.
This operating manual describes the special
functions of the individual modules of the
Leica DM microscopy series and contains
important instructions for their operational
safety, maintenance, and accessories.
This symbol indicates additional infor-
OO Functional disturbances or damaged mation or explanations that intend to
instruments provide clarity.
This symbol indicates especially impor- ff This symbol within the text points to addi-
tant information that is mandatory to tional information and explanations.
read and observe.
EC Declaration of Conformity
Electrically operated accessories are
constructed based on the state of the art of
technology and are provided with an EC Decla-
ration of Conformity.
Never install any other plug or unscrew
any mechanical components unless
the devices from oil, chemicals and extreme expressly instructed to do so in the instructions.
The Leica DM500 microscope can be used in humidity. If using the devices outdoors, protect
them from dust and moisture. Never use electri-
this instruction manual have been tested
clean rooms without any problems. The devices and accessories described in
cal devices outdoors. Install electrical devices at
Always position the Leica DM500 micro- least 10 cm from the wall and away from flam- for safety and potential hazards.
scope so that you can disconnect it from mable substances.
the power supply at any time. The power cable The responsible Leica affiliate must be
must remain accessible at all times, because it is Avoid large temperature fluctuations, direct consulted whenever the instrument is
intended as a power disconnect device. sunlight and vibrations. These conditions altered, modified or used in conjunction with
can distort measurements and micrographic non-Leica components that are outside of the
images. scope of this manual!
the
In warm and warm-damp climatic zones,
individual components require
special care in order to prevent the build-up of
Unauthorized alterations to the instru-
ment or noncompliant use shall void all
rights to any warranty claims.
fungus.
Workplaces with microscopes facilitate Direct contact with eyepieces is a OO The Leica DM500 microscope may only be
and improve the viewing task, but they potential transmission method for viral connected to a grounded socket.
also impose high demands on the eyes and infections of the eye.
holding muscles of the user. Depending on the OO The Leica DM500 microscope may not be
duration of uninterrupted work, asthenopia operated unless it is in proper functioning
The risk can be kept to a minimum by
and musculoskeletal problems may occur. For using personal eyepieces for each indi- condition.
this reason, appropriate measures for reduction vidual or detachable eyecups.
of the workload must be taken: The microscope illumination is in the exempt
group (risk group 0) according to EN 62471:2008
OO Optimum workplace layout when used according to its intended use.
OO Thorough training of the personnel, giving with or without optical instruments as this
consideration to ergonomic and organiza- increases the risk class. Failure to observe this
tional aspects notice poses a risk of eye damage.
Information for the person responsible for OO Do not use the Leica DM500 microscope OO Only original Leica replacement parts may
the instrument unless it is in perfect condition. be used in servicing the product.
OO Ensure that the Leica DM500 microscope is
used only by qualified personnel. OO Inform your Leica representative or OO After service work or technical modifica-
Leica Microsystems (Schweiz) AG, tions, the unit must be reconfigured with
OO Ensure that this user manual is always avail- 9435 Heerbrugg, Switzerland, immediately observance to our technical requirements.
able at the Leica DM500 microscope. of any product defect that could potentially
cause injury or harm. OO If the unit is modified or serviced by unau-
OO Carry out regular inspections to make thorized persons, is improperly maintained
certain that the authorized users are adher- OO If you use accessories made by third-party (as long as maintenance was not carried
ing to safety requirements. manufacturers with the Leica DM500 micro- out by us), or is handled improperly, Leica
scope, be sure that each such manufacturer will not accept any liability.
OO When instructing new users, do so thor- confirms the safety-engineering, harmless
oughly and explain the meanings of the usability of the product and observe the OO The electric installation in the building
warning signs and messages. product's user manual. must conform to the national standard, e.g.
current-operated ground leakage protec-
OO Assign individual responsibilities for start- OO Modifications and maintenance of the tion (fault-current protection) is suggested.
ing, operating and servicing the instru- Leica DM500 microscope may only be
ment and monitor the observance of these performed by professionals expressly
responsibilities. authorized by Leica.
General instructions Cleaning coated parts and plastic parts Cleaning glass surfaces
OO Protect the Leica DM500 microscope OO Dust and dirt particles should be removed OO Remove dust using a dry and grease-free
against damp, vapors, acids, alkalis, and with a soft brush or lint-free cotton cloth. brush made from hair, by blowing with
corrosive substances. Do not store chemi- bellows, or by using a vacuum.
cals in the vicinity. OO Remove coarse debris with a moistened
disposable cloth. OO Optical surfaces should be cleaned with a
OO Protect the Leica DM500 microscope from lint-free cloth, lens tissue, or cotton swab
oil and grease. Never grease or oil mechani- OO Acetone, xylene or nitro-containing thin- moistened with a commercially available
cal parts or sliding surfaces. ners must NOT be used. glass cleaner.
OO Follow the instructions of the disinfectant OO Never use chemicals to clean colored
manufacturer. surfaces or accessories with rubberized
parts. This could damage the surfaces,
OO It is advisable to enter a service agreement and specimens could be contaminated by
with Leica Service. abraded particles.
Unplug the instrument before changing any fuses. The Temperature for use +10 C +40 C
Leica DM500 has two fuses, which are located behind the Storage temperature -20 C +52 C
power cord receptacle.
+50 F +104 F
Manipulation shock 25 mm on 50 mm hard wood
Type 1: Leica EZ viewing tube with integrated 2. Insert the dovetail into the stand support
eyepieces and tighten the set screw firmly without
forcing it. This precisely positions the view-
ing tube onto the optical axis of the micro-
scope, regardless of the selected rotation.
1. To use the captive thumbscrew, completely 2. Position the Leica EZ viewing tube back in
remove the set screw that comes with the place on the stand.
stand.
or replace the set screw with the (optional) 3. Attach the captive thumbscrew using the
captive thumbscrew. Be sure the thumbscrew portion of the wrench tool that comes with the captive
captive thumbscrew is fully loosened thumbscrew.
before attaching it to the stand:
1. Insert the eyepieces into the eyetubes. 2. Lock the eyepieces into the eyetubes by
tightening the silver screws on the under-
side of the eyetubes with a standard Cross
type (Phillips) screwdriver (not provided).
able dovetail.
The standard viewing tube has a rotat-
Therefore, you can now
rotate the standard viewing tube freely in any
orientation.
viewing, keep the rubber eyeguards scope configuration, you will notice that
If you wear eyeglasses for microscope If you have purchased a standard micro-
folded down. If you do not wear eyeglasses, the objectives are already installed on the nose-
you may find it useful to unfold the rubber piece, and the substage condenser is already
eyeguards in order to help block out ambient installed on the stand. In this case, go to section
room light. "Operation" on page 27. If you purchased your
Leica DM500 by components and not by stan-
dard configuration, please continue to section
"Installing Objectives" on page 25.
Always rotate the nosepiece by using The Leica DM500s substage condenser
the knurled nosepiece ring. is pre-installed, pre-focused, and pre-
centered. No additional installation or align-
1. As you rotate the nosepiece clockwise, ment is required.
attach the objectives by screwing them
into the nosepiece holes starting with the
lowest magnification and advancing to the
highest magnification.
The Leica DM500 has a 5 V/1.5 A USB power Set the illumination control knob
base. Never use an adapter between the power connector in the center of the cord wrap. This (located on the bottom left of the
cord and the power source; it will render the can be used to power some Leica cameras or microscope stand) to the lowest setting to
grounding feature ineffective. other devices which require 5 V/1.5 A. start. The illumination control knob allows you
to adjust the intensity of light produced by the
illumination system.
Work surface
Power cord
Plug in and turn on the microscope Using the condenser 2. To start, fully open the iris diaphragm of
1. Plug the microscope power cord into a suit- The condenser is furnished with an iris the aperture condenser by rotating the
able grounded electrical outlet. diaphragm, which can be adjusted to condenser ring to the extreme right.
match the effective numerical aperture of each
objective.
A grounded 3-wire cord is provided.
1. To open and close the iris diaphragm,
simply rotate the knurled condenser ring to
2. Turn the microscope on using the switch on the right or to the left so that the line on
the bottom right-hand side of the micro- the rotating ring aligns with the objective
scope stand. magnification in use.
Prepare to view a specimen slide 2. Use the X/Y stage control to position the
1. Place a specimen slide on the stage by slid- slide so a portion of the specimen is under
ing the specimen slide forward into the the objective in use.
slide grips.
Focusing
1. Rotate the nosepiece (using the knurled 2. Raise the stage by rotating the coarse 3. Look into the eyepieces and adjust the illu-
nosepiece ring) to move the lowest magni- adjustment knob to its positive stop at the mination intensity so that it is comfortable
fication objective into working position. highest position. for viewing.
1. Adjust the eyetubes for your interpupillary Leica EZ viewing tube Standard tube with one or two focusing
distance. Fold or unfold the eyetubes to If you are using a Leica EZ viewing tube, which eyepieces
decrease or increase the distance between already has the eyepieces integrated with If you are using a standard viewing tube with
the eyepieces until you see one illuminated the eyetubes, there are no additional adjust- one or two focusing eyepieces, you need to
circle. ments necessary. Be sure you are wearing your make some adjustments:
eyeglasses or contact lenses. Go on to section
"Oil Immersion" on page 33. 1. Set the focusing eyepieces to the "0"
diopter setting.
corrective
If you are comfortable wearing your 3. Now switch to look only through the other 5. Now switch to a high magnification objec-
lenses (contact lenses or eyepiece (focusing eyepiece) with your tive (not an oil objective) and focus the
eyeglasses) for microscope viewing, leave them other eye. This time, focus the specimen by microscope while viewing with both eyes.
on and your adjustments will be minimal. using the focusing capability in the focus-
adjustment knob of the microscope while Do not raise or lower the stage to focus. focusing with a high magnification, you will
looking only through one of the eyepieces find that when you change to lower magnifi-
(if you have one focusable and one non- cations, you only have to adjust the fine focus
focusable eyepiece, look through the non- slightly, if at all.
focusable eyepiece at this time). To help 4. Grip the knurl on the focusing eyepiece
focus, cover or close the other eye. with one hand and rotate the top of the
eyepiece with the other hand until the
specimen is in focus with this eye and this
focusing eyepiece. This corrects for any
vision differences between your right eye
and left eye.
Oil immersion technique 3. Place a drop of Leica Immersion Oil on the 5. Raise the stage slowly using the coarse
1. Find the field of view on the slide that you specimen slide on the area of the specimen focus knob until the drop of oil on the spec-
would like to inspect. that you were inspecting. imen slide just comes in contact with the
front of the oil immersion objective lens.
2. Lower the stage to its lowest position using
the coarse focus knob.
General OO The cord wrap allows you to wrap the cord OO If any optical surface becomes coated with
Always carry the microscope using two in such a way that only the length you need dust or dirt, clean the surface by blowing it
hands. There is a handle on the back of is extended. off with a syringe or brushing it off with a
the microscope and an undercut in the front for camel hair brush before attempting to wipe
this purpose. the surface clean.
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