Maintenance Guide

HA-22/20/VET Hematology Analyzer

CLINDIAG SYSTEMS B.V.B.A.

 Contents
1 CIRCUIT.........................................................................................................1
1.1 SWITCH......................................................................................................1
1.2 TRANSFORMER...........................................................................................1
1.3 MOTHER BOARD........................................................................................2
1.3.1 WBC and RBC/PLT Signal Unit........................................................3
1.3.2 HGB Circuit........................................................................................4
1.3.3 Negative Pressure and Liquid Level Circuit......................................5
1.3.4 A/D Transformation............................................................................6
1.3.5 Power Supply....................................................................................7
1.3.6 Central Processing Unit.....................................................................8
1.3.7 Tubing Drive Circuit...........................................................................8
1.4 VOLUME CONTROL AND ALARM..................................................................9
1.5 RECORDER..............................................................................................10
2 TUBING SYSTEM..............................................................................................11
2.1 TUBING FUNCTIONS..................................................................................11
2.2 TUBING STRUCTURE.................................................................................11
2.2.1 Valves..............................................................................................11
2.2.2 Stepping Motor................................................................................13
2.2.3 WBC and RBC Cup.........................................................................14
2.2.4 Sampling Unit..................................................................................20
2.2.5 Diluent and Lyse Adding Unit..........................................................21
2.2.6 Vacuum Generation Unit.................................................................22
2.2.7 Problem and Solution......................................................................23
2.3 WORK CHART..........................................................................................23
2.3.1 Startup.............................................................................................23
2.3.2 Shut Down.......................................................................................24
2.3.3 Priming............................................................................................24
2.3.4 Counting..........................................................................................25
2.3.5 Lyse Adding.....................................................................................25
2.3.6 Diluent Adding.................................................................................26
2.3.7 Sampling and Discharging..............................................................26
2.3.8 Inner Cleaning of Sampling Needle................................................27
2.3.9 Outer Cleaning of Sampling Needle................................................27
2.3.10 Sample Mixing...............................................................................28
2.3.11 Declog............................................................................................28
2.3.12 WBC Counting...............................................................................28
2.3.13 RBC Counting................................................................................28
2.3.14 Measurement Unit Cleaning..........................................................29
2.3.15 Vacuum Chamber Drainage..........................................................29
3 ADJUSTMENT.............................................................................................29
3.1 HARDWARE ADJUSTMENT.........................................................................29
3.1.1 Vacuum Pressure............................................................................29
 3.1.2 HGB Voltage....................................................................................30
3.1.3 Voltage Plus.....................................................................................30
3.2 SOFTWARE TEST.....................................................................................32
3.2.1 Self Test...........................................................................................32
3.2.2 Motor Adjustment............................................................................32
4 FINAL TEST.................................................................................................33
4.1 STARTUP.................................................................................................33
4.2 DECLOG AND PRIME.................................................................................34
4.3 BACKGROUND TEST.................................................................................34
4.4 CALIBRATION............................................................................................34
4.5 PRINTER TEST.........................................................................................34
5 SPARE PARTS LIST....................................................................................35
6 APPENDIX....................................................................................................36
6.1 COUNTING TIME.......................................................................................36
6.2 SPECIFICATION ON MAINTENANCE............................................................36
6.3 Electric Diagram.....................................................................................37
1 Circuit

1.1 Switch

Figure 1-1 Switch

Power input: AC 220V/110V
Power output: 12V or 24V
Main components:
-- Input circuit
-- Transformer
-- Control circuit
-- Protective circuit

Problem and Solution

-- Problem: switch on the power but no power input.
Solution: check if the fuse is broken and replaced the fuse. If the fuse is
normal, please replace the switch.

1.2 Transformer

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 Figure 1-2 Transformer
Power input: AC 180V AC 250V.
Power output: AC38V, AC13V or AC120V.
Model: EI
Please refer to Figure 1-3.

AC 33V

AC 220V

AC 13V

AC 13V

Figure 1-3 Power transformation

Problem and Solution

-- Problem: all test results are 0.
Solution: Use a voltage meter to check out the current flowing through the
magnification circuit. If no such current can be tested, it is very possible that
the secondary coil may be broken. The solution can be replacement of the
transformer.

1.3 Mother Board

Its main functions are as follows:
-- WBC and RBC/PLT signal front amplification
-- HGB signal measurement
-- Negative pressure measurement
-- Liquid level measurement

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-- AD transformation
-- Power supply
-- Central processing unit
-- Input and output
-- Tubing drive circuit

Figure 1-4 Mother board

1.3.1 WBC and RBC/PLT Signal Unit

Structure
-- The analog single enters circuit magnification unit through BNC connector.
-- The power of front circuit magnification is A 12v, A -12v and constant
current power A 33v.
-- Left side is RBC circuit unit and the right side is WBC circuit unit.

Test pulse signal Signal magnifier Signal filter

Test pulse signal Signal buffer

Working principle
The signal magnification unit has channels, namely WBC channel and
RBC/PLT channel.
WBC channel: under the constant source flow, a certain value of pulse
voltage is produced when the WBC pass through the micropore. The voltage
is first magnified by OP37 and then TL072. User can adjust the VR9 to add
magnification rate.
RBC/PLT channel: under the constant source flow, a certain value of pulse
voltage is produced when the WBC pass through the micropore. The voltage
is first magnified by OP37 and then TL072. User can adjust the VR7 to add
magnification rate.
Problem and Solution

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 -- Problem: Result of WBC is 0.
Solution: If the background test result is also 0, it is possible that the chip of
OP37 is broken. Replace the chip and the problem can be solved.
-- Problem: WBC result is abnormal (high LY% value).
Solution: Multiple possible reasons including ambient temperature, lyse,
probe leakage, voltage plus etc. Adjust the WBC voltage plus may solve this
problem.
-- Problem: PLT result is high.
Solution: The voltage plus is abnormal. Background test result is high. The
most possible reason is disturbance. Check the ground connection and keep
the instrument a distance from high power devices.
-- Problem: WBC histogram is compressed.
Solution: Check the voltage plus around the front of mother board and the
lyse quantity.

1.3.2 HGB Circuit

Structure
-- HGB analog signal is connected with the signal test unit of WBC cup.
-- The A 12v is transformed into 5v through module 317 providing current for
HGB signal test unit.

HGB analog Signal Voltage Magnifier

A/D Transmission Voltage Magnifier

Working Principle
The HGB circuit receives and controls HGB signal from WBC cup. The signal
is transformed and magnified and then sent to A/D unit in CPU.
A certain current is provided for LBD under the control of VR3.

Problem and Solution

-- Problem: HGB bubbles.
Solution: Under the page of Main Menu, select 7 System Set and press Ok.
Press and Ok, the system will ask for password. The default password is
3333. Then the display is shown as Figure 1-5.

1. HGB without lamp 6. Amount Board Test

2. HGB Blank 7. Data Factor

3. Vacuum Pressure 8. Graphic Debug

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 4. Valve Test 9. WBC Current Off

5. Error Amount 10. RBC Current Off

Figure 1-5
a. HGB without lamp.
Select the column and press Ok. The value displayed should be lower than
0.003.
b. HGB Blank.
Select the column and press Ok. The value displayed should be 2.60.05.
If the above two values are out of range, adjust VR1 and VR3 to acquire the
right value.
-- Problem: HGB value is 0.
Solution: check out the LBD. If the light is not working, check the 317 audion.
Check out all HGB connections.

1.3.3 Negative Pressure and Liquid Level Circuit

Structure
-- Negative Pressure Circuit.

Pressure Signal Differential Amplifier Comparison Amplifier

Output Signal Isolation

-- Liquid Level and Reagent Circuit

Signal Tested Signal Magnifier CPU

Working Principle
-- Negative pressure circuit: Signal is magnified through circuit. The pressure
sensor detects value in the vacuum chamber. The pressure signal is
magnified through differential magnifier and comparison magnifier and then
output through opt-iso.
-- Liquid level circuit: the circuit consists of lyse unit, detergent unit, diluent
unit and waste unit. In the circuit the signal is magnified and output and then
processed through CPLD.
-- Reagent Test Circuit: In the circuit the liquid level signal is processed

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through CPLD and sent to CPU.

Problem and Solution

-- Problem: low vacuum rate (alarm).
Solution: check the vacuum pump. If the pump is working, check negative
pressure circuit. If there is no voltage output, probably there is a broken
capacitance in the board. Replace the capacitance.
-- Problem: Waste container is full (Alarm).
Solution: Use a voltage meter to check out if there is short circuit in the waste
sensor. Rectify the poles and the problem can be solved.

1.3.4 A/D Transformation

Analog Signal A/D Switch FIFO Buffer CPU

5V Input
Working Principle
The analog signal of WBC, RBC and PLT is respectively transformed by 3
A/D chips. The acquired data is directly sent to FIFO after which CPU reads
related value from FIFO.
HGB analog signal is processed through an A/D transformer inside PSP.

1.3.5 Power Supply

Structure

Figure 1-6

Working Principle
The power for mother board has 2 modes, namely analog power and digital

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power. The analog power is provided by transformer and the digital power is
provided by switch. The switch provides 5v and 24 v voltage for digital circuit.
The 5v is the power for circuit of CPU, FIFO, recorder, printer, memory, timer,
reagent test, display connector and keyboard.
The 12v is transformed into 3.3v for CPU.
The 24v is the power for value, pump and motors.
AC 38v is transformed into 35v for magnification circuit of WBC, RBC and
PLT.
The AC 28v is transformed into AC -12v and AC12v.
The A -18v and A 18v provides power for the magnification circuit of HGB.

Problem and Solution

-- Problem: no power input.
Solution: Check the power modules.
-- Problem: Display is lighting but no any content.
Solution: CPU is not working or no 3.3v input or voltage regulation module is
broken (7812). Replace the module or reconnect related connectors can solve
the problem.

1.3.6 Central Processing Unit

Structure

Real Time Control Restart

Flash Memory Recorder Drive

CPU
Outer Printer Drive FIFO

RS232 Connector CPLD

Problem and Solution

-- Problem: the default interface cant be reached.
Solution: Probably the CPU is not working normally. Replace the CPU.
-- Problem: System sends alarm on motor.
Solution: Chip controlling the motor is broken or connection is not well.
Check the chip and perform amendment.

1.3.7 Tubing Drive Circuit

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 Structure

Control Circuit Drive Circuit Isolation Circuit

Valve Switch Pump Motor

Working Principle
The tubing drive unit consists of control circuit, CPLD and isolation circuit and
drive circuit.

Problem and Solution

-- Problem: motor is not working or steps of motor are not regular.
Solution: MOS tube or LBD is broken. Replace the broken components.

1.4 Volume Control and Alarm

Figure 1-7 Volume control board

Function
The volume control board is used to fix volume for test. It consists of:
-- Opto switch: control volume absorbed.
-- Glass tube: two tubes are fixed on the board. Volume is 0.2ml.
-- Control circuit: compare and magnify signals.

Figure 1-8 Alarm board

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 The alarm board shown as Figure 1-8 is used to detect diluent and lyse left.
If no liquid left, the system will send alarm.

Problem and Solution

-- Problem: WBC bubbles (alarm).
Solution: Check the run time of liquid inside WBC tube. If the time is longer
than set time, it may lead to bubbles. Check valve9 and valve10 when
counting.
-- Problem: WBC clog (alarm).
Solution: Pipette connector with the WBC glass tube is loose. Rectify the
connection.
-- Problem: Count time is 0.
Solution: MTB optical is dirty.
-- Problem: Alarm on WBC or RBC clog can be read out on screen, but test
results are normal.
Solution: Check the volume board. Replace it if it is not working normally.

1.5 Recorder

Figure 1-9
Structure

Data control wire FIFO Paper test

Heat
CPU
sensor
Protector Stepping

Temperature test

Working Principle
The recorder receives signal from mother board. The signal is processed and
then sent to printer.
The recorder consists of data buffering circuit, control circuit, motor driving
circuit, thermal printing.

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Data buffering: CPU processes data received and send it to print
Temperature test: Connector J3 receives temperature signal and output into
GAL module.

Problem and Solution

-- Problem: No paper out.
Solution: Check the motor drive chip LB833M. Replace it if it is not working.

2 Tubing System

Probe
Volume Control
Sampling Board
Assembly
Probe Washing Diluent Adding
Cover Assembly
Counting Cup
Valves

Filter

Figure 2-1

2.1 Tubing Functions

-- Dilution of specimen.
-- Cells counting and HGB measurement.
-- Declog, prime and clean.
-- Test of vacuum pressure.
-- Adding and discharge of diluent.
-- Adding of lyse.

2.2 Tubing Structure

2.2.1 Valves

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 Figure 2-2

Specification
-- Voltage: DC 24V
-- Power: 2.5W - 3.4W
-- Ambient Temperature: 0 - 45
-- Fluid Pressure: -90 - 200kpa

Functions
Valves functions are listed as Table 2-1.
Table 2-1 Valves function
No. Function
V1 Switch among RBC adding, mixing and discharging
V2 Switch among WBC adding, mixing and charging
V3 Switch between cup cleaning and measurement
V4 WBC measurement
V5 RBC measurement
V6 RBC mixing
V7 RBC measurement tube cleaning
V8 WBC measurement tube cleaning
V9 RBC measurement tube discharging
V10 WBC measurement tube discharging
V11 Liquid discharging after probe external wahsing
V12 Probe external surface washing with diluent
V13 Diluent adding
V14 Probe inner washing with diluent
V15 Diluent adding to WBC or RBC cup
V16 Lyse adding
V17 Negative pressure access

Problem and Solution

-- Problem: Sampling is not enough.

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 Solution: Valve12 or valve14 is blocked or air leakage. Check the valve and
replace it if malfunction.
-- Problem: Both sampling and discharging not performed.
Solution: The possible reasons are that the piston of dilutor wears down, or
the sampling needle is rusted, or the D motor lacks of a phase. Replace
related components can solve these problems.
-- Problem: The probe washing cover leaks.
Solution: Connection of valve11 and pipette is loose. Rectify the connection
to solve the problem.
-- Problem: WBC or RBC bubbles
Solution: Valve9 or Valve10 is not working normally. Check the connection to
find any air leakage.

2.2.2 Stepping Motor

Figure 2-3

Principle
Black

White ns

+V White

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 Red Blue Green

Q1 Q2 Q3 Q4

Figure 2-4 Electric diagram

Table 2-2 Engineering movement

Step Q1 Q2 Q3 Q4
1 ON OFF ON OFF
2 OFF ON ON OFF
3 OFF ON OFF ON
4 ON OFF OFF ON
1 ON OFF ON OFF

2.2.3 WBC and RBC Cup

Figure 2-5 WBC and RBC cup

2.2.3.1 Structure
The WBC cup is used for HGB measurement and WBC totals and diffs. RBC
is used for RBC and PLT totals.
The structure of cups assembly is shown as Figure 2-6.

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 Diluent Priming Tube
Diluent Priming Tube
Lyse Priming Tube

RBC Cup WBC Cup

Figure 2-6 Cups assembly

The structure of WBC cup is shown as Figure 2-7.

Chamber
HGB Receiver
Cover

HGB Tube HGB Send-out

Figure 2-7 WBC cup

The structure of RBC cup is shown as Figure 2-8.

RBC Cup

Mat Seal Ring

RBC Sensor Cover
Back Assembly

Figure 2-8

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 2.2.3.2 Installation

Replacement of Mat
Step:
-- Discharge all liquid and use a screwdriver to loose the two screws. It is a
better method to loose one a little bit and then the other to reach a parallel
screw out.
-- Carefully drag out the back assembly.
-- Use a nipper to get out the mats and the sensor as shown in Figure2-9.

Figure 2-9
-- Installation:
Use a nipper to pick up a mat and pack it into the hole, and then install the
sensor and the two mats. Pack the back assembly and tighten the screws.
-- Check out if any liquid leakage.
-- Use control to make modifications.

Replacement of Sample Cup

-- Take out cover, open left and front cover.
Front Cover

Cover

Left Cover

Screwdriver

Figure 2-10
-- Take out the cover with screwdriver.

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 Figure 2-11
-- Drag out HGB, RBC and WBC cables.
HGB Signal

WBC Signal

RBC Signal

Figure 2-12
-- Take out ground cable.

Ground Cable

Figure 2-13
-- Take out silica tube under sample cup with hand.

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 Silica Tube

Figure 2-14
-- Push sample fixed holder gently with hand, take out sample cup.

RBC Cup

WBC Cup

Figure 2-15
-- Take out hard tube of cup back chamber connector. Attention: hard tube
can not be folded.
NOTE: RBC cup is on the left side and WBC on the right side.
-- OUT of V1 connected to upside connector of WBC cup back chamber with
hand, NC of V2 connected to the downside connector of RBC cup back
chamber.
-- OUT of V13 connected to upside connector of WBC cup back chamber,
NC
connected to downside connector of WBC cup back chamber.

Upper Connector

Down Connector

Figure 2-16
-- Make RBC, WBC signal line through rectangle aperture subjacent sample

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cup fixing board.
-- Install RBC, WBC sample cup on cup fix holder.
-- Put silica tube to subjacent connector of sample cup.
-- Fix the ground line with screw and cross-screwdriver.
-- Smear silica gel at joint between sample cup connector subjacent and
silica tube.

2.2.3.3 Problem and Solution

-- Problem: PLT result is high.
Solution: RBC signal cable is not well connected. Reconnect the cable.
-- Problem: HGB result is 0.
Solution: HGB signal cable is not well connected. Reconnect the cable.
-- Problem: WBC or RBC alarm for ***
Solution: Lyse tube is blocked. Run declog.
-- Problem: Alarm for WBC clog.
Solution: WBC calculation channel is seriously blocked. Run declog.
-- Problem: PLT result is high.
Solution: RBC bubble in cup. Remove bubble.
-- Problem: WBC result is 0.
Solution: Wash WBC signal cable.
-- Problem: Background test cant be performed, and results are not stable.
Solution: Probably the cup is broken. Replace the cup can solve the problem.

2.2.4 Sampling Unit

Figure 2-17
Function
Main function is sampling, dilute and rinse during testing.

Structure
Sampling unit is composed of sample probe assembly (sample probe,
probe

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wiper, sample arm), transverse drive seat assembly (synchromesh belt,
motor D, switch, driven gear assembly); longitudinal drive seat assembly
(synchromesh belt, motor C, switch, driven gear assembly).
Sample probe assembly: Collect certain blood into sample cup for testing
through sample probe.
Transverse drive seat assemblyTrough transverse movement make sample
probe can reach sample cup easily.
Cross drive seat assembly: To ascertain up & down position trough
movement.

Figure 2-18
Problem and Reason
-- Problem: MC or MD error
Reason: KD failure
-- Problem: In pre-diluent mode, PLT data over-high
Reason: Interior of sample probe is dirty. Take out and dip in detergent.
-- Problem: Sample probe noisy during running.
Reason: MC failure
-- Problem: V15 spray
Reason: sample probe block
-- Problem: MC or MD error
Reason: Longitude belt of sample unit looses.
-- Problem: Repeatability of RBC test result is not so good.
Reason: There is blood on probe wiper.

2.2.5 Diluent and Lyse Adding Unit

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 Figure 2-19

Function
The assembly provides diluent and lyse for sampling, counting and cleaning.

2.2.5.1 Structure and Installation

The assembly is made up of stepping motor, piston, etc.

Stepping Motor
Check out the rotating shaft and connectors. Make sure that they can work
in a good status.

Installation of Pistons
-- Diluent piston: Pick up a seal ring and put it around the piston rod and then
insert the rod into cylinder. This way can automatically set the seal ring into its
position. Cover the cylinder with 4 screws.
-- Lyse piston: Insert one of the head of glass cylinder into A T-shape
connector and then fill the other space inside the connector with glue. Insert
the piston rod into the glass cylinder.

Install piston assembly with stepping motor

-- Fix 3 stepping motors onto the bracket.
-- Assemble the 3 pistons respectively by sequence of sample, diluent and
lyse.

2.2.5.2 Problem and Reason

-- Problem: Repetition is not good.
Reason: KB switch is not well connected.

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 -- Problem: Sampling needle doesnt run sampling and discharging.
Reason: Motor rod is broken or the T-shape connection is not good.
-- Problem: Very little even no diluent drain down.
Reason: The screw of motor is loose.
-- Problem: System sends wrong alarm for lacking of lyse.
Reason: Theres bubble inside lyse tubing.
-- Problem: Sampling volume is too little.
Reason: Lack of some motor phases.
-- Problem: Result is respectively lower.
Reason: leakage due to malfunction of diluent seal ring.

2.2.6 Vacuum Generation Unit

The vacuum pressure is generated by a pump which controls pressure
inside a chamber to generate pressure difference and thus controls the
liquid flows.
Structure and Installation
-- Vacuum pump: The vacuum pump is the dynamic source of liquid flow
inside the system. Its working voltage is DC 12 V.
The pump transports a gas load from an inlet port to an outlet port through a
rotating motor.

Figure 2-20
-- Vacuum chamber
The vacuum chamber is an enclosure from which gas is removed by a
vacuum ump. On its upper side there are two ports. One is linked to the
pressure sensor on control board to test vacuum pressure inside. When
abnormal vacuum pressure is read, the pump starts to work to add or
decrease pressure. The other port is linked to related liquid flows of counting
or cleaning.
-- Valve
Valve6 is used to switch vacuum and waste drain.

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 2.2.7 Problem and Solution
-- Problem: Overflow from sample cup.
Solution: Check connections of pump and pipette, or probably MOS pipe or
diode is broken. Otherwise, the pump is broken.

2.3 Work Chart

2.3.1 Startup

Needle reposition

System initialization
Adding detergent Adding detergent
Absorb detergent
into WBC cup into RBC cup

Priming lyse

Homing of sampling Cleaning tubing

Turn off the power
needle system
Cleaning WBC cup

2.3.2
Cleaning RBC cup
Shut

Do wn

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 Cleaning WBC cup Priming lyse Washing

Sampling needle back Clean tubing system Cleaning RBC cup

2.3.3 Priming

Detergent absorbed into sampling needle

Adding detergent into WBC cup

Adding detergent into RBC cup

Soaking

Liquid discharging

Adding lyse into cups

Sampling needle performs washing

Cleaning tubing system

Homing of sampling needle

2.3.4 Counting

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2.3.5 Lyse Adding

Lyse

Valve 16

Diluting & Adding unit

Sensor

WBC cup

Diluent

Valve 13

Diluent adding unit

Valve 12
piston)
Valve 14

Valve 15

WBC and RBC cups

2.3.6 Diluent Adding

2.3.7 Sampling and Discharging

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 Whole blood
mode Dilution and Sampling unit
(small piston)

Dilution and Sampling unit

(big piston)

Sample Sampling

Valve10 Valve10

Sample Cup
Valve8 Valve8

Valve11 Valve11

Predilute

2.3.8 Inner Cleaning of Sampling Needle

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 Diluent

Valve 13

Dilution and Sampling unit

Valve 12
piston)
Valve14

Injector

Sampling Needle

2.3.9 Outer Cleaning of Sampling Needle

Diluent

Valve 13

Dilution and Sampling unit (big piston)

Valve 12
piston)
Cleaning fit

Valve 11

Vacuum pump

Waste Container

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2.3.10 Sample Mixing

Valve 20 Valve 2 WBC cup

Pressure pump

Valve 6 Valve 1 RBC cup

2.3.11 Declog

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 Valve 4 WBC cup

Pressure pump Valve 3 Tubing

Valve 5 RBC cup

2.3.12 WBC Counting

Sample valve

WBC cup Valve 4 WBC glass tube Vacuum chamber

Bubble

Valve 10

2.3.13 RBC Counting

The liquid system used to measuring the RBC in the process of counting.

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 RBC chamber Valve 3 RBC glass tube Vacuum pump

Bubble

Valve 9

2.3.14 Measurement Unit Cleaning

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 Back chamber of
WBC cup Valve 3

Valve 8
WBC glass tube

Detergent Vacuum chamber

Valve 7 RBC Glass tube

Back chamber
of RBC cup Valve5

2.3.15 Vacuum Chamber Drainage

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 Vacuum Chamber

Valve 17

Pump

Waste container

3 Adjustment

3.1 Hardware Adjustment

3.1.1 Vacuum Pressure

Condition
If vacuum pump works and no clog or leakage exists inside tubing, negative
pressure adjustment has to be performed when:
-- Clog due to too long counting time
-- The system sends alarm of low vacuum pressure while the vacuum pump
is working normally.

Method
Adjust VR4 and VR5 on mother board.

3.1.2 HGB Voltage

Condition
The HGB voltage has to be adjusted when:
-- After maintenance or replacement of mother board.

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 -- HGB problems or bubbles happen.
-- Replacement of WBC cup.

Method
Adjust VR1 and VR3 on mother board referring to specifications.

3.1.3 Voltage Plus

Condition
The voltage plus has to be adjusted when:
-- After replacement of sample cup.
-- After replacement of cell sensor.
-- After replacement of mother board.

Method
-- Press Menu and select 7 System Set and then press and Ok. The
system will require password 3333 and press Ok. Select Graphic Debug.
After running test, the plus value can be readout as shown in Figure 3-1.

WBC Plus

RBC Plus

Figure 3-1
-- Use control to obtain a plus value and make comparisons.
-- If the plus value obtained from control is much different from real value,
the VR9 and VR7 potentiometer have to be adjusted by the following steps:
a. Open the left door and find a metal cover as shown in Figure 3-2.

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 Figure 3-2

Remove the cover and find the board below.

Figure 3-3

b. Turn right to add plus value and turn left to reduce.

3.2 Software Test

3.2.1 Self Test

Press Menu and select 7 System Set and then press and Ok. The
system will require password 3333 and press Ok. Test items are listed as
Table 3-1.
Table 3-1

Item Standard Function Part

HGB Blank 2.600.02V VR1
HGB Background 0.002V VR3
Negative Pressure 141kpa VR4

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 Valve Test On/Off Related Valve
Data Factor Calibration For Calibration
WBC Constant Current On/Off For Measurement
RBC Constant Current On/Off For Measurement

3.2.2 Motor Adjustment

Press Menu and select 7 System Set and then press and Ok. The system
will require password 3333 and press Ok. Now press Shift and Menu all
motor adjustment data will be readout as listed in Table 3-2.
Table 3-2 Motor adjustment
Item Description Set Value
X2wbc Step of the probe to WBC cup 1215
X2rbc Step of the probe to RBC cup 865
Ymbld The depth of the probe out of the machine 750
Y2wbc1 The fall steps of probe to WBC cup 1 550
Y2wbc2 The fall steps of probe to WBC cup 2 550
Smbld Steps of sample syringe taking the blood 400
Smwbc Absorb steps of sample syringe to WBC cup 585
Dmin The volume of diluent syringe dispense to the 1462
Dmout Volume of diluent syringe pore outside the 480
Dmpin Volume of diluent syringe take from 230

D2wbc Volume of diluent syringe pore to counting cup 2250

D2rbc Volume of diluent syringe pore to counting cup 2250
D2wbc-d Volume of diluent syringe pore to counting cup 2250
D2rbc-d Volume of diluent syringe pore to counting cup 2250

Lhuman Volume of Lyse syringe dispensing 3500

Lhuman-d Volume of Lyse syringe dispensing(diluted mode) 3500
Ldog Volume of Lyse syringe dispensing 3500
Lhorse Volume of Lyse syringe dispensing 3500
Lcat Volume of Lyse syringe dispensing 3500
Lchicken Volume of Lyse syringe dispensing 3500
Lother1 Volume of Lyse syringe dispensing1 3500
Lother2 Volume of Lyse syringe dispensing2 3500

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 Lother3 Volume of Lyse syringe dispensing3 3500
Pressure Negative pressure 15
Airtime Mixture time 0
w-L-time Lower limit of WBC alarm 60
w-h-time Upper limit of WBC alarm 160
r-L-time Lower limit of RBC alarm 80
r-H-time Upper limit of RBC alarm 240
w-limit WBC terminus 20
r-limit RBC terminus 10
p-limit PLT terminus 12

Note:
-- The above value is only for reference, subject to change of different wares.
-- Rotation speed: 180steps/sec.

4 Final Test
After maintenance the engineer must run the device to check its function.

4.1 Startup
-- Turn on power supply.
-- Observe mechanical movement of all parts and make sure that steps
are
normal and smooth.
-- Check all lights. Green indicates the device is ready while orange indicates
the system is counting cells.
-- Check screen brightness.
-- Set up system date and time.
-- Check the function of transmission between Whole blood mode and
predilute mode. System will send out Di if the mode transmission is
successful. Blue light indicates whole blood mode and off light indicates
predilute mode.

4.2 Declog and Prime

Press Menu and choose Declog. Check the liquid drainage from sample cup
for any leakage or clog or abnormal sound.

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 4.3 Background Test
-- Press Menu and run priming for 2 to 3 times.
-- Repeat test for 10 times and record results. The final results have to meet
specifications.

4.4 Calibration
-- Run control under whole blood mode and predilute mode.
-- Compare real results and control results. The difference has to meet
specifications.
-- 15 tests are necessary for bother whole blood mode and predilute mode.
Accuracy and repeatability have to meet specifications.

4.5 Printer Test

-- Take out paper from printer.
-- Press Print and check if the system sends alarm for lacking of paper.
-- Install paper into the printer again and now the alarm should be dismissed.
-- Print a report and check its format.

5 Spare Parts List

Table 5-1 Spare parts list
No Code Description
Co HB-7021 Control board
1 HB-7021-YH-001
2 HB-7021-YH-002 MTB board

3 HB-7021-YH-003 Recorder control board

4 HB-7021-YH-004 Film pad

5 HB-7021-YH-005 Switch power

6 HB-7021-YH-006 Transformer

7 HB-7021-YH-007 LCD

8 HB-7021-YH-008 Key-press sticker

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 9 HB-7021-YH-009 Recorder Assay

10 HB-7021-YH-010 WBC Chamber Assay

11 HB-7021-YH-011 RBC Chamber Assay

12 HB-7021-YH-012 Dilutor dispensing Unit

13 HB-7021-YH-013 Vacuum chamber unit

14 HB-7021-YH-014 Liquid-store pot

15 HB-7021-YH-015 Step motor A

16 HB-7021-YH-016 Step motor B

17 HB-7021-YH-017 Motor of sampling unit

18 HB-7021-YH-018 Switch

19 HB-7021-YH-019 Double voltage Valve

20 HB-7021-YH-020 Liquid pump

21 HB-7021-YH-021 Gyral pumpAir pump

22 HB-7021-YH-022 3-way valve

23 HB-7021-YH-023 2-way valve

24 HB-7021-YH-024 Sampling probe

25 HB-7021-YH-025 Cleaning set

26 HB-7021-YH-026 Small O-ring of dilutor

27 HB-7021-YH-027 O-ring

28 HB-7021-YH-028 Small piston

6 Appendix

6.1 Counting Time

The counting time refers to the time that sample runs from the upper optical
coupler to the bottom coupler. The time is affected by the following factors:
-- The inner diameter and thickness of cells sensor inside sample cup.
-- The inner diameter and length of glass tube.

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 -- The length between upper and bottom optical couplers.
-- Leakage of tubing.
Normally WBC counting time is 10s-14s and RBC counting time 17s-22s.
If counting time is longer than set time, system will send alarm for clog while if
counting time is shorter, system will send alarm for bubbles.

6.2 Specification on Maintenance

Ground connection resistance 0.1
Regular leakage current to ground 0.5MA
Single malfunction leakage current to ground MA
Power source and outer cover can resist electricity of 1500V 50Hz

6.3 Electric Diagram

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END

CLINDIAG SYSTEMS B.V.B.A.

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