Antimicorbial Substance Microbiology-Lab Subject of Study 7

Surface Agents
Disinfectants –Chemical substance used to kill/inhibit the growth of infectious
organisms in inanimate objects
Antiseptic – Chemical substance used to kill/inhibit the growth of infectious
organisms of living tissue
Chemotherapeutic Agents: chemicals used to teat disease.
Antibiotics: substance naturally produced by a bacteria and fungi that kill/inhibit the
growth of other microorganisms.
Synthetic Drugs: chemical substance synthesized in the laboratory (sulfa compound)

Antimicrobial Agent
1 2 3 4 5 6
Bacterial Species 70% 5%
Hydrogen Isopropyl Chlorine Mouth
Iodine Peroxide Alcohol Bleach Wash Salt
P. aeruginosa No Zone No Zone
(Gram -)

S. aureeus No Zone No Zone

(Gram +)

Equipment:
Sensi-disc dispenser
Bunsen Burner
Millimeter ruler
Petri dish
Kirby-Bauer Antimicrobial Sensitivity Test

Gram – (Antibiotic) P. aeruginosa

zone Susceptibility
size
Penicillin 0 Resistant
Steptomycin 8 Resistant
Tetracycline 15 Intermediate
Gentamicin 14 Intermediate
Vancomycin 0 Resistant
Erythromycin 20 Intermediate
Antimicorbial Substance Microbiology-Lab Subject of Study 7

Gram + (Antibiotic) S. aureus

zone Susceptibility
size
Penicillin 14 Resistant
Steptomycin 13 Intermediate
Tetracycline 25 Susceptible
Gentamicin 21 Susceptible
Vancomycin 16 Susceptible
Erythromycin 25 Susceptible
All Gram + = Susceptible

Zone of Inhibition

Antibiotic Disk

Millimeter

Bacterial Lawn R