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Description

Designation:MacLeod
DepositedName:Escherichiacoli(Migula)CastellaniandChalmers
ProductSheet
Propagation
Escherichiacoli(ATCC
10536) Medium
ATCCMedium3:Nutrientagarornutrientbroth

GrowthConditions
PleasereadthisFIRST Temperature:37C
Atmosphere:Aerobic
StorageTemp.
PropagationProcedure
Frozen:80Cor 1. Openvialaccordingtoenclosedinstructions.
colder 2. Usingasingletubeof#3broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror


FreezeDried:2C 1.0mLpipette.Rehydratetheentirepellet.
to8C 3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.
LiveCulture:See 4. Useseveraldropsofthesuspensiontoinoculatea#3agarslantand/orplate.
5. Incubatethetubesandplateat37Cfor24hours.
Propagation

Section
Notes

BiosafetyLevel Depositorstatesthatthisstrainisusedintheassayofcolistin[colimycin],rifamycins,tetracycline,

1 spectinomycin[actinospectacin],andchloramphenicol.ATCCdoesnotperformsusceptibilitytestingfor
theseantibiotics.
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

IntendedUse
References

Thisproductisintendedforresearchuseonly.Itisnot
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
BiosafetyLevel:1
CitationofStrain
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
Ifuseofthiscultureresultsinascientificpublication,it thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
shouldbecitedinthatmanuscriptinthefollowing DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
manner:Escherichiacoli(ATCC10536) forHealth.

ATCCWarranty

ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein
mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
longervalid.

Disclaimers

Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
AmericanTypeCultureCollection
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
POBox1549 patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
Manassas,VA20108USA confirmedtobeaccurate.
www.atcc.org
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
800.638.6597or703.365.2700
Fax:703.365.2750 ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
Email:Tech@atcc.org themisidentificationormisrepresentationofsuchmaterials.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
Orcontactyourlocaldistributor
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

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AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2014.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[11/21]

ProductSheet
Escherichiacoli(ATCC
10536)

PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder

FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section


BiosafetyLevel
1

IntendedUse

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain

Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Escherichiacoli(ATCC10536)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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