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NEW PROBLEM
A 10,000 liter (of liquid) bioreactor contains 5g/L of growing cells, QO2 = 20 mmol O2 / (g
cells.hr),DT = 2 m, Di = 1 m, (6 - blade turbine agitator) x 3 blades. For 1 liquid volume per minute
aeration rate (air), can the OTR(oxygen transfer rate) = OUR(oxygen uptake rate) for N = 100 rpm?
GIVEN:
VL=10,000L
QO2 = 20 mmol O2 / (g cells.hr)
DT = 2 m
Di = 1 m
(6 - blade turbine agitator) x 3 blades
N = 100 rpm
REQD: Is OTR = OUR?
SOLN:
Re = Reynold's Number= LNDi2 / L
L= 1000 kg/m3
L =10-3 N.s/m2
Re = 1.67x10-6
NA = (10,000Lpm)(10-3m3/L) / (100min-1)(1m)3
NA = 0.10
Pm = (0.42)(5.62x104 Watts)
Pm = 2.335x104 Watts = 31.3hP
=0.0263 atm
*Since OUR > OTR, we must modify the bioreactor operation in order to bring them into balance
increase N
http://www.chem.mtu.edu/~drshonna/cm4710f07/lectures/chapter10.pdf
CHAPTER 2: ENZYME KINETICS
NEW PROBLEM
The following data were obtained for the reaction A B, catalyzed by the enzyme Aase. The
reaction volume was 1mL and the stock concentration of A was 5.0mM. Seven separate reactions were
examined, each containing a different amount of A. The reactions were initiated by adding 2.0L of a
10M solution of Aase. After 5 minutes, the amount of B was measured.
(a) Calculate the initial velocity of each reaction (in units of M.min-1)
(b) Determine the KM and Vmax of Aase from a Lineweaver-Burk plot.
(c) Calculate kcat.
SOLN:
Reaction o(M.min-1)
1 5.2
2 5.8
3 7.8
4 8.6
5 11.2
6 12.4
7 14.2
Vmax = 16 M/min
KM = 83 M
https://ww2.chemistry.gatech.edu/~lw26/bCourse_Information/3511/stud_comp/chap12_17.pdf