721N Spectrophotometer

Operating Manual
 TABLE OF CONTENTS

1 APPLICATIONS AND FEATURES .................................................... 3

2 SPECIFICATIONS AND STANDARD ACCESSORIES ......................... 4

3 PRODUCT APPEARANCE AND FUNCTION KEYS ............................. 6

4 INSTALLATION ............................................................................. 9

4.1 INSTALLATION ENVIRONMENT ........................................................... 9

4.2 CHECK-UP WHEN UNPACKING ............................................................ 9
4.3 INSTALLATION ............................................................................ 10

5 OPERATIONS .............................................................................. 11

5.1 BASIC OPERATIONS ...................................................................... 11

5.1.1. Warming up ........................................................................... 11
5.1.2. Zero adjustment ..................................................................... 11
5.1.3. Adjust 100%T ........................................................................ 11
5.1.4. Wavelength measurement........................................................ 12
5.1.5. Change the groove position to get sample into optical path. ......... 12
5.1.6. Change the scales ................................................................... 12
5.2 PRACTICAL OPERATIONS ................................................................ 13
5.2.1. Measure the transmittance of transparent materials .................... 13
5.2.2. Determine the transmittance curves of transparent materials ....... 13
5.2.3 Determine the transmittance of transparent solutions ................... 14
5.2.4. Do quantitative measurement with standard curve method .......... 14
5.2.5. Use the direct reading ............................................................. 15

6 MAINTENANCE AND ERROR RECOGNITION ................................ 17

6.1 DAILY MAINTENANCE .................................................................... 17

6.1.1 Notice ................................................................................. 17
6.1.2 Inspect the instrument .......................................................... 17
6.1.3 Replace the source lamp........................................................ 17
6.1.4 Clean the liquid storage container ........................................... 18

1
6.2 CHECK THE FUNCTION OF MAIN DEVICE ............................................. 18
6.2.1 Check the wavelength range .................................................. 18
6.2.2 Check the transmittance repeatability ..................................... 18
6.2.3 Check fixed-point noise ......................................................... 18
6.2.4 Check the wavelength repeatability ......................................... 19
6.3 IDENTIFYING AND HANDLING THE COMMON MALFUNCTIONS ................... 19

2
1 Applications and Features

721N visible spectrophotometer, compact and easy-to-use spectrophotometric equipment,

can be used to measure transmission, absorption and concentration direct-reading at

wavelengths from 360nm to 1000nm. It can be widely applied to departments related to

medical health, clinical examination, biochemistry, petro-chemistry, environmental

monitoring and quality control as qualitative and quantitative analyses. The main features

are the following:

4-digit LED display

Reasonable structure with advanced optical system, using precision machining

high-quality CT monochromator and sealed grating, the optical properties of which

are superior to the traditional LITTROW monochromator, with obvious advantages in

the indicators such as resolution, photometric accuracy, stray light and stability. And

its size is smaller.

Special precision pre-adjustment lamps and lamp holder fittings. Not necessary to

re-adjust optical path for installation which is convenient for users without any

professional maintenance skills.

Precision-linkage cutoff filter system automatically switching to appropriate

wavelength filters, further reducing stray light

Sample cells made of special engineering plastics, resistant to solvent as well as

strong acid/alkali, which enhances its anti-corrosion properties. Effusion dish and

liquid storage container, which can be removed and cleaned, are mounted on the

bottom to increase the service life.

4-position colorimetric cell shelf, with optional rectangular optical path colorimetric

dish with diameter from 1 cm to 5cm.

Automatic zero and 100%T adjustment

Concentration factor setting and concentration direct-reading functions

3
2 Specifications and Standard Accessories

1. Optical system: diffraction grating C-T monochromator

2. Wavelength range:360nm1000nm

3. Source lamp: halogen lamp 10W/8V

4. Wavelength accuracy: 3.0nm

5. Wavelength repeatability: 1.5nm

6. Transmittance accuracy: 0.8 %() (SRM930D)

7. Transmittance repeatability: 0.3 %()

8. Spectrum bandwidth: 6nm

9. Stray light: 1.0 %() (360nm, NaNO2)

10. Display scale: (T):0.0199.9%

(A):-0.32.999

(F):19999

(C):09999

11. Power supply: 90250V, 50/60Hz, 40W

12. Dimension: 350mm300mm220mm

13. Weight: net weight: 4kg; gross weight: 6kg

14. Basic set:

1. Main device: 1pc

2. Power cord: 1pc

3. Operating manual: 1pc

4. Product Quality Certificate: 1pc

5. Fuse (2A): 2pcs

6. 1cm rectangular colorimetric dish (glass): 4pcs

7. 1cm optical path colorimetric dish shelf: 1pc

8. Packing list: 1pc

15. Optional spare parts

1. Fuse (2A/3A)
2. Illuminant light components with pre-calibration lamp bracket

4
3. Rectangular colorimetric dish: 1cm, 2cm, 3cm, 5cm
4. 5cm optical path colorimetric dish shelf
5. Pr-Nd filters
6. Holmium trioxide filters

5
3 Product Appearance and Function Keys

1. /100% key: automatically adjusts 100%T when

"transmittance lamp is bright

(Press once again if the first press doesn't work);

automatically adjusts absorbance 0 when

"absorbance lamp lights

(press once again if the first press doesn't work);

increases concentration factor settings when

"concentration factor lamp is bright. Press for 1

second, then the values increase rapidly, then

press MODE to confirm set values automatically;

increases concentration direct-reading settings

when "direct-reading lamp lights. Press for one

second, the values increase rapidly, and then

press MODE to confirm set values automatically.

2. /0% key: automatically adjusts 100%T (range<10%T)

when "transmittance lamp lights;

*Don't use "absorbance" lamp when it is bright,

otherwise itll be overloaded;

reduces concentration factor settings in the

same way as for /100% when "concentration

factor lamp lights

reduces direct-reading settings in the same way

as for /100% when "direct-reading method

lamp lights.

3. FUNCTION key: is used as an extension functional key for

presetting

4. MODE key: selects display scales

6
 lamps lights up in the sequence of

"transmittance, "absorbance, "concentration

factor, and "direct-reading, press to start

another circle of lightening lamps.

5. Display window 4-digit LED number Displays data and error information

6. "Transmittance lamp: displays transmittance data

7. "Absorbance lamp displays absorbance data

8. "Concentration factor lamp: displays concentration factor data

9. "Concentration direct-reading lamp: displays direct-reading data

10. Sample cell: tests samples

11. The pull rod of the groove shelf: changes the position of sample groove (4)

12. Wavelength display window: displays the wavelength

13. Wavelength regulation button: adjusts the wavelength

14. Power socket: connects the power cord

15. Main switch: ON/OFF power supply

7
 6 5
7 4
8 3
9 2
2
1

12 13
10 11

14 15

8
4 Installation

4.1 Installation environment

This instrument is suitable for analytical experiments under lab conditions, the following
requirements are demanded due to the fact that it works with PC:

4.1.1 Temperature: 5 35 , relative humidity is less than 85%.

4.1.2 It is mounted on a steady working platform, free from vibration, direct sunlight,

strong electromagnetic field interference, dust and corrosive gas.

4.1.3 Power voltage:90250V Frequency:50/60Hz

4.1.4 Clean the instrument surface with room temperature water, do not use cleaning

solvents such as alcohol or acetone

4.1.5 If the equipment is to be used on the spot, please use the original package while

being moved. On-site working environment usually complies with the above

requirements. Please make special orders if users have special requirements.

4.2 Check-up when unpacking

4.2.1 Note: the instrument and computer are packed in paper cardboard boxes

(wooden boxes are required for long-distance transportation.

4.2.2 Please check the package before unpacking. If the package is incomplete or

Collision traces are found, please contact the insurance and transportation

department.

4.2.3 Open along the sealing tapes; carefully remove the main device and computer

(please save the outer package for the later using). Check the main device's

Standard and optional components according to the packing list. Contact the

area sales or our company if there is any problem.

9
4.3 Installation

4.3.1 Remove the fixing tapes which are for transportation, clean the surface, then

check the power switch at the bottom of the device. Make sure voltage

requirement is consist with the local power supply, then put the main device on

a stable working station, keeping clearance more than 10 cm away from the

wall. Connect power cord to the outlet at the lab.

10
5 Operations

5.1 Basic operations

5.1.1. Warming up

After the instrument is started, lamps and electric components need heat balance;

therefore, measurement will be done in 30 minutes after it is warming up. For

urgent usage, adjust 0%T or 100% any time.

5.1.2. Zero adjustment

Purpose: adjusts both sides of the basic scale (along with 100%T adjustment),

enter the correct testing module;

Timing adjustment: after warming up, change measuring wavelength or having

tested for a period; or before high precision measurement;

Operation: open the sample cover (automatically shut the light valve) or shade

the optical path with opaque materials in the sample cell, then press 0%, then

zero is automatically adjusted.

5.1.3. Adjust 100%T

Purpose: adjusts both sides of the basic scale (along with zero adjustment), and

enters the correct measuring status;

Timing adjustment: after warming up, change measuring wavelength or testing

for a period or before high precision measuring (generally, do 100%T adjustment

before zero adjustment as to make the automatic increase inside the device to the

full reach);

Operating: empty sample used as background is put into the light valve of the

sample cell, shut the sample cover (automatically open the light valve), press

100% to automatically adjust 100%T (press once more if error occurs);

Note: when adjusting 100 T, the automatic gain system re-adjustment may

11
 influence 0%T. Check 0%T after adjustment. If there's any change, re-adjust 0%

for once.

5.1.4. Wavelength measurement

Use the only knob on the equipment to adjust the current measuring wavelength.

The wavelength displays on the window to the left of the knob. Read the readings

vertically.

Note: this machine uses precision-linkage cutoff filters system, so when the knob

rotates, there will be metal gratings. Low gratings at 360nm-1000nm are normal.

5.1.5. Change the groove position to get various samples into optical path.

In the standard specification, the testing sample groove shelf has four positions.

It can be controlled by the pull rod on the front of the device. Open the sample cell

cover to observe the positions: the closest one to the user is 0, then 1, 2, 3

in sequence. The corresponding pull rod reaches 0 by pushing backward to the

end, then pull 1, 2, 3 outward respectively. When the rod is at the right

position, the user may feel it. Please push it a bit to make sure the position is right.

5.1.6. Change the scales

This equipment has four scales:

Transmittance: measures the transmittance features of transparent liquids and

solids;

Absorbance: uses as quantitative analysis with standard curve method or

absolute absorption spectrometry; also used in kinetic tests;

Concentration factors: sets concentration factors when direct reading with the

concentration factor method;

Concentration direct-reading: sets and reads with standard sample method to

read directly; also directly reads the concentration when setting concentration

factors;

12
 Use MODE to adjust the scales, which displays with the lamps for transmittance,

absorbance, concentration factor, direct-reading respectively, the initial

status is transmittance, press to select by following the above procedures.

5.2 Practical operations

5.2.1. Measure the transmittance of transparent materials

Warm up 5.1.1

1 Set wavelength 5.1.4

2 Set the blank 5.1.5

3 Set the scale to "transmittance 5.1.6

4 Adjust 100%T 5.1.3

5 Adjust zero 5.1.2

6 Adjust 100%T 5.1.3

7 Add samples 5.1.5

8 Read data

5.2.2. Determine the transmittance curves of transparent materials

Within the required wavelength to be measured, repeatedly execute this method according
to the required intervals in phase 1-8 of section 5.2.1, and write down the responding
transmittance values on the paper, to display the transmittance curves.

13
5.2.3 Determine the transmittance of transparent solutions

Warm up 5.1.1

1 Set wavelength 5.1.4

2 Set the blank 5.1.5

3 Adjust 100%T0%T 5.2.1 5-7

4 Set the scale to absorbance 5.1.6

5 Add the sample in the optical path 5.1.5

6 Read data

5.2.4. Do quantitative measurement with standard curve method

1 Get standard samples with known content

2 Prepare sample solutions and background solutions

due to rules of analysis

3 Set wavelengths, blanks, zero adjustment, 5.2.3 1-6

"absorbance", and read sample absorbance

4 Repeat the above procedures and read the 5.2.3 2-6

samples' absorbance

5 Read the absorbance, draw the coordinate maps

based on samples' known contents, and draw the
best relative curves

6 Read the unknown samples' absorbance, and find 5.2.4 -3

14
 responding concentration on the curve

5.2.5. Use the direct reading

When the analysis procedure is stable and the basic standard curve passes the original
point, users apply direct-reading to do quantitative measurement instead of through
complex standard procedures. This method needs to prepare one sample solution with
concentration at 2/3 of the quantitative range. The operation is as follows:

1 Measure the absorbance of standard samples 5.2.4 phase 1-3

2 Set the scale at concentration direct-reading 5.1.6

3 Press or to set the reading at the known

content(or 10 times of contents)

4 Add unknown sample solutions 5.1.5

5 Read the displaying data, i.e. The content value(or 10

times of contents

5.2.6. Use concentration factors directly

After the 3rd procedure, if the scale is set at concentration factor, the reading in the
display window is the sample's concentration factor. Record the factor, then users read the
figure without measuring it again until is switched off. The procedure is as follows:

1 Switch on, warm up, set the wavelength, set the

background solution, adjust 0% and 100%T

2 Set the scale at concentration factor 5.1.6

3 Press or to make the display value is the

factor value

4 Set the scale at concentration direct-reading 5.1.6

5 Set unknown sample solution 5.1.5

15
6 Read the displaying value, i.e. the concentration
value

16
6 Maintenance and error recognition

6.1 Daily maintenance

6.1.1 Notice

6.1.1.1 Comply with requirements in 4.1 for daily use;

6.1.1.2 Do not use organic solvents such as ethanol-ether to clean the

equipment. Use a cover when it's not used;

6.1.1.3 Clean the colorimetric dish with petroleum ether after using, and dry it

with lens paper, is kept colorimetric dish box for the next use.

6.1.2 Inspect the instrument

It is necessary to open the cover when it is required to check up the device, including the

inner structures, optical path, and electric circuit, or to replace the source lamp. The

procedures are as follows:

6.1.2.1 Power off;

6.1.2.2 Open the wavelength knob cover and remove the knob;

6.1.2.3 Remove five M4 screws from the bottom of the equipment, open the

cover to check the equipment (avoid touch the high-voltage part on the

back of the device base).

6.1.3 Replace the source lamp

The equipment uses the long-service combining source lamp pre-corrected by the factory.

Replace the damaged lamps based on the following steps:

6.1.3.1 Open the upper cover (see 6.1.2);

6.1.3.2 Remove the old illuminant light's parts: remove the connecting cable, 2

M4 screws on the lamp shelf, and other parts;

6.1.3.3 6.1.3.3 Re-install the new illuminant parts according to the reverse order

as that in 6.1.3.2; observe the reflector at front, adjust the position of

17
 the parts, and make sure the rectangle spots in the middle of the incident

slit.

6.1.4 Clean the liquid storage container

6.1.4.1 Lift the equipment up a little, and move the front end out of the desk;

6.1.4.2 Take out the container on the bottom (beware of liquids flowing out

during this procedure);

6.1.4.3 Dump the liquid, clean the container with water and dry it;

6.1.4.4 Mount the container back to the original place;

6.1.4.5 Put the equipment back to the original place.

6.2 Check the function of main device

After the equipment finishes testing and maintenance, use the following to check to make

sure the equipment has the standards.

6.2.1 Check the wavelength range

6.2.1.1 Switch on the host and pre-heat for 30 min, the mode is on

transmittance;

6.2.1.2 Turn the wavelength knob to both sides of the wavelength range, press

100%, 100%T will be achieved. Open the sample cell door, 0% can adjust to 0%T.

6.2.2 Check the transmittance repeatability

6.2.2.1 Set the host's wavelength to 550nm, equipment to 0%T and 100%T.

6.2.2.2 Set the transmittance to 40%T and check the display value around the

samples which are evenly absorbed nearby successively for three times (i.e. neutral

filters). The maximal difference should be between0.3%T.

6.2.3 Check fixed-point noise

6.2.3.1 Set the wavelength to 550nm, and adjust to 0%T and 100%T.

18
6.2.3.2 Set the scale to absorbance.

6.2.3.3 Observe the data in the window, which should be within the range of

0.002A.

6.2.4 Check the wavelength repeatability

6.2.4.1 Set the scale to transmittance.

6.2.4.2 Use the (Pr-Nd) filters (optional accessories, provided by ordinary

companies) as samples.

6.2.4.3 Regard air as blank, adjust the equipment to 0%T and 100%T, add the

sample into the optical path, read the responding wavelength values to standard peak

value among 520nm540nm.

6.2.4.4 Repeat 6.2.4.3 three times, the wavelength reading errors should be

1.5nm.

6.3 Identifying and handling the common malfunctions

Malfunctions Causes solutions

1) The equipment 1. Not connected to the 1. A Check the plug, should be
is powered on, but power well connected between
is not on 2. Fuses broken 90~250V
3. Connectors loose B Check if the power cable
breaks
C Check if the power plug breaks
2. Replace fuses
3. Re-plug plug-ins
2) Instable data 1. Not enough pre-heating 1. Warm up for 30 mins
display time 2. Maintain the power at 90
2. Unstable AC power 250V without sudden change
3. Drastic vibration around 3. Change working environment
the working environment 4. Open the cover and re-plug all
4. Bad plug-in connections plug-ins

19
3) Energy not 1. Illuminant light not light 1. Broken illuminant light to be
measured 2. The light valve not open changed, or no voltage output
3. The colorimetric ware 2. Check if the light valve works
completely cover the light 3. Put in at the right position
4. No signal output 4. Receiver broken to be
changed, or not plugged in, or
back contact
4) Cannot adjust 1. Not enough light energy 1. Select the right gain key;
to 100%T 2. Colorimetric dish not in check the light from the
position illuminant light in the incident
slit; low light voltageadjust
higher
2. Adjust it to the right position
5) Abnormal 1. Wrong solutions to the 1. Dealt with properly
photometry samples 2. Reduce matching errors
2. Colorimetric dish not 3. Check with (Pr-Nd) glass and
match adjust wavelength
3. Big wavelength errors
6) Err3 displays 1. Wrong operation 1. Power off then on again

20