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1 Prepare TOF
1.1 Load autotune method ESIautotune.m
1.2 Check the calibration coefficients and prints report
1.6 Click Autotune to initiate a Initiates automatic tuning, which automatically changes
the TOF parameters and calibrates the TOF
Note: If Autotune is initiated after vent and pump down again, Initial box must
be checked
1.5 manual tune is applied for specific applications
Gas temperature
Drying gas flow rate
Nebulizer
Capillary voltage
Fragmentor
Skimmer
2.3 Set up reference mass correction
Note: Enter an injection volume on this screen only if you want to override
the value in the method. Otherwise, use the default value of -1
3.6 Click the Start icon to run sample
3.7 Review the single run data