Description

Source:Desulfovibriodesulfuricanssubsp.desulfuricans(Beijerinck)KluyverandvanNiel
Designation:GenomicDNAfromDesulfovibriodesulfuricanssubsp.desulfuricansstrainMB[ATCC
NucleicAcidProductSheet 27774]
Description:GenomicDNAisolatedfromDesulfovibriodesulfuricanssubsp.desulfuricansStrainMB.This
Desulfovibriodesulfuricans bacterialstrainisalsoavailableasATCCCatalogNo.27774.
Note:GenomicDNAisolatedfrombacteriaisappropriateforPCR*andothermolecularbiologyapplications.
subsp.desulfuricans
*Thepolymerasechainreaction(PCR)processiscoveredbypatentsownedbyHoffmannLaRocheInc.Use
(Beijerinck)Kluyverandvan ofthePCRprocessrequiresalicense.

Niel(ATCC27774D5) BatchSpecificInformation

RefertotheCertificateofAnalysisforbatchspecifictestresults.
PleasereadthisFIRST
PreparationProcedure

BiosafetyLevel Centrifugetubepriortoopeningtopreventlossofpelletedmaterial
1
1. RehydratecontentsofvialwithmoleculargradeH2O.
2. Placevialat37Cfor1hourorat+2Cto8Covernight.
3. FormorecompleterehydrationandtofullyrecoverDNAincubatethesampleovernightat4Cwhile
IntendedUse rockingthenincubatefor1hourat65C.ResuspendingthedriedDNAin250Lmaygivebetter
results.
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor QualityControlInformation
diagnosticuse.
1. BacterialgenomicDNAisprovidedinadriedform.Storeat+2Cto8Cuponreceipt.Storeat20Cif
CitationofStrain storedformorethan6months).
Note:Donotstoreinfreezerswithadefrostcycle.Thiswillexposetheproducttoincreased
Ifuseofthiscultureresultsinascientificpublication,it temperatures.
shouldbecitedinthatmanuscriptinthefollowing 2. ConcentrationbyPicoGreenmeasurementwasfoundtobeapproximately5g.
manner:Desulfovibriodesulfuricanssubsp. 3. Purity:OD260/OD280ratio.
desulfuricans(Beijerinck)KluyverandvanNiel
4. IntegrityofDNAwasdeterminedbyelectrophoresisona1%agarosegelstainedwithSYBRSafe,
(ATCC27774D5)
andwasfoundtobeofhighmolecularweight.
5. NoRNAwasdetectedbyelectrophoresis.
NucleicAcidInformation
6. FunctionalactivitywasconfirmedbyPCRamplification16SribosomalRNAgene.
7. Identityconfirmedbysequencingof16SribosomalRNAgene(first~500basepairs).

TotalDNA:Atleast5gin1XTEbuffer.
BiosafetyLevel:1
OD260/OD280:1.6to2.1

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.

AmericanTypeCultureCollection Disclaimers
POBox1549
Manassas,VA20108USA
www.atcc.org Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproduct
800.638.6597or703.365.2700 sheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliterature
Fax:703.365.2750 andpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhas
Email:Tech@atcc.org

beenconfirmedtobeaccurate.
Orcontactyourlocaldistributor Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.
ATCCisnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.While
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 reasonableeffortismadetoinsureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefor
damagesarisingfromthemisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

NucleicAcidProductSheet AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2015.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[06/05]
Desulfovibriodesulfuricans
subsp.desulfuricans
(Beijerinck)Kluyverandvan
Niel(ATCC27774D5)

PleasereadthisFIRST

BiosafetyLevel
1

IntendedUse

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain

Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Desulfovibriodesulfuricanssubsp.
desulfuricans(Beijerinck)KluyverandvanNiel
(ATCC27774D5)

NucleicAcidInformation

TotalDNA:Atleast5gin1XTEbuffer.
OD260/OD280:1.6to2.1

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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