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DATED MATERIAL
Family of products you can rely on
Please Open
VERSION #3 continued from page 7
complimentary issue of LabNotes. kinase and leucine aminopeptidase mixing and proper anticoagulation
1985;109;247-249.
4. Brunson D. Smith, Bak A, Przyk E, Sheridan B, Muncer DL,
Comparing Hematology Anticoagulants; K2EDTA vs
K3EDTA. Lab Hematology 1995; 1:112-119.
Systems, The Hemolyzed Specimen:
determinations. of the specimen. Preanalytical
Causes, Effects, and Reduction
5. VS5244 (white paper), BD Vacutainer Tube Comparison:
To continue receiving free copies of this newsletter, It is important that the proper
Plastic K2EDTA vs. Glass K3EDTA Tubes for Blood Counts
on the Coulter MAXM. Solutions
please send us this postage-paid reply card and we will amount of blood be drawn into EDTA
6. VS5324 (white paper), BD Vacutainer Plus Tube
13 x100mm with Spray-Coated K2EDTA; An Evaluation of
gladly add you to our subscriber list. Visual Hemolysis, Foaming, Clotting and Selected
tubes, with the caution to avoid short Hematology Parameters.
draws. An excess of EDTA in the Our internal studies showed no By Valerie Bush, PhD. The issue of hemolysis has always The high degree of variability in
Lynn Mangan, MT, (ASCP)
01/03 VS7044
blood may increase osmotic pressure plagued clinical laboratories and the training, skills, and frequency
clinically significant differences See
and distort or rupture cells. Excess when comparing K3EDTA glass For In Inside continues to be a growing concern. of phlebotomy practice of the non-
On Ho structions In many hospitals, nurses and other
Your feedback is appreciated!
EDTA causes shrinkage of red cells tubes to K2EDTA plastic tubes. w To laboratory staff is a major factor in
healthcare workers have replaced
Your F Obtain the increase of hemolysis rates in many
We welcome your comments. Please describe specific subject matter that you
YES, I would like a complimentary subscription to
crit, mean corpuscular volume (MCV) cripti phlebotomists. Often this decentral- facilities. Hemolysis, defined as red
# of Beds
on
ization, as it is called, occurs with blood cell breakdown and the release
MI
Zip
IN THIS ISSUE the new staff, as facilities make the contents into the plasma, is the major
flawed assumption that sticking
cause for specimen rejection as shown
FEATURE: patients to obtain blood is a simple
BD Vacutainer Systems, Preanalytical Solutions by the College of American Pathologists
The Hemolyzed procedure. In fact, a great deal of
LabNotes
First Name
Fax
alleviate the quality issues associated
(Check all that apply)
Legislative Alert:
Winter 2003
Plastic UA Preservative Tube Needleless errors, with the blame usually placed
Street Address
Last Name
Blood Transfer
on the medical technologists. Rarely is
Job Title
Clinical Laboratory
E-mail
Phone
City
me e-mail updates
products, services
receiving LabNotes
Whats New at
and promotions.
related to blood
information
Would you be
LabNotes
Results from all laboratory disciplines
can be affected by hemolysis, especially BD Vacutainer Max/Min Fill Line
Fill lines help the healthcare worker
in chemistry. Some of the more
routine tests involved are: potassium,
Plus Plastic UA maintain the correct urine-to-additive ratio
Min fill is 7mL
LAB NOTES
BD Vacutainer Systems, Preanalytical Solutions, 1 Becton Drive MC325, varying degrees of hemolysis.5
needles that are too fine (higher connection. preventing the cells from hitting the
Franklin Lakes, NJ 07417-1885.
2 3
continued from page 1
T O O L K I T : U r i n a l y s i s
appears rosy to bright red in color.
LabNotes
Results from all laboratory disciplines
can be affected by hemolysis, especially BD Vacutainer Max/Min Fill Line
Fill lines help the healthcare worker
in chemistry. Some of the more
routine tests involved are: potassium,
Plus Plastic UA maintain the correct urine-to-additive ratio
Min fill is 7mL
LAB NOTES
BD Vacutainer Systems, Preanalytical Solutions, 1 Becton Drive MC325, varying degrees of hemolysis.5
needles that are too fine (higher connection. preventing the cells from hitting the
Franklin Lakes, NJ 07417-1885.
2 3
tubes are designed to fill part way continued from page 4 of the control of the phlebotomist, References:
1. Jones BA, Calam RR and Howanitz PJ, Chemistry specimen
while maintaining the proper the above recommendations, if acceptability: a College of American Pathologists Q-Probe
too high or too low can rupture red study of 453 labs, Arch Pathol Lab Med 1997;121:19-26.
blood-to-additive ratio. The smaller followed, should greatly decrease 2. Yucel D and Dalva K, Effect of in vitro hemolysis on 25
cell membranes. Check to make sure common biochemical tests, Clin Chem 1992;38:575-577.
volume of blood drawn into partial the incidence of hemolyzed specimens 3. Burns ER and Yoshikawa N, Hemolysis in serum samples
that centrifuge temperatures are drawn by emergency department personnel versus labora-
draw tubes satisfies the CAP in the laboratory. tory phlebotomists, Lab Med 2002;33:378-380.
acceptable. Abide by the recommend- 4. Lemery LD, Oh, No! Its hemolyzed! What, why, who,
recommendation for minimizing large Consistent quality specimens can how?, Advance for Med Lab Prof. 1998;24-25.
ed transport and storage temperatures
blood draw volumes (Checklist only result from proper training and
5. Chan A, Chung H, Cockram C and Swaminathan R,
Handling of Blood Specimens for Glucose Analysis,
specified by the laboratory that is per- J. Clin. Chem. Clin. Biochem 1990;28:185-186.
Question: 01:40500), and mitigates knowledge of the factors that can
forming the assays. 6. BD White Paper VS5391, Evaluation of Sample Quality
safety concerns as less blood is influence lab results. The bottom line
and Analytic Results Between Specimens collected in
BD Vacutainer Tubes and Current Syringe Collections
handled and discarded. (Note: Partial is to obtain accurate test results that
(available upon request from BD).
Quality Specimens, 7. Carraro P, Servidio G and Plabani M, Hemolyzed
draw tubes should not be confused Quality Test Results truly reflect the patients status. To
Specimens; A reason for rejection or a clinical chanllenge?,
Clin Chem[Letter]2000;46:306-7.
with small size pediatric tubes that ensure that this happens, facilities
8. Kennedy C, Angenmuller S, King R, Noviello S, Walker J,
Warden J and Vang S, A comparison of hemolysis rates
are fully evacuated.) Proper specimen collection and using intravenous catheters versus venipuncture tubes for
should establish standard protocols obtaining blood samples, J Emerg Nurs. 1996;22(6):566-69.
handling techniques are critical to 9. Savory J, Bill J, Hemolysis of Specimens Drawn in the ER,
Specimen Handling Techniques for specimen collection, and make Lab Med [Q&A] 1996;Vol27,No.12:802.
producing quality laboratory results. 10. Sixsmith DM, Weinbaum F, Chan SYA, Nussabaum M,
certain that the proper training and Magdich K, Reduction of Hemolysis of Blood Specimens
Although there will occasionally be Drawn from ED Patients for Routine Chemistry Tests by
Once the proper specimen collection expertise are in place with each Use of Low Vacuum Collection Tubes, Acad Emerg
sources of hemolysis that are out Med[Abstract] 2000;vol 7, No.5: 524.
techniques are applied, subsequent potential phlebotomist.
specimen handling factors must be
considered to prevent hemolysis from
continued from page 3
The high degree of variability
occurring in the pre-analytical phase. Legislative Alert
bottom of the tube with such a great in the training, skills, and
Consider the factors listed below to Needleless Blood Transfer
force, RBC trauma can be reduced. frequency of phlebotomy prevent hemolysis:
practice of the non-laboratory Mixing TubesMix the blood with
Peripheral Catheter CollectionsThe
staff is a major factor in the tube additive through gentle tube ... If drawing blood with a syringe is necessary,
highest rates of hemolyzed specimens the increase of hemolysis
appear to come from the acute care inversions. Do not shake the tube engineering controls (engineered sharps injury
rates in many facilities.
setting, i.e., Emergency Dept (ED), after collection. protection) and safe work practices (including
Labor and Delivery (L&D), and Transport MethodsBe cautious with mechanical means of removal if available) must
Intensive Care Units (ICU).3 Studies compared to no hemolysis at all in the pneumatic tube systems and other be used and needleless blood transfer
have shown that the main source [peripheral] venipuncture specimens.9 rough transport conditions that can devices must be implemented.
Our Clinical Lab Scholarship program is one of the many
of hemolysis in the ER is the use of Blood collected from the back create turbulence and RBC trauma ways that BD Vacutainer, Preanalytical Solutions supports Occupational Safety & Health Administration (OSHA),
IV catheters for specimen collection. within the tube. Hand deliver speci- US Department of Labor
of the IV catheter is pulled through the continuing education of medical professionals.
June 12, 2002 OSHA Standard Interpretation
One study found that specimens several gauges; the catheter generally mens when feasible. Specimens should Each year, scholarships are awarded to individuals from letter on the Re-use of blood tube holders.
drawn by nurses though an IV ranges from 18 to 22G, the Luer be stored in an upright position fol- laboratories and hospitals across the United States.
catheter were more than 3 times as adapter front end is 15G, and lowing centrifugation.
Our upcoming scholarship program will grant each
likely to be hemolyzed than those the stopper-piercing needle is 20G. Rimming clotsDo not use wooden recipient a trip to the 2003 CLMA/ASCP Conference, to
drawn by venipuncture (13.7% vs Slowing down this pull rate can applicator sticks to rim clots, which be held June 21-24, 2003 in Salt Lake City. Scholarship
3.8%).8 In another study conducted at reduce the hemolysis rate significantly. can shred the red cells. With the cur- recipients are also invited to attend an exclusive dinner
the University of Virginia Health The use of partial draw collection rent evacuated serum tubes available, meeting with keynote addresses by prominent clinicians.
Sciences Center, specimens were tubes is an effective way to slow down rimming clots is unnecessary.
collected using IV catheters and the pressure exerted on the blood Visit our website at www.bd.com/vacutainer/scholarship Reference
TemperatureStore and transport #364880
venipuncture, and then compared. and, thus reduce hemolysis.10 The for additional information on this exciting scholarship
specimens in regulated temperature
The results were striking, revealing program and for information on when to enter. BD Vacutainer
LAB NOTES
reduced vacuum in these tubes yields
conditions, as temperatures that are
a 50% rate of hemolysis in the a slower, gentler draw. Partial draw Blood Transfer Device
IV catheter collected specimens, continued on page 5
4 5
tubes are designed to fill part way continued from page 4 of the control of the phlebotomist, References:
1. Jones BA, Calam RR and Howanitz PJ, Chemistry specimen
while maintaining the proper the above recommendations, if acceptability: a College of American Pathologists Q-Probe
too high or too low can rupture red study of 453 labs, Arch Pathol Lab Med 1997;121:19-26.
blood-to-additive ratio. The smaller followed, should greatly decrease 2. Yucel D and Dalva K, Effect of in vitro hemolysis on 25
cell membranes. Check to make sure common biochemical tests, Clin Chem 1992;38:575-577.
volume of blood drawn into partial the incidence of hemolyzed specimens 3. Burns ER and Yoshikawa N, Hemolysis in serum samples
that centrifuge temperatures are drawn by emergency department personnel versus labora-
draw tubes satisfies the CAP in the laboratory. tory phlebotomists, Lab Med 2002;33:378-380.
acceptable. Abide by the recommend- 4. Lemery LD, Oh, No! Its hemolyzed! What, why, who,
recommendation for minimizing large Consistent quality specimens can how?, Advance for Med Lab Prof. 1998;24-25.
ed transport and storage temperatures
blood draw volumes (Checklist only result from proper training and
5. Chan A, Chung H, Cockram C and Swaminathan R,
Handling of Blood Specimens for Glucose Analysis,
specified by the laboratory that is per- J. Clin. Chem. Clin. Biochem 1990;28:185-186.
Question: 01:40500), and mitigates knowledge of the factors that can
forming the assays. 6. BD White Paper VS5391, Evaluation of Sample Quality
safety concerns as less blood is influence lab results. The bottom line
and Analytic Results Between Specimens collected in
BD Vacutainer Tubes and Current Syringe Collections
handled and discarded. (Note: Partial is to obtain accurate test results that
(available upon request from BD).
Quality Specimens, 7. Carraro P, Servidio G and Plabani M, Hemolyzed
draw tubes should not be confused Quality Test Results truly reflect the patients status. To
Specimens; A reason for rejection or a clinical chanllenge?,
Clin Chem[Letter]2000;46:306-7.
with small size pediatric tubes that ensure that this happens, facilities
8. Kennedy C, Angenmuller S, King R, Noviello S, Walker J,
Warden J and Vang S, A comparison of hemolysis rates
are fully evacuated.) Proper specimen collection and using intravenous catheters versus venipuncture tubes for
should establish standard protocols obtaining blood samples, J Emerg Nurs. 1996;22(6):566-69.
handling techniques are critical to 9. Savory J, Bill J, Hemolysis of Specimens Drawn in the ER,
Specimen Handling Techniques for specimen collection, and make Lab Med [Q&A] 1996;Vol27,No.12:802.
producing quality laboratory results. 10. Sixsmith DM, Weinbaum F, Chan SYA, Nussabaum M,
certain that the proper training and Magdich K, Reduction of Hemolysis of Blood Specimens
Although there will occasionally be Drawn from ED Patients for Routine Chemistry Tests by
Once the proper specimen collection expertise are in place with each Use of Low Vacuum Collection Tubes, Acad Emerg
sources of hemolysis that are out Med[Abstract] 2000;vol 7, No.5: 524.
techniques are applied, subsequent potential phlebotomist.
specimen handling factors must be
considered to prevent hemolysis from
continued from page 3
The high degree of variability
occurring in the pre-analytical phase. Legislative Alert
bottom of the tube with such a great in the training, skills, and
Consider the factors listed below to Needleless Blood Transfer
force, RBC trauma can be reduced. frequency of phlebotomy prevent hemolysis:
practice of the non-laboratory Mixing TubesMix the blood with
Peripheral Catheter CollectionsThe
staff is a major factor in the tube additive through gentle tube ... If drawing blood with a syringe is necessary,
highest rates of hemolyzed specimens the increase of hemolysis
appear to come from the acute care inversions. Do not shake the tube engineering controls (engineered sharps injury
rates in many facilities.
setting, i.e., Emergency Dept (ED), after collection. protection) and safe work practices (including
Labor and Delivery (L&D), and Transport MethodsBe cautious with mechanical means of removal if available) must
Intensive Care Units (ICU).3 Studies compared to no hemolysis at all in the pneumatic tube systems and other be used and needleless blood transfer
have shown that the main source [peripheral] venipuncture specimens.9 rough transport conditions that can devices must be implemented.
Our Clinical Lab Scholarship program is one of the many
of hemolysis in the ER is the use of Blood collected from the back create turbulence and RBC trauma ways that BD Vacutainer, Preanalytical Solutions supports Occupational Safety & Health Administration (OSHA),
IV catheters for specimen collection. within the tube. Hand deliver speci- US Department of Labor
of the IV catheter is pulled through the continuing education of medical professionals.
June 12, 2002 OSHA Standard Interpretation
One study found that specimens several gauges; the catheter generally mens when feasible. Specimens should Each year, scholarships are awarded to individuals from letter on the Re-use of blood tube holders.
drawn by nurses though an IV ranges from 18 to 22G, the Luer be stored in an upright position fol- laboratories and hospitals across the United States.
catheter were more than 3 times as adapter front end is 15G, and lowing centrifugation.
Our upcoming scholarship program will grant each
likely to be hemolyzed than those the stopper-piercing needle is 20G. Rimming clotsDo not use wooden recipient a trip to the 2003 CLMA/ASCP Conference, to
drawn by venipuncture (13.7% vs Slowing down this pull rate can applicator sticks to rim clots, which be held June 21-24, 2003 in Salt Lake City. Scholarship
3.8%).8 In another study conducted at reduce the hemolysis rate significantly. can shred the red cells. With the cur- recipients are also invited to attend an exclusive dinner
the University of Virginia Health The use of partial draw collection rent evacuated serum tubes available, meeting with keynote addresses by prominent clinicians.
Sciences Center, specimens were tubes is an effective way to slow down rimming clots is unnecessary.
collected using IV catheters and the pressure exerted on the blood Visit our website at www.bd.com/vacutainer/scholarship Reference
TemperatureStore and transport #364880
venipuncture, and then compared. and, thus reduce hemolysis.10 The for additional information on this exciting scholarship
specimens in regulated temperature
The results were striking, revealing program and for information on when to enter. BD Vacutainer
LAB NOTES
reduced vacuum in these tubes yields
conditions, as temperatures that are
a 50% rate of hemolysis in the a slower, gentler draw. Partial draw Blood Transfer Device
IV catheter collected specimens, continued on page 5
4 5
Understanding Additives: You can place your trust in BD Vacutainer
Systems, Preanalytical Solutions to
EDTA provide meaningful opportunities for
RELATED INDUSTRY WEBSITE educational learning experiences. Another
E thylenediaminetetraacetic acid
(EDTA) salts are often used as Whats New at NCCLS?
www.nccls.org valuable service provided to you from BD.
FP
This document provides guidance for conducting molecular tests of immunoglobulin and
concentrations (11% shrinkage comparing K3EDTA glass tubes but the NCCLS forum might T-cell receptor gene arrangements.
with 7.5 mg/mL blood). to K2EDTA plastic tubes.5,6 take other forms, such as a
conference, a set of consensus MM6-P Quantitative Molecular Methods for Infectious Diseases; Proposed Guideline
K3EDTA produces a larger increase The International Council EDTA is used as the anticoagulant This document provides guidance for the development and use of quantitative molecular
for Standardization in recommendations, general
in cell volume on standing (1.6% of choice in blood collections for com- methods, such as nucleic acid probes and nucleic acid amplification techniques of the target
Haematology and NCCLS have information, an educational
increase after 4 hours). plete blood counts (CBC), microhema- sequences specific to particular microorganisms. It also presents recommendations for quality
O
recommended K2EDTA as the workshop, or a video.
K3EDTA leads to lower MCV tocrits (packed cell volume [PCV]), assurance, proficiency testing and interpretation of results.
anticoagulant of choice for differential leukocyte counts, platelet
values (typically a -0.1 to -1.3%
blood cell counting and sizing.
LAB NOTES
difference is observed compared counts, reticulocyte counts and flow
NO POSTAGE
NECESSARY
with K2EDTA). continued on back cover
6 7
Understanding Additives: You can place your trust in BD Vacutainer
Systems, Preanalytical Solutions to
EDTA provide meaningful opportunities for
RELATED INDUSTRY WEBSITE educational learning experiences. Another
E thylenediaminetetraacetic acid
(EDTA) salts are often used as Whats New at NCCLS?
www.nccls.org valuable service provided to you from BD.
FP
This document provides guidance for conducting molecular tests of immunoglobulin and
concentrations (11% shrinkage comparing K3EDTA glass tubes but the NCCLS forum might T-cell receptor gene arrangements.
with 7.5 mg/mL blood). to K2EDTA plastic tubes.5,6 take other forms, such as a
conference, a set of consensus MM6-P Quantitative Molecular Methods for Infectious Diseases; Proposed Guideline
K3EDTA produces a larger increase The International Council EDTA is used as the anticoagulant This document provides guidance for the development and use of quantitative molecular
for Standardization in recommendations, general
in cell volume on standing (1.6% of choice in blood collections for com- methods, such as nucleic acid probes and nucleic acid amplification techniques of the target
Haematology and NCCLS have information, an educational
increase after 4 hours). plete blood counts (CBC), microhema- sequences specific to particular microorganisms. It also presents recommendations for quality
O
recommended K2EDTA as the workshop, or a video.
K3EDTA leads to lower MCV tocrits (packed cell volume [PCV]), assurance, proficiency testing and interpretation of results.
anticoagulant of choice for differential leukocyte counts, platelet
values (typically a -0.1 to -1.3%
blood cell counting and sizing.
LAB NOTES
difference is observed compared counts, reticulocyte counts and flow
NO POSTAGE
NECESSARY
with K2EDTA). continued on back cover
6 7
LabNotes a newsletter from BD Vacutainer Systems
Trust BD Vacutainer
DATED MATERIAL
Family of products you can rely on
Please Open
VERSION #3 continued from page 7
complimentary issue of LabNotes. kinase and leucine aminopeptidase mixing and proper anticoagulation
1985;109;247-249.
4. Brunson D. Smith, Bak A, Przyk E, Sheridan B, Muncer DL,
Comparing Hematology Anticoagulants; K2EDTA vs
K3EDTA. Lab Hematology 1995; 1:112-119.
Systems, The Hemolyzed Specimen:
determinations. of the specimen. Preanalytical
Causes, Effects, and Reduction
5. VS5244 (white paper), BD Vacutainer Tube Comparison:
To continue receiving free copies of this newsletter, It is important that the proper
Plastic K2EDTA vs. Glass K3EDTA Tubes for Blood Counts
on the Coulter MAXM. Solutions
please send us this postage-paid reply card and we will amount of blood be drawn into EDTA
6. VS5324 (white paper), BD Vacutainer Plus Tube
13 x100mm with Spray-Coated K2EDTA; An Evaluation of
gladly add you to our subscriber list. Visual Hemolysis, Foaming, Clotting and Selected
tubes, with the caution to avoid short Hematology Parameters.
draws. An excess of EDTA in the Our internal studies showed no By Valerie Bush, PhD. The issue of hemolysis has always The high degree of variability in
Lynn Mangan, MT, (ASCP)
01/03 VS7044
blood may increase osmotic pressure plagued clinical laboratories and the training, skills, and frequency
clinically significant differences See
and distort or rupture cells. Excess when comparing K3EDTA glass For In Inside continues to be a growing concern. of phlebotomy practice of the non-
On Ho structions In many hospitals, nurses and other
Your feedback is appreciated!
EDTA causes shrinkage of red cells tubes to K2EDTA plastic tubes. w To laboratory staff is a major factor in
healthcare workers have replaced
Your F Obtain the increase of hemolysis rates in many
We welcome your comments. Please describe specific subject matter that you
YES, I would like a complimentary subscription to
crit, mean corpuscular volume (MCV) cripti phlebotomists. Often this decentral- facilities. Hemolysis, defined as red
# of Beds
on
ization, as it is called, occurs with blood cell breakdown and the release
MI
Zip
IN THIS ISSUE the new staff, as facilities make the contents into the plasma, is the major
flawed assumption that sticking
cause for specimen rejection as shown
FEATURE: patients to obtain blood is a simple
BD Vacutainer Systems, Preanalytical Solutions by the College of American Pathologists
The Hemolyzed procedure. In fact, a great deal of
LabNotes
First Name
Fax
alleviate the quality issues associated
(Check all that apply)
Legislative Alert:
Winter 2003
Plastic UA Preservative Tube Needleless errors, with the blame usually placed
Street Address
Last Name
Blood Transfer
on the medical technologists. Rarely is
Job Title
Clinical Laboratory
E-mail
Phone
City
me e-mail updates
products, services
receiving LabNotes
Whats New at
and promotions.
related to blood
information
Would you be
complimentary issue of LabNotes. kinase and leucine aminopeptidase mixing and proper anticoagulation
1985;109;247-249.
4. Brunson D. Smith, Bak A, Przyk E, Sheridan B, Muncer DL,
Comparing Hematology Anticoagulants; K2EDTA vs
K3EDTA. Lab Hematology 1995; 1:112-119.
Systems, The Hemolyzed Specimen:
determinations. of the specimen. Preanalytical
Causes, Effects, and Reduction
5. VS5244 (white paper), BD Vacutainer Tube Comparison:
To continue receiving free copies of this newsletter, It is important that the proper
Plastic K2EDTA vs. Glass K3EDTA Tubes for Blood Counts
on the Coulter MAXM. Solutions
please send us this postage-paid reply card and we will amount of blood be drawn into EDTA
6. VS5324 (white paper), BD Vacutainer Plus Tube
13 x100mm with Spray-Coated K2EDTA; An Evaluation of
gladly add you to our subscriber list. Visual Hemolysis, Foaming, Clotting and Selected
tubes, with the caution to avoid short Hematology Parameters.
draws. An excess of EDTA in the Our internal studies showed no By Valerie Bush, PhD. The issue of hemolysis has always The high degree of variability in
Lynn Mangan, MT, (ASCP)
01/03 VS7044
blood may increase osmotic pressure plagued clinical laboratories and the training, skills, and frequency
clinically significant differences See
and distort or rupture cells. Excess when comparing K3EDTA glass For In Inside continues to be a growing concern. of phlebotomy practice of the non-
On Ho structions In many hospitals, nurses and other
Your feedback is appreciated!
EDTA causes shrinkage of red cells tubes to K2EDTA plastic tubes. w To laboratory staff is a major factor in
healthcare workers have replaced
Your F Obtain the increase of hemolysis rates in many
We welcome your comments. Please describe specific subject matter that you
YES, I would like a complimentary subscription to
crit, mean corpuscular volume (MCV) cripti phlebotomists. Often this decentral- facilities. Hemolysis, defined as red
# of Beds
on
ization, as it is called, occurs with blood cell breakdown and the release
MI
Zip
IN THIS ISSUE the new staff, as facilities make the contents into the plasma, is the major
flawed assumption that sticking
cause for specimen rejection as shown
FEATURE: patients to obtain blood is a simple
BD Vacutainer Systems, Preanalytical Solutions by the College of American Pathologists
The Hemolyzed procedure. In fact, a great deal of
LabNotes
First Name
Fax
alleviate the quality issues associated
(Check all that apply)
Legislative Alert:
Winter 2003
Plastic UA Preservative Tube Needleless errors, with the blame usually placed
Street Address
Last Name
Blood Transfer
on the medical technologists. Rarely is
Job Title
Clinical Laboratory
E-mail
Phone
City
me e-mail updates
products, services
receiving LabNotes
Whats New at
and promotions.
related to blood
information
Would you be
FP
This document provides guidance for conducting molecular tests of immunoglobulin and
concentrations (11% shrinkage comparing K3EDTA glass tubes but the NCCLS forum might T-cell receptor gene arrangements.
with 7.5 mg/mL blood). to K2EDTA plastic tubes.5,6 take other forms, such as a
conference, a set of consensus MM6-P Quantitative Molecular Methods for Infectious Diseases; Proposed Guideline
K3EDTA produces a larger increase The International Council EDTA is used as the anticoagulant This document provides guidance for the development and use of quantitative molecular
for Standardization in recommendations, general
in cell volume on standing (1.6% of choice in blood collections for com- methods, such as nucleic acid probes and nucleic acid amplification techniques of the target
Haematology and NCCLS have information, an educational
increase after 4 hours). plete blood counts (CBC), microhema- sequences specific to particular microorganisms. It also presents recommendations for quality
O
recommended K2EDTA as the workshop, or a video.
K3EDTA leads to lower MCV tocrits (packed cell volume [PCV]), assurance, proficiency testing and interpretation of results.
anticoagulant of choice for differential leukocyte counts, platelet
values (typically a -0.1 to -1.3%
blood cell counting and sizing.
LAB NOTES
difference is observed compared counts, reticulocyte counts and flow
NO POSTAGE
NECESSARY
with K2EDTA). continued on back cover
6 7