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Abstract The flavonoids contained in Euphorbia neriifolia leaves were extracted, identified and

characterized. Direct and sequential soxhlet extraction and its concentrated fractions were subjected

to thin layer chromatography and high performance thin layer chromatography. The results

showed that maximum yield of the flavonoid (6.53 g) was obtained from ethanolic extract. The

R f value of isolated flavonoid and phytochemical screening has been compared with standard

Quercetin. Characterization of isolated flavonoid was done by IR,

1 H NMR, and MS. On the basis

of chemical and spectral analysis structure was elucidated as 2-(3,4-dihydroxy-5-methoxy-phenyl)-

3,5-dihydroxy-6,7-dimethoxychromen-4-one, a flavonoid. This compound was isolated for the first

time from this plant. hgghfghfderse

2014 Production and hosting by Elsevier B.V. on behalf of King Saud University. Thisisanopenaccess

article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

1. Introduction

Phytochemistry reports thousands of newer organic molecules

or compounds every year. Pharmacological testing, modifying,

derivatizing and research on these natural products represent a

major strategy for discovering and developing new drugs

(Kayser et al., 2000). Moreover, plant secondary metabolites

present chemical and pharmaceutical properties interesting

for human hea