Determination of Neomycin sulphate

Method VPH0004J
HPLC RP Mode

Column: ProntoSil 120-5 C18 AQ, 250 x 4.0 mm ID Order No. 25WF184PSJ

Phase: ProntoSil 120-5 C18 AQ

Conditions: Eluent: 170 mM TFA in H2O

Gradient: isocratic
Flow rate: 0.7 ml/min
Temperature: 30 C
Volume: 50 l

Detection: ELSD, 50 C, 4 bar nitrogen

Substances: Neomycin sulphate B, Neomycin sulphate C

Keywords: Neomycin sulphate, aminoglycoside antibiotics, ELSD

Chromatogram:

acidic sample solution

(ointment)

1 Imp. 1
2 Imp. 2
3 Imp. 3
4 Neomycin sulphate C
5 Neomycin sulphate B
5
1
100
4
75
Neom ycin C

2
mV

50
Neom ycin B

25 3

0 1 2 3 4 5 6 7 8 9 10
Minutes