Time OD Dilution Final Absorbance HA OD dilution factor 0

0 0.019 1 0.019 0.283 0.177 5 0.885 0.219

5 0.222 10 2.22 0.46 0.163 5 0.815 0.201
6 0.308 10 3.08 0.446 0.341 5 1.705 0.421
7 0.492 10 4.92 0.624 0.345 5 1.725 0.426
8 0.494 10 4.94 0.628 0.483 5 2.415 0.597
9 0.836 10 8.36 0.766 0.379 5 1.895 0.468
10 0.638 20 12.76 0.662 0.359 5 1.795 0.443
11 0.499 20 9.98 0.442 0.358 5 1.79 0.442
12 0.49 20 9.8 0.441 0.322 5 1.61 0.398
14 0.51 20 10.2 0.505 0.318 5 1.59 0.393
17.5 0.354 50 17.7 0.469 0.288 5 1.44 0.356
21.5 0.42 50 21 0.571 5

HA Concentration
0.7

0.6

0.5

0.4 H

0.3

0.2

0.1

0
0 5 10 15 20 25
 Growth Curve
25

20

15 Growth Curve

10

0
0 5 10 15 20 25

Concentration

HA Concentration

15 20 25