IAETSD JOURNAL FOR ADVANCED RESEARCH IN APPLIED SCIENCES ISSN NO: 2394-8442

Action of Photo Activated Cow Urine with the Different Combination of

Neem and Aloe Vera Extracts against Pathogenic Microorganisms
1
Naveen Sharma, 2 Jagdamba Prasad, 3 Kamal Jaiswal
1 2 3 Department of Biotechnology, Saroj Institute of Technology and Management, Lucknow (U.P.), India
adi13sept@gmail.com

Abstract: From the ancient period cow’s urine has been used as a medicine. In India, drinking of cow urine has been
practiced for thousands of years. The present study analyses the antibacterial and antifungal activity of photo activated Cow
Urine with the combination of neem and aloe-vera against the clinical pathogenic microorganisms. Antibacterial activity of
photo activated Cow Urine was analysed against the Escherichia coli and Salmonella typhi. Maximum antibacterial activity was
observed in Salmonella typhi (10.2 mm in diameter) than Escherichia coli (7.2 mm in diameter). Antifungal activity of photo
activated cow urine was analysed against Aspergillus niger and Penicillium crysogenum. When the two fungal organisms were
compared, maximum growth suppression was observed in Aspergillus niger (12.8 mm in diameter) than Penicillium crysogenum
(10.2 mm in diameter).Finally concluded that the photo activated cow urine with the combination of different extracts has
antibacterial and antifungal activities and the inhibitory activity can be used in the control of bacteria and fungi of various
origins.

Key words: photo activated cow urine, antibacterial, antifungal, neem and aloe-vera.

INTRODUCTION
In Veda, cow’s urine was compared to the nectar. In substrata, several medicinal properties of cow urine have been
mentioned and are known to cause weight loss, reversal of certain cardiac and kidney problems, indigestion, stomach ache,
edema, etc. Cow urine has a unique place in Ayurveda and has been described in ‘SushritaSumhita’ and AshtangaSangraha’
to be the most effective substance secretion of animal origin with innumerable therapeutic values. It has been recognized as
water of life or “Amrita” (Beverages of immortality), the nectar of the God. In India, drinking of cow urine has been practiced
for thousands of years. Panchagavya is a term used in Ayurveda to describe five important substances obtained from cow
namely Urine, Dung, Milk, Ghee and Curd. A number of formulations mentioned in Ayurveda describe the use of
Panchagavya components either alone or in combination with drugs of herbal, animal or mineral origin [1].

An exhaustive reference of cow’s urine having curative properties in skin diseases, especially leprosy, is referred to in
Carakasamhita [2].The photo activated cow urine has been patented as activity enhancer and availability facilitator for
bioactive molecules including anti- infective and anti-cancer agents (US Patent No 6410 059/2002) [3]. Chakra panimishra
in vishvavallabba recommends using extracts of cow urine in herbal insecticides [4]. Feeding of cow urine increased the feed
intake in white leg born layers [5]. The present study was carried out to prepare photo activated cow urine and to determine
the antibacterial and antifungal activities.

MATERIALS AND METHODS

Collection of Sample: cow urine was collected from cow farm using sterile container. For experiments fresh cow urine was
photo activated for (144 h) in sunlight by keeping it in sealed glass bottles .Then cow urine was stored at 4°C for long-term
use[6].

VOLUME 4, ISSUE 7, DEC/2017 231 http://iaetsdjaras.org/

IAETSD JOURNAL FOR ADVANCED RESEARCH IN APPLIED SCIENCES ISSN NO: 2394-8442

Preparation of Plant Extracts: 20 g of each plant sample was crushed and was placed in 200 ml of distilled water which
was then placed on rotary shaker for overnight. It was then filtered with filter paper (Whattmann no.1). The solvent was
evaporated using Rotary vacuum shaker to concentrate the solution. The obtained extract was stored in vials.

Sub Culturing of Microorganisms: Microorganisms namely Escherichia coli, Salmonella typhi, Aspergillus niger, Penicillium
crysogenum were sub-cultured from pure culture in nutrient broth.

Antibacterial and Antifungal Activity of Photo Activated Cow Urine: The antimicrobial and antifungal activity studies
were carried out by agar well diffusion technique [7]. The sterile Mueller Hinton agar plates were prepared. The test
organisms like Escherichia coli, Salmonella typhi, Aspergillus niger and penicillium crysogenum were spreaded over the Mueller Hinton
ager plates by using separate sterile cotton swabs. After the spreading, wells were prepared of 6 mm diameter, then
100μlphoto activated cow urine with the combination of different extracts of neem and aloe vera were filled in the wells. All
the bacterial plates were incubated at 37°C for 24hrs and fungal plates at 24°C for 72 hrs. After incubation the diameter of
the minimum zone of inhibition was measured in mm.

RESULTS AND DISCUSSIONS

Antibacterial Activity: Antibacterial activity of photo activated cow urine was analysed along with different combination
of neem and aloe vera against Escherichia coli and Salmonella typhi(Table 1, Fig. 1 and Fig. 3).Maximum antibacterial activity
was observed in Salmonella typhi (10.2, 9.7, 8.8mm in diameter respectively) than Escherichia coli (7.2, 6, 5.2mm in diameter
respectively) and the standard antibiotic (Ampicillin).

Antifungal Activity: Antifungal activity of photo activated cow urine was analysed against Aspergillus niger and Penicillium
crysogenum. The investigated results were presented in Table 1, Fig. 2 and Fig. 3. When the two fungal organisms were
compared, maximum growth suppression was observed in Aspergillus niger (12.8, 11.7 and 11 mm in diameter, respectively)
than Penicillium crysogenum (10.2, 8.8 and 6.3, mm in diameter, respectively).

S.NO. Microorganisms Sample Zone of Inhibition of Aqueous

Extract
(dia. in mm)
1 E. coli Antibiotic 8.1
PCU+ Neem 7.2
PCU+ Aloe Vera 6
Cow Urine 5.2
2 Salmonella typhi Antibiotic 11
PCU+ Neem 10.2
PCU+ Aloe Vera 9.7
Cow Urine 8.8
3 Aspergillus niger Antibiotic 14.5
PCU+ Neem 12.8
PCU+ Aloe Vera 11.7
Cow Urine 11
4 Penicilliumcrysogenum Antibiotic 11
PCU+ Neem 10.2
PCU+ Aloe Vera 8.8
Cow Urine 6.3
Table 1: Representing different microorganism, samples and inhibition zone (mm)

VOLUME 4, ISSUE 7, DEC/2017 232 http://iaetsdjaras.org/

IAETSD JOURNAL FOR ADVANCED RESEARCH IN APPLIED SCIENCES ISSN NO: 2394-8442

12

10

8
Antibiotic
6
PCU+Neem
PCU+Aloe vera
4
Cow Urine
2

0
E. coli S. typhi

Fig. 1: Antibacterial Activity of Photo Activated Cow Urine

X axis: Microorganisms
Y axis: Zone of inhibition (diameter in mm)

16

14

12

10
Antibiotic
8 PCU+Neem

6 PCU+Aloe vera
Cow Urine
4

0
Aspergillus niger penicillum
crysogenum
Fig.2: Antifungal Activity of Photo Activated Cow Urine
X axis: Microorganisms
Y axis: Zone of Inhibition (diameter in mm)

Fig.3: Antibacterial Activity of Photo Activated Cow Urine

VOLUME 4, ISSUE 7, DEC/2017 233 http://iaetsdjaras.org/

IAETSD JOURNAL FOR ADVANCED RESEARCH IN APPLIED SCIENCES ISSN NO: 2394-8442

Fig.4: Antifungal Activity of Photo Activated Cow Urine

CONCLUSSION & FUTURE PROSPECTS

It was concluded that the photo activated cow urine has antibacterial and antifungal inhibitory activity, can be used in the
control of bacteria and fungi of various origins. The test was done in vitro. Now a day’s urine therapy treatment was
developed in various medical sectors. It is most effective natural remedy and safest method of treatment without any side
effects. It can prevent, control and cure all kinds of chronic diseases such as cancer, diabetes, blood pressure HIV/AIDS ,
kidney failure arthritis ,hair loss etc. it can boost the immune system, improve nervous disorder, dissolves and removes
toxins which are accumulated in our body. It can revive dead tissues, and rebuild resistant power of vital organs like brain,
heart, lungs, liver intestine etc.

REFERENCES
1. Shah, E., 1997. Herbal composition in cow urine distillate. US 5693327. Dec 2.
2. Basham, A.L., 1998. Practices of medicine in ancient India in Asian Medical systems: A Comparative study. (Ed) by Charles leslie, Motilal
Banarsidass, Delhi, pp: 22.
3. Sarman Singh, 2001. Cow urine has anti Leshmania donovani effect in vitro. International J. Cow Sci.,1(2): 72-73.
4. Sadhale, N., 2004. Vishvallabba.Agri History Bulletin 5. Asian agri-History Foundation, Secunderabad. 500009. pp: 134.
5. Garg, N., Ashok Kumar and R.S.Chauhan, 2005.Effect of indigenous cow urine on nutrient utilization of white leghorn layers. International
J. Cow Sci., 1: 36-38.
6. Khanuja, S.P.S., 2002. Pharmaceutical composition containing cow urine distillate and an Antibiotic.US patent 6410059. June 25.
7. Bauer, R.W., M.D.K. Kirby, J.C. Sherris and M. Turck, 1966. Antibiotic susceptibility testing by standard single disc diffusion method.
American J. Clinical Pathol., 45: 493-496.

VOLUME 4, ISSUE 7, DEC/2017 234 http://iaetsdjaras.org/