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Journal of the Saudi Society of Agricultural Sciences xxx (2017) xxx–xxx

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Technological and nutritional properties of gluten-free snacks based on


brown rice and chestnut flour
Shabir Ahmad Mir a,b,⇑, Sowriappan John Don Bosco b, Manzoor Ahmad Shah b
a
Department of Food Technology, Islamic University of Science and Technology, Awantipora, Jammu and Kashmir 192122, India
b
Department of Food Science and Technology, Pondicherry University, Puducherry 605014, India

a r t i c l e i n f o a b s t r a c t

Article history: Effect of different ratios of chestnut flour substitution on brown rice flour based snack was investigated
Received 8 January 2017 for technological and nutritional properties. Pasting properties significantly varied among the flour ratios
Revised 25 February 2017 and the higher levels of chestnut flour led to the increase in pasting properties. Colour parameters, L⁄, a⁄
Accepted 28 February 2017
and b⁄ values of snacks decreased with increase in chestnut flour level. Expansion ratio of snacks is
Available online xxxx
increased while as hardness decreased with progressive increase of chestnut flour. Mineral analysis
showed that snacks were rich in potassium, phosphorus, sulphur and magnesium etc. Scanning electronic
Keywords:
microscopy showed the porous structure of fried snack which leads to increase in expansion ratio.
Snacks
Brown rice
Flavour value of snacks was increased with the increase in chestnut flour level in the blend. Based on pre-
Chestnut flour sent study, brown rice-chestnut flour based snacks have good potential for consumer acceptance and are
Expansion ratio regarded as health promoting functional food, especially for celiac disease patients.
Ó 2017 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an
open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

1. Introduction in the germ and bran layers, which are mostly removed by polish-
ing as a consequence; milled rice has a lower nutritional quality
The change of life style pattern and demand for convenience than brown rice (Monks et al., 2013). Brown rice is a good source
foods have increased the market for snack foods. Snack foods are of functional components which provide health benefit beyond
popular products that are highly appreciated and consumed basic nutrition (Ito et al., 2005). Human and animal studies have
throughout the world (Hossain and Shin, 2013). Different types shown that consumption of brown rice reduces the risk of type-2
of snack foods are available in market and are designed to be less diabetes, cardiovascular disease, cancer and these protective
perishable, more durable and more appealing. The new generation health effects have been linked to the presence of bioactive com-
of snacks falls into several categories such as low fat, high fat, pounds such as antioxidants, polyphenols, minerals, vitamins and
baked, fried etc. (Mazumder et al., 2007; Shittu and Olaitan, dietary fibre in the bran layer of rice grain (Kim et al., 2012; Mir
2014). Frying is commonly used method for the production of et al., 2016). Rice flour is usually preferred in the gluten-free food
snack food both commercially and at household level. Fried foods products because of its hypoallergenic property, low protein con-
are considered as concentrated sources of energy and fat and tent and is rich in easily digestible carbohydrates (Sciarini et al.,
imparting characteristic colour, texture, and flavour to the product 2010).
(Ravi and Susheelamma, 2004). Chestnut is aquatic nut cultivated in a variety of growing condi-
Nowadays, cereal based snacks have gained importance due to tions and climates, being globally popular and valued for its sen-
their nutritional value and sensory attributes (Mir et al., 2017). sory, nutritional, and health attributes. Chestnut flour is used as
Rice is the most widely consumed cereal crop for a large part of raw material in various food products due to its health benefits,
the world’s human population (Mir et al., 2013). Brown rice is com- nutritional and flavour properties (Singh et al., 2011; Yang et al.,
posed of external thin layer of bran that encloses the embryo and 2015). Chestnut flour contains high quality proteins with essential
endosperm. The nutritional components in brown rice mainly exist amino acids (4–7%), relatively high amount of sugar (20–32%),
starch (50–60%), dietary fiber (4–10%). In addition, it is also rich
in minerals especially potassium, phosphorous and magnesium
Peer review under responsibility of King Saud University. (Morrone et al., 2015). Since most of the gluten-free cereal prod-
⇑ Corresponding author at: Department of Food Technology, Islamic University of ucts do not contain sufficient amount of health beneficial compo-
Science and Technology, Awantipora, Jammu and Kashmir 192122, India.
nents, it may be advantageous to use chestnut flour due to its
E-mail address: shabirahmir@gmail.com (S.A. Mir).

http://dx.doi.org/10.1016/j.jssas.2017.02.002
1658-077X/Ó 2017 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Please cite this article in press as: Mir, S.A., et al. Technological and nutritional properties of gluten-free snacks based on brown rice and chestnut flour.
Journal of the Saudi Society of Agricultural Sciences (2017), http://dx.doi.org/10.1016/j.jssas.2017.02.002
2 S.A. Mir et al. / Journal of the Saudi Society of Agricultural Sciences xxx (2017) xxx–xxx

nutritional value. Chestnut flour used as raw materials for making and the base in load cell of the instrument was fixed at 10 mm,
different food products like bread, cookies, snacks, pasta, etc. so that when the probe moved down it would compress the test
(Singh et al., 2011; Demirkesen et al., 2010). sample kept horizontally on the base to a distance of 0.500 mm.
The literature reported that blending chestnut flour with rice program was set to move the probe at 1.0 mm/min in both pre-
flour resulted in better quality products (Sacchetti et al., 2004). test and post-test phases. A 5 mm diameter stainless steel probe
In the current study, chestnut flour at different levels was incorpo- (P/5) was used to compress a single snack. The peak force indicated
rated into brown rice flour based snack. The objective of this work by the force time curve was taken as the maximum compressive
was to formulate gluten-free fried snacks made from brown rice force/hardness.
flour and chestnut flour and to evaluate their technological, nutri-
tional and sensory properties. 2.5. Colour

2. Materials and methods The colour of snacks was determined by CIE colour scales L⁄, a⁄
and b⁄ using Hunter Lab digital colorimeter (Model D25 M, Hunter
2.1. Materials Associates Laboratory, Reston, USA). Calibration with black and
white standards was performed before colour measurement. The
Paddy variety K-332 was procured from Mountain Research total colour difference (DE) was estimated as:
Centre for Field Crops, Khudwani, Sher-e-Kashmir University of qffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi
2
Agriculture Science and Technology of Kashmir, Jammu and Kash- Total colour difference ðDEÞ ¼ ðDLÞ2 þ ðDaÞ2 þ ðDbÞ
mir. The grains were dried and dehusked in a THU-34A Stake Test-
ing Rice Husker (Stake, Japan) to obtain brown rice. The chestnut where
was bought from the local market of Kashmir, Jammu and Kashmir, DL = (Lsample – Lstandard)
India and dried in hot air oven at 40 °C. Brown rice and chestnut Da = (asample – astandard)
was grounded using Mini Grain Mill (A11B, IKA Inc.) and sifted Da = (bsample – bstandard)
through 300 lm sieve and kept in a refrigerator at about 4 °C for
analysis. 2.6. Expansion ratio

2.2. Preparation of product The diameters of snacks were measured using Vernier calliper
at different positions per sample. Expansion ratio of the snacks
Optimized schedule was taken for the development of product was determined by dividing the average diameter of the sample
which was finalized through initial investigation. Brown rice was by the diameter of the die.
soaked in water for 3 h at room temperature and then dried at
40 °C for 5 h. Flour was obtained by grinding the brown rice using 2.7. Mineral analysis
Mini Grain Mill (A11B, IKA Inc.). It was sifted through the 300 lm
sieve and roasted on pan 100 °C for 10 min. The brown rice and The analysis of minerals was performed using WD-XRF (Wave-
chestnut flour were mixed in the ratio of 100/0, 80/20, 60/40 and length dispersive spectrometer-X ray fluorescence), Bruker AXS,
40/60, respectively. The flour blends was mixed with 2.5% salt, S4-Pioneer Germany by using the method described by Mir et al.
15% hydrogenated vegetable shortening and optimum level of (2016). Two grams of the samples was crushed and mixed with
water. Dough was then shaped in manual extruder with 10 mm 0.5 g boric acid (granulated) with a mortar and pestle. The pre-
circular shaped dies and finally fried in sunflower oil at 150 °C pared sample was then made into a 34 mm diameter pellet with
for five minutes. After cooling for 5 min to room temperature, the help of a 40 ton hydraulic press machine (10 ton pressure,
the snacks were stored in plastic container and kept at room tem- 20 min. pressing time). The pellets were then introduced in the
perature for further investigation. sample slots of WD-XRF and analyzed for the composition of ele-
ments in the samples.
2.3. Pasting properties
2.8. Microstructure
Pasting characteristics of rice-chestnut flour blends were deter-
mined by using Rapid Visco Analyzer (Starch master 2, Newport Microstructure studies were carried out using scanning elec-
Scientific Pty. Ltd, Warriewood, Australia). Powder sample of 3 g tronic microscopy (Hitachi, S-3400N, Tokyo, Japan). The developed
of was weighed in RVA canisters followed by addition of 25 ml of defatted dough and snack were dried in freeze drier and then cut
water. The prepared slurry in the canister was heated to 50 °C into pieces and were mounted on aluminium stubs using double
and stirred at 160 rpm for 10 s to enable the complete dispersion. sided adhesive tape to which the samples were fixed and after-
The slurry was held at 50 °C for 1 min and temperature was raised wards were coated with a thin layer of gold. An acceleration poten-
to 95 °C for 7.5 min. and subsequently held at 95 °C for 5 min. The tial of 15 kV was used during micrography.
slurry was cooled at 50 °C for 7.5 min, and then held at 50 °C for
2 min. Pasting temperature, peak viscosity, hold viscosity, final vis- 2.9. Sensory evaluation
cosity, breakdown viscosity and setback viscosity were
determined. Sensory evaluation of the product was conducted based on 9
point hedonic scale for appearance, colour, flavour, texture and
2.4. Hardness overall acceptability. A semi trained panel of 15 members was
selected to evaluate the sensory properties of snacks. The sensory
The hardness of snacks was measured using a Texture Analyzer, evaluation was performed in laboratory with clean sensory cabi-
TA (TA – HD plus, Stable Micro Systems, Surrey, U.K). A single com- nets containing fresh water. The panellists were instructed to eval-
pression force-versus time program was used to compress along uate the above attributes of the samples and to rate each attribute.
the thickness of sample at a test speed of 0.10 mm/s and return A nine point hedonic scale with 1 (dislike extremely), 5 (neither
to its original position. The original clearance between the probe like nor dislike) and 9 (like extremely) was used.

Please cite this article in press as: Mir, S.A., et al. Technological and nutritional properties of gluten-free snacks based on brown rice and chestnut flour.
Journal of the Saudi Society of Agricultural Sciences (2017), http://dx.doi.org/10.1016/j.jssas.2017.02.002
S.A. Mir et al. / Journal of the Saudi Society of Agricultural Sciences xxx (2017) xxx–xxx 3

2.10. Statistical analysis Hardness value of snacks analysed by texture analyzer was
decreased progressively with the substitution level of chestnut
The data were analyzed statistically using SPSS 18.0 (SPSS Inc., flour (Table 2). The frying process modifies the physicochemical
Chicago, USA,) and the means were separated using the Duncan’s properties of food products which lead to the textural changes.
multiple range test (p < 0.05). All the data are presented as the The chestnut flour substitution decreased the hardness value from
mean with the standard deviation. 58.20 to 42.83 N for 60% blended and control snack, respectively.
The decrease in hardness with chestnut flour incorporation may
be due to high sugar content of the chestnut flour which may influ-
3. Results and discussion ence the starch gelatinization during the frying process and leads
to the texture changes. Sugars are known to delay starch gela-
3.1. Pasting properties of flour blends tinization by reducing the water activity and stabilizing the amor-
phous regions of the starch granule by interacting with starch
The pasting properties of brown rice-chestnut flours blends are linkages (Sumnu et al., 2000), which may be lead to influence the
presented in Table 1. Peak viscosity increased significantly hardness of snack.
(p < 0.05) with progressive increase in chestnut flour level and ran- Expansion ratio of snacks was increased with increase in chest-
ged from 2503 to 3039 cP for flour blends. The increase in peak vis- nut flour level in the blend (Table 2). The high temperature input
cosity by increasing the chestnut flour level indicates the higher during frying caused the water evaporation resulting in volume
viscosity of chestnut flour sample. Chestnut flour having higher expansion of fried products (Mehta and Swinburn, 2001). The high-
peak viscosity indicates a greater structural rigidity in comparison est expansion ratio was shown by 60% chestnut flour blend snack
to rice flour. The result indicated that chestnut flour addition sig- (1.17). The chestnut flour based snacks produced the more
nificantly alter the swelling properties of flour blends. Zaidul expanded products due to the higher starch and lower fibre and
et al. (2007) also observed an increase in the peak viscosity of protein content as compared to control. The higher peak viscosity
wheat flour when mixed with starches from potato, cassava and values were shown by 60% chestnut flour level also confirmed
yam. The structural differences in the amylopectin molecules of the low fibre content of 60% chestnut flour level, which influences
the flour sources may be a potential factor in the increase in peak the expansion ratio of snacks. Maneerote et al. (2009) reported that
viscosity (Bahnassey and Breene, 1994). Similarly breakdown, set- protein reduced the expansion ratio of snacks while studying the
back, and final viscosity were observed to increase significantly deep fried rice crackers.
(p < 0.05) with increase in chestnut flour substitution level to the
flour blend. Yang and Chang (1999) reported that proteins in rice
3.3. Mineral analysis of snacks
flour restrict starch granules swelling and reduce the viscosity.
There was a marked decrease in pasting temperature as the chest-
Mineral analysis done by WD-X-ray fluorescence is presented in
nut flour level increased in the blend from 91.40 to 82 °C. The dif-
Table 3. The 100% brown rice flour based snack was found rich in
ference in pasting temperature is an indication of different
magnesium, manganese, phosphorus and sodium minerals,
gelatinization temperature of flour blends (Yadav et al., 2014).
whereas calcium, chlorine, iron, potassium, sulphur and silicon
The results implies that the increasing level of chestnut flours sub-
content was increased progressively with chestnut flour level in
stitution has a tendency of decreasing the gelatinization
the blend, which indicates that chestnut flour is rich in these min-
temperature.
erals. Nazzaro et al. (2011) reported that chestnut fruit is rich in
essential minerals like calcium, potassium, iron and sodium. The
3.2. Colour, hardness and expansion ratio of snacks minerals present in major quantities in snacks are chlorine
(284.95–307.64 mg/100 g) followed by potassium (60.77–
Colour is one of the important attribute in snacks primarily 94.74 mg/100 g) and phosphorus (34.65–45.56 mg/100 g) in
judged by the consumer. Significant variation (p < 0.05) was snacks. Brown rice has been represents to be rich in essential nutri-
observed in lightness value (L⁄) and ranged from 41.92 to 46.70 ents (Wang et al., 2011), which can be used as raw material for the
for snacks (Table 2). Incorporation of chestnut flour decreased development of functional foods. Although the brown rice flour is
the L⁄, a⁄ and b⁄ value with increasing its substitution level. The rich in functional components, the addition of chestnut flour also
total colour difference (DE) of snacks significantly (p < 0.05) enhances the nutritional value and flavour of the snack. Increasing
increased with chestnut flour substitution level. Progressive supply of such products on the market may be regarded as health
increase in chestnut flour promotes the colour change, which led promoting functional foods, especially for celiac disease patients.
to the increased browning in snacks. This might be probably due
to higher sugar content of chestnut which enhances the brown col- 3.4. Microstructure
our thorough caramelization and Maillard reaction during the fry-
ing operation. The Maillard reaction between reducing sugars and Fig. 1(a) and (c) are the micrographs of 100% rice flour and 60%
amino acids is the primary colour formation reaction during frying chestnut flour substitution dough, respectively. The 100% rice
operation (Baik and Mittal, 2003). Demirkesen et al. (2010) dough consisted of apparently small densely packed starch gran-
reported that increase in chestnut flour in gluten bread resulted ules with interrupted protein matrix. However, the 60% substitu-
in brown colour of breads. tion chestnut flour dough showed larger starch granules and less

Table 1
Effect of different brown rice-chestnut flour blends on pasting properties.

Replacement level (%) Peak viscosity (cP) Hold viscosity (cP) Final viscosity (cP) Breakdown viscosity (cP) Setbak viscosity (cP) Pasting temperature (°C)
0 2503 ± 45.96d 1601 ± 14.12d 3705 ± 53.03d 887 ± 22.12c 2117 ± 30.56b 91.40 ± 0.30a
20 2619 ± 26.16c 1708 ± 1.41c 3820 ± 41.71c 906 ± 26.18c 2131 ± 37.73ab 89.10 ± 0.70b
40 2836 ± 31.11b 1884 ± 16.26b 4009 ± 38.89b 943 ± 21.14b 2143 ± 35.68ab 84.80 ± 0.35c
60 3039 ± 21.21a 2010 ± 5.65a 4196 ± 44.26a 1021 ± 19.23a 2208 ± 67.12a 82.00 ± 0.50d

Values are expressed as mean ± standard deviation. Means in the same column with different letters were significantly different at p < 0.05.

Please cite this article in press as: Mir, S.A., et al. Technological and nutritional properties of gluten-free snacks based on brown rice and chestnut flour.
Journal of the Saudi Society of Agricultural Sciences (2017), http://dx.doi.org/10.1016/j.jssas.2017.02.002
4 S.A. Mir et al. / Journal of the Saudi Society of Agricultural Sciences xxx (2017) xxx–xxx

Table 2
Effect of chestnut flour on color, expansion ratio and texture of rice snacks.

Replacement level (%) L* a* b* DE Expansion ratio Hardness (N)


a a a b
0 46.70 ± 0.45 10.40 ± 0.18 24.58 ± 0.50 – 1.07 ± 0.01 58.20 ± 5.12a
20 45.48 ± 0.61a 9.70 ± 0.23ab 22.45 ± 0.37b 1.06 ± 0.18c 1.07 ± 0.00b 57.23 ± 3.11a
40 42.90 ± 0.56b 9.26 ± 0.31b 20.54 ± 0.51c 4.20 ± 0.10b 1.11 ± 0.02a 47.39 ± 4.20b
60 41.92 ± 0.35b 9.44 ± 0.30b 20.25 ± 0.57c 6.62 ± 0.06a 1.15 ± 0.01a 42.83 ± 2.81b

Values are expressed as mean ± standard deviation. Means in the same column with different letters were significantly different at p < 0.05.

Table 3
Mineral analysis of brown rice-chestnut flour blended snack.

Replacement Calcium Chlorine Iron Potassium Magnesium Manganese Sodium Phosphorus Sulphur
level (mg/100 g) (mg/100 g) (mg/100 g) (mg/100 g) (mg/100 g) (mg/100 g) (mg/100 g) (mg/100 g) (mg/100 g)
0 g/100 g 6.38 ± 0.46c 284.95 ± 3.81c 5.11 ± 0.07c 58.63 ± 0.83c 12.16 ± 0.15a 1.66 ± 0.04a 33.01 ± 0.76a 45.56 ± 0.71a 12.50 ± 0.10d
20/100 g 8.03 ± 0.12a 277.13 ± 3.53d 5.18 ± 0.13c 60.77 ± 0.98c 8.19 ± 0.19b 1.37 ± 0.03b 31.95 ± 1.07a 40.75 ± 1.67b 15.21 ± 0.16c
40/100 g 8.26 ± 0.20a 307.64 ± 4.91a 6.85 ± 0.07b 73.83 ± 0.94b 8.26 ± 0.27c 1.38 ± 0.02b 30.72 ± 0.95a 35.99 ± 1.13c 15.93 ± 0.09b
60 g/100 g 7.10 ± 0.16b 301.13 ± 3.46b 9.46 ± 0.19a 94.74 ± 0.98a 5.01 ± 0.12d 1.07 ± 0.04c 27.53 ± 0.73b 34.65 ± 0.89c 20.31 ± 0.21a

Values are expressed as mean ± standard deviation. Means in the same column with different letters were significantly different at p < 0.05.

Fig. 1. Scanning electronic micrographs (a) 100% rice flour dough; (b) 100% rice flour snack; (c) 60/40 chestnut-rice flour dough; (d) 60/40 chestnut-rice flour snack.

protein matrix in comparison to 100% rice flour dough. The inter- snacks containing 60% chestnut flour having more cells with larger
action of rice and chestnut flour starch and protein leads to the cavities as compared to fried snacks containing 100% rice flour. The
physical change in the developed snack and hence affects its 100% flour rice snack internal structure were more compact and
expansion ratio. The increase percentage of chestnut flour leads relatively more homogeneous as compared to the 60% chestnut
to low protein content in 60% dough and thus increased the expan- flour which may be the reason for the low expansion ratio of
sion ratio of snacks. Fig. 1(b and d) shows the cross section of fried 100% rice flour snack. Torbica et al. (2012) reported that the

Please cite this article in press as: Mir, S.A., et al. Technological and nutritional properties of gluten-free snacks based on brown rice and chestnut flour.
Journal of the Saudi Society of Agricultural Sciences (2017), http://dx.doi.org/10.1016/j.jssas.2017.02.002
S.A. Mir et al. / Journal of the Saudi Society of Agricultural Sciences xxx (2017) xxx–xxx 5

Table 4
Sensory evaluation of snacks form brown rice-chestnut flour blends.

Replacement level (%) Color Appearance Texture Flavour Overall acceptability


ab a a c
0 7.13 ± 0.19 7.08 ± 0.06 7.06 ± 0.07 6.01 ± 0.14 6.97 ± 0.11a
20 7.10 ± 0.14ab 7.16 ± 0.19a 7.00 ± 0.12a 6.31 ± 0.19b 7.04 ± 0.17a
40 7.39 ± 0.07a 7.10 ± 0.12a 6.27 ± 0.18b 7.39 ± 0.26a 7.18 ± 0.12a
60 6.92 ± 0.18b 6.87 ± 0.28a 6.09 ± 0.12b 7.94 ± 0.23a 7.10 ± 0.21a

Values are expressed as mean ± standard deviation. Means in the same column with different letters were significantly different at p < 0.05.

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Please cite this article in press as: Mir, S.A., et al. Technological and nutritional properties of gluten-free snacks based on brown rice and chestnut flour.
Journal of the Saudi Society of Agricultural Sciences (2017), http://dx.doi.org/10.1016/j.jssas.2017.02.002
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Please cite this article in press as: Mir, S.A., et al. Technological and nutritional properties of gluten-free snacks based on brown rice and chestnut flour.
Journal of the Saudi Society of Agricultural Sciences (2017), http://dx.doi.org/10.1016/j.jssas.2017.02.002

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