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Can be decolourized to accept counter Retain cry stal v iolet dye and stain dark
stain (Safranin or Fuchsine); stain red v iolet or purple, they remain coloured
Gram reaction or pink, they don't retain the Gram blue or purple with gram stain when
stain when washed with absolute washed with absolute alcohol and
alcohol and acetone. water.
Lipid and lipoprotein High (due to presence of outer Low (acid-fast bacteria have lipids
content membrane) linked to peptidoglycan)
The cell wall is 7 0 -120 Armstrong thick The cell wall is 1 00 -120 Armstrong
two lay ered.The lipid content is 20 - thick, single lay ered. The Lipid content
Cell wall com position
30% (High), whereas Murein content of the cell wall is low , whereas Murein
is 1 0-20% (Low). content is 7 0-80% (Higher).
Gram -negative Bacteria Gram -positive Bacteria
Attached to each of the muramic acid molecules are a tetrapeptide consisting of both D- and L- amino
acids, the precise composition of which differs between bacteria.
Special Aminoacids found in Peptidoglycan layer:
Diaminopimelic acid: Unique to bacterial cells.
D- alanine: Involved in the cross links between tetrapeptides and in the action of penicillin.
Functions of Peptidoglycan Layer
1. It provides rigid support to bacterial cells and maintains the characteristic shape of the cell.
2. Allows bacterial cell to withstand media of low osmotic pressure, such as water.
Medical Importance of Peptidoglycan Layer
Peptidoglycan is a good target for antibacterial drugs. Eg. Penicillins, cephalosporins etc inhibit
transpeptidase reaction which makes cross-links between the two adjacent tetrapeptides.
Lysozyme enzyme present in human tears, mucus, and saliva cleave peptidoglycan backbone breaking
its glycosyl bonds.
plasmid (ˈplæzmɪd)
—n
a small circle of bacterial DNA that is independent of the main bacterial chro
mosome. Plasmids oftencontain genes for drug resistances and can be trans
mitted between bacteria of the same and differentspecies: used in genetic e
ngineering
Outer layer of cellwall of gram negative bacteria also called endotoxin. It is a characteristics feature of
Gram Negative Bacteria. As in peptidoglycan biosynthesis, LPS molecules are assembled at the plasma
or inner membrane.
Exception: Only one Gram Positive Bacteria, i.e. Listeria monocyotogenes has been found to
contain an authentic Lipopolysaccharide.
Lipopolysaccharide is Pyrogenic (Responsible for fever), and also causes endotoxic shock etc.The LPS is
composed of three distinct units.
A phospholipid called Lipid A, which is responsible for toxic effects
A core polysaccharide of fiver sugars linked through ketodeoxyoctulonate (KDO) to lipid A
An outer polysaccharide consisting of up to 25 repeating units of 3-5 sugars, also called somatic or O
antigen. O antigens are used to identify certain organisms in microbiology laboratory.
Danish scientist Hans Christian Gram devised a method to differentiate two types of bacteria based on the structural
differences in their cell walls. In his test, bacteria that retain the crystal violet dye do so because of a thick layer of
peptidoglycan and are called Gram-positive bacteria. In contrast,Gram-negative bacteria do not retain the violet dye
and are colored red or pink. Compared with Gram-positive bacteria, Gram-negative bacteria are more resistant
against antibodies because of their impenetrable cell wall. These bacteria have a wide variety of applications ranging
from medical treatment to industrial use and Swiss cheese production.