September | October 2010

Feature title: Ecobiol Aqua the effective single strain probiotic

International Aquafeed is published five times a year by Perendale Publishers Ltd of the United Kingdom.
All data is published in good faith, based on information received, and while every care is taken to prevent inaccuracies,
the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis of
information published.
©Copyright 2009 Perendale Publishers Ltd. All rights reserved. No part of this publication may be reproduced in any form
or by any means without prior permission of the copyright owner. Printed by Perendale Publishers Ltd. ISSN: 1464-0058

The International magazine for the aquaculture feed industry

 F: Probiotic F: Probiotic

in the probiotic group, 5% higher then faciens (nor-

Table 2:- Effect of the addition of a B. amyloliquefaciens probiotic on mortality
the 302 units in the control group. The mal content
Treatment Ponds Surface (has) Initial animals Mortality (%)
Rising feed Escalating Opportunistic Environmental
protease activity was 7.47 units in the Low of Shrimp
bacteria
cost fish meal price diseases impact
probiotic group, almost two times the four & fish in oneprices
tonne
units measured in the control group. The of feed) pro- Control 3 24.5 1960000 39.34
differences in the lipase activity were not duce 1.8kg Ecobiol Aqua 3 24.5 1960000 33.50
so high, only 2.5% higher, 40.32 units in the of lactic
probiotic group and 39.1 in the control acid. When

Tired of hearing only bad news ?

Table 3:- Effect of the addition of a B. amyloliquefaciens probiotic on production
group. Ecobiol pro-
From this we deduce that this enzyme duces the Final
Surface Initial Final Body
production improves not only protein, lactic acid in Treatment Ponds Biomass
(ha) animals Weight (g)
Feed is the main cost in most aquaculture starch and fat operations
digestibility but … also the the fish’s gut, (Ton)

Ecobiol Aqua
and the most difficult one to reduce when energy ingredient prices
digestibility, are rising
as these nutrients … it controls
are essential for the energy of the diet pathogenic Control 3 24.5 1960000 19.6 16.90
calculation. bacteria and Ecobiol Aqua 3 24.5 1960000 23.6 18.16
Ecobiol Aqua controls intestinal pH by is used as
producing lactic acid substrate for growth by beneficial bacteria Barstar (produced by the same bacteria but
Performance Preservation Health and Feed and Sensory
Bacteria ferment carbohydrates to (lactobacilli). Several trials have demon- not ejected to the media), acts as a natural
Enhancement & Stabilization Well-Being Food Safety Improvement
obtain energy following different biochemi- strated the beneficial effect that lactic acid antibiotic.

the effective single strain probiotic AQUAGEST®

AQUABITE®
maximizes digestibility and feed utilization efficiency

enhances palatability and appetite

cal pathways; in the case of B. amyloliquefa-
ciens, carbohydrates are normally degraded
following the lactic acid route.
has on fish growth.
Ecobiol Aqua actively balance intestinal
microbiota
Barstar is active at all pHs and is effective
inhibiting the transcription of the genetic
material into protein in all kinds of bacteria,
It has been observed that for every gram Once the spore is transformed into Gram negative (E. coli, Vibrio) and Gram
SANACORE® GM improves growth and productivity by promoting applying nature for a healthy and sustainable future
of sugar present in the media, B. amylolique- the active form of the bacteria, the B. positive (clostridia).
a healthy gut microflora
faciens is able to produce 0.9g of lactic acid. amyloliquefaciens produces Barnase to The inhibition of growth of those bac-
If there are enough carbohydrates in the avoid competition for the loci. Barnase is teria has been demonstrated in different
info.aqua@nutriad.net • www.nutriad.net

P
environment, 1012 CFU of B. amylolique- a ribonuclease that, without its inhibitor, studies evaluating the bactericide capacity
robiotics are viable cultures algal population densities are very high, the spores of the B. amyloliquefaciens
IAF11-PellPress 10.12.2008 10:52 Uhr Seite 1
of microorganisms which, Vibrio numbers are much higher compared can endure pelleting processes of up to
when added to the animal to the normal open water situation. In 90ºC (see Table 1). If the feed processing
feed, can reach and colonise these cases it a defence against over- reaches higher temperatures
10TH PRACTICAL SHORT thanCOURSE:
this, it is Are you sure
the animal’s digestive system, benefi- exposure to Vibrio is necessary (whose Aquafeed Platform
recommended to apply - Aquaculture
the product Feed Extrusion,
post I‘m not missing
Nutrition,
Flat Die Pelleting Presses
cially improving the properties of the pathogenicity may have been increased pelleting (mixed with&fish Feedoil,Formulation
for example).
“Het Pand”, Ghent, University,
a key essential
indigenous flora and interacting with by the abusive use of antibiotic growth The effect of Ecobiol Aqua isGhent, due to Belgium
for
for small,
small, nutrient?
the pathogenic bacteria. promoters). several reasons:November 8 - 9, 2010 medium,
medium,
www.smartshortcourses.com
Ecobiol Aqua improves nutrient digest- and
and high
high
Probiotics in aquaculture can work on Survive in adverse ibility of the diet because
MARKET TRENDSof the produc- capacities
capacities
two completely different situations: on conditions Current and Future Market Trends in Aquaculture
tion of enzymes once inside the animal
one hand, they can control pathogenic Ecobiol Aqua is a probiotic developed The name of the bacteria
FEED INGREDIENTS included in
Ingredient Trends and the Effects on the Extrusion Process; Utilization of Algae in
ONLINE ARCHIVE
bacteria population in animals’ intestines by Norel SA that is Ecobiol Aqua,
Aquafeed and Nutrition; Raw Material Bottlenecks B. amy-in Formulation
(competitive exclusion, bacteriocines), as made up exclusively Table 1: - B. amyloliquefaciens loliquefaciens, was given You can find back issues of
PRACTICAL CONSIDERATIONS IN FEED FORMULATION
well as help the animal digest the feed and of spores of Bacillus counts (CFU/g of feed)
Feed before and Nutrition;after
Formulation Artemia,observing
Enrichments the wayFeed Additives for
and Other International Aquafeed on
and after pelleting (90ºC)Larval Nutrition and Starter Feeds; Shrimp Feed: Formulation and Stability;
Hatcheries;
maintain good health; they are considered amyloliquefaciens. starch was hydrolysed
Rapid Analytical Methods for Ingredient Testing our website, available to view
as a good option to antibiotic growth
promoters.
The spore is a struc-
ture that is adopted by
Before pellet After pellet in the presence
FEED MANUFACTURING TECHNOLOGY
Grinding for Aquatic Feed; Feedbacteria.
PreparationBut
of these
B. amyloliq-
Technology; Feed Coating, Absorption
completely free of charge.
Individual features fromn
VANNAGEN ®
Coating by Vacuum and Micro Encapsulation; Feed Pelletization; Proper Dosification &
On the other hand, the addition of live the bacteria to survive 2.60 x 106 2.25 x 106
Mixing of Macro-Minor uefaciens
Ingredients produces
and Liquids fornotAquafeed, the pages of International
bacteria to the ponds where the animals in adverse conditions. 1.68 x 106 only
1.50 x 106 AQUATIC FEED EXTRUSION amylases: it also Aquafeed can also be found naturally supports…
are farmed may modify the bacterial com- During the produc- Fundamentals of Introduction to produces proteases
Extrusion Technology; andIssues in Extruder
Critical on www.docstoc.com/profile/ … Per formance
1.27 x 106Process Control;
1.16 xAquatic
106 Feed Extrusion: Applications of Twin Screw Extruders; New
position of the water and sediment. tion process in León lipases that help the ani-
Extrusion Technology with Online Control of Product Density and Specific Mechanical perendale … Health
Energy Input; High Fat Extrusion for Aquafeed; Micro Aquatic Feeds; Drying of
In aquaculture, it is easier for the (Spain), the vegetative mal Aquafeed
digesting the feed. … Stress management
opportunistic pathogenic bacteria to bacteria of B. amyloliquefaciens are forced In a study performed with Ecobiol
PLANT DESIGN
colonise the gastrointestinal tract of the into the spore form. This confers the bac-CriticalAqua in and
Parameters shrimp feedfor(presented
Plant Design at the Issues: Noise and
Aquafeed; Environmental www.aquafeed.co.uk/archive.php
Odor Control; Post Processing and Handling of Quality Aquafeed
animals because they are supported by teria a very stable presentation for storage ASAIM SE Aquaculture Conference 2010),

the environment where the animals are
farmed.
For example, Vibrio bacteria are nor-
mally found attached to algae and forms
part of the normal flora of fish and shrimps
www.chemoforma.com
and feed processing, without affecting its it was observed QUALITY that two hours after the
ASSURANCE
capacity to acquire the vegetative form Aquafeed Quality: Lipid Oxidation
administration of feed, and Palatability;
the animals Chemical and Microbiological
that
Aspects; Mycotoxins Management in Aquafeed; Verifying End Product Quality
once the environment is favourable for its were receiving Ecobiol in the feed - at a AMANDUS KAHL GmbH & Co. KG
CONTACTS
development (that is, inside the animals, ratio of 106 CFU/gram of feed - had higher Dieselstrasse 5-9, D-21465 Reinbek / Hamburg
Dr. Ignace Debruyne Phone: +49 40 727 71 0, Fax: +49 40 727 71 100
Dr. Sefa Koseoglu We have your per for mance in mind
where there are nutrients, adequate tem-ID&A Ignace enzymatic activity
Debruyne & Associatesthan the control animals
Filtration and Membrane World LLC
info@amandus-kahl-group.de

as well as zooplankton. perature and moisture). Telephone:

(no +32 (0)51 31 12 74
probiotic). Telephone: +1 979 764 8360 www.akahl.de
Fax: +32 (0)51 31 56 75 Fax: +1 979 694 7031
In aquaculture ponds, where animal and The amylase activity was sefa.koseoglu@membraneworld.com
Different trials have demonstrated thatEmai: aquafeed@scarlet.be 315.4 units Chemoforma Ltd. CH-4302 Augst Switzerland
Tel +41 61 811 33 55 Fax +41 61 811 28 03

18 | InternatIonal AquAFeed | September-october 2010 September-october 2010 | InternatIonal AquAFeed | 19

AquAFeed | September-october 2010 September-october 2010 | InternatIonal AquAFeed | 5
 F: Probiotic Fatten up your bottom line. Buhler high-performance animal and aqua feed production
systems are used by leading companies around the world. These producers know they
so the quality of the water can rely not just on the technology itself, but also on the support that accompanies it. A
quality becomes increas- service combining local presence with global expertise both lowers feed mill operating
ingly important for animal
survival. costs and increases capacity utilization. So the question is not whether you can afford to
Mortality is also
reduced as the probiotic choose Buhler – it’s how a solution from Buhler will feed your profits. To find out more,
improves animal intestinal
visit www.buhlergroup.com.
health and hence, general
health status; it being more
difficult for pathogens
(even virus) to successfully
infect an individual.
Figure 1: Inhibition halos of Vibrio
In a field trial run with
parahaemolyticus CECT 511 around the wells of
Ecobiol Aqua supernate a total of six ponds, 49ha
and 3,920,000 shrimp, the
Bühler AG, Feed & Biomass, CH-9240 Uzwil, Switzerland, T +41 71 955 11 11, F +41 71 955 28 96
mortality was reduced
fu.buz@buhlergroup.com, www.buhlergroup.com
more than 5%.
Ecobiol Aqua improves
feed conversion ratio and
growth (animal size)
Due to all the afore-
mentioned benefits:
enzyme production, lactic
acid excretion to the
lumen, intestinal micro-
biota balance, better ani-
mal health, etc, the animals
are able to perform better
Figure 2: Performance parameters in Litopaneus from a zootechnical point
vannamei juveniles fed a control diet, or a diet of view.
including B. amyloliquefaciens probiotic
Ecobiol Aqua is effec-
tive in both young and
of the supernate of B. amyloliquefaciens. adult, productive animals.
The supernate of the fermentation of B. The effect on young animals has been
amyloliquefaciens is placed in six mm wells, demonstrated in different trials (see Figure
and the halo around that well where the dif- 2). It helps the earlier uptake of feed and
ferent shed bacteria are not able to grow is avoids health problems.
measured: the bigger the halo, the stronger With adult animals, when the density in
the inhibition. the ponds increases and the water quality
The combination of the Barnase produc- worsens, the effect of the probiotic is even
tion, that inhibits the growth of pathogenic more effective (see Table 3).
bacteria populations, with the lactic acid
production, that improves the lactic flora Conclusion
population, ends up in a well-balanced, The usual probiotic products used in
steady and healthy intestinal microbiota in aquaculture are composed of different "Ecobiol Aqua
the fish, which can be observed as better strains of different bacteria that do not have
health and more resistance to illnesses in well defined effects. Ecobiol Aqua is a single
is a single
the animals. strain probiotic effective at many different strain probiotic
levels that ensures safe and economically
Ecobiol Aqua reduces profitable production. effective at many
mortality in the ponds
As mentioned before, the use of the
different levels
probiotic Ecobiol Aqua improves nutrient More inforMation: that ensures safe
digestibility. This fact has a direct effect Juan José Mallo, Ingeniero Agrónomo
on the water quality, there is less organic Technical Director and economically
matter in the water, and whatever organic Norel S.A.
matter is in there, can be degraded by P.I. Can Coll, c/ Indstria, 1 profitable
(08185) Lli de Vall, Barcelona Spain
the bacteria included in the probiotic. In Website: www.norelynature.com production"
aquaculture the animal density is much The solution behind the solution.
higher than in natural open water situations

20 | InternatIonal AquAFeed | September-october 2010

This digital re-print is part of the September | October 2010 edition of International Aquafeed magazine.
Content from the magazine is available to view free-of-charge, both as a full online magazine on our
website, and as an archive of individual features on the docstoc website.
LINKS
Please click here to view our other publications on www.docstoc.com.

VO L U M E 1 3 I S S U E 5 2 0 1 0
• See the full issue
Influence of natural and
artificial binders
- in feeds for Litopenaeus vannamei
• Visit the International Aquafeed website
on digestibility & growth

Ecobiol Aqua
- the effective single strain probiotic
• Contact the International Aquafeed Team
A general overview
aquaculture in the EU

Abalone feed development

in South Africa • Subscribe to International Aquafeed
THE INTERNATIONAL MAGAZINE FOR THE AQUACULTURE FEED INDUSTRY
Member of the World Aquaculture Society, European Aquaculture Society, American Feed Industry Association
and the International Aquafeed Association

IAF10-05.indd 1 07/09/2010 14:54

To purchase a paper copy of the magazine, or to subscribe to the paper edition please contact
our Circulation and Subscriptions Manager on the link above.

INFORMATION FOR ADVERTISERS - CLICK HERE

www.aquafeed.co.uk