Part A – Proposal

Attach risk assessment (after the report)

Part- B
Aim – Different concentration of hydroxide peroxide on the catalyse activity.

Introduction-
Enzymes, what are they?
What’s their function? How do they do that?
Focus on concentration
How can enzymes be affected?
Which concertation works the best? Research 0.1, 0.5 or 1? Why?

Hypothesis-
If independent variable….increases/decreases then the reaction time will increase/decrease.
Refer to Introduction for the choices made.

Variables-
Independent (What are you changing each trial) Hydrogen peroxide concentration
Dependent (What is influenced by the independent variable that you need to measure)
Control (List all the variables held constant)

Constant Why was it kept constant? How was it controlled?

E.g Sources of vegetable may have been stored for longer The same batch of potatoes were
Vegetables and already suffered water loss. used.

Materials

Method

Safety Assessment
Identify specific hazards associated with the practical
Apron,
Potential risk hazard level of risk
Peroxide
Glass – mention what did you do to be safe.
Wore apron so that hydrogen peroxide cannot be dropped to the clothes as it will leave the
stains.

Results
Graph D variable/ Independent variable Mention R^2. Use scatter graph, don’t forget the
units!! IV = x and Dependent variable on y-axis.
Mention bubbles formation (which is oxygen)
Table
Variable Trial 1 Trial 2 Trial 3 Average Standard deviation
0.1%
0.5%
1.0%
Distance of hydrogen peroxide in the test tube

Discussion (Results + Error + Improvements)

Results
- Pattern  linear relationship
- Did the result support the hypothesis?
- Outliers (if any)  Precision (standard deviation) Results are precise if the numbers
are close to the average.
- If the average is 6 and stdv is 4 then the precision is low cos stdv is high. Average = 6,
stdv = 0.1 then precision high stdv low.
-
Errors (Random + systematic)
Importance of replication. Why is it important to repeat the prac? To minimize the random
erros. The more trials the closer to average. there must be a range of errors that would have
affected the results.
Random errors (precision, reliability)
- parallax error  random errors can be removed by repetition  yeast could be less
than 7g- the packet had 7g yeast however all the 7g might not have been consumed.
- Test tubes were rinsed through distilled water however; it might not have removed
all of the hydrogen peroxide.
- 20 ml of hydrogen peroxide was used could be less or more than that.
- Yeast dissolved in 100 ml of water could be less or more.
- Measuring time – stopped too quickly, late.
- Filtered paper kept on paper towel could have removed more of yeast solution
instead of removing the excess.
Systematic errors (accuracy)
- Concentration of solution could have changed because it was used on the next day.
Therefore, could alter the solution
- Faulty equipment
- Hydrogen peroxide might not be the same as mentioned. As 0.1% could be slightly
0.11% etc.
- Methodology, filtered paper was kept in yeast for 3 seconds which could alter the
results it was changed to 5 seconds.

Improvements
Link it back to random errors.

Conclusion
Yes the results supported the hypothesis however there were some errors as mentioned in
the discussion which can be improved by the improvements suggested in the discussions.
According to the results it is suggested that the more the hydrogen’s concentration the faster
the reaction will take place.

State the results again.

Did it support your hypothesis?
Why these results happened, i.e. what is the biology behind it?
Random errors (Due to measurement error, can be minimized by more trials)
Systematic errors (can only be detached by repeating an experiment more than once or
comparing the data to the true value… we don’t have time to repeat)
If it says 7 g and it is 6 then it is systematic
Was it on flat surface?
Thermometer, was it accurate?
Yeast can be old, solution can be old
How can it affect the results? Might not be accurate, if yeast is old the filtered paper take
longer to rise up. Amount could affect, if the solution is less in one of them the filter paper
will rise up quick which will alter the time - Average and standard deviation - graph.
Suggest improvements. Link it back to errors. Why that would assure to get better results?

Conclusion
Results supports the hypothesis. Therefore, it is proven that the more concertation of
hydrogen peroxide it is the faster the reaction takes place.

Reference list
Peer review
Appendix
Graphs and values of trial 1,2 and 3.