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Essential Medium
M
inimum Essential Medium is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet
the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM
includes higher concentration of amino acids so as to closely approximate the protein composition of cultured
mammalian cells. MEM can be used either with Earle's salts and Hank's salts and can also be additionally supplemented with
non-essential amino acids (NEAA). The modification can be further modified by eliminating calcium to facilitate growth of cells
in suspension cultures.
HiMedia Competitor 1
0.6
Cell count in (millions/ml)
0.5
0.4
0.3
0.2
0.1
0
MDCK CHO Vero Hep-2 BHK-21 Sp2/0
Cell lines
Fig 1.1: Comparative Performance of HiMedia and Competitior MEM in different cell lines.
HiMedia MEM supplemented with 10% FBS (Fetal Bovine Serum) was compared to competitor MEM for growth promotion test for various cell
lines. Seeding densities varied as per the cell line. Cells were seeded in triplicate in a 24 well plate and incubated at 37°C with 5% CO2 and
95% air over a 4 day passage cycle. Samples were taken daily and evaluated for cell density and morphology. Data depicted in the graph
represents the average count obtained on day 4 of the third passage.
Applications
-Cultivation of wide variety of mammalian cells.
-Recommended to be used with 5-10% FBS.
0.3
HiMedia Competitor 1
0.2
0.1
0
0 1 2 3
No, of Days
CHO cells were seeded at 0.012 X 106cells/ml in triplicate in a 24 well plate plate and incubated at 37°C with 5% CO2 and 95% air.
Samples were taken every 24 hours and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found
comparable to the competitor media.
0.6
HiMedia Competitor 1
0.5
Vero cells (millions/ml)
0.4
0.3
0.2
0.1
0
0 2 3 4 5 6
No. of Days
6
Vero cells were seeded at 0.1 X 10 cells/ml in triplicate in a 24 well plate and incubated at 37°C with 5% CO2 and 95% air. Samples were
taken daily after two days and evaluated for cell density and morphology. Cell growth and morphology of HiMedia was found comparable
to the competitor media.
0.25 HiMedia Competitor 1
0.15
0.1
0.05
0
1 2 3 4 5 6 7 8 9 10
No. of Days
Fig 1.4 Serial subculture of BHK-21 cells
BHK-21 cells were seeded at 0.04 X 106 cells/ml and incubated at 37°C with 5% CO2 and 95% air. Cells were passaged every 24 hours and
evaluated for cell density and morphology. Cell growth and morphology of Himedia media was found comparable to the competitor media.
Ordering Information
Powder Media
Media Specifications Codes
Minimum Essential Medium Eagle (MEM) w/ Earle's salts and L-Glutamine AT020
w/o Sodium bicarbonate and NEAA
Minimum Essential Medium Eagle (MEM) w/ Earle's salts, NEAA and L-Glutamine AT047
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts and NEAA AT047A
w/o L-Glutamine and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts, NEAA and L-Glutamine AT046
w/o Phenol red and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts and NEAA AT046A
w/o L-Glutamine, Phenol red and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts, L-Glutamine and 25mM HEPES buffer AT074
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts, NEAA, L-Glutamine and 25mM HEPES buffer AT153
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts, NEAA, Sodium pyruvate and L-Glutamine AT154
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts and L-Glutamine AT159
w/o Sodium bicarbonate, NEAA and Phenol red
Minimum Essential Medium Eagle (MEM) w/ Earle’s Salts,L-Glutamine, NEAA, Sodium Pyruvate and 15mM HEPES buffer AT182
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Hank's salts and L-Glutamine AT056
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Hanks's salts AT056A
w/o L-Glutamine and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Hank's salts, L-Glutamine and NEAA AT048
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Hank's salts, L-Glutamine and 25mM HEPES buffer AT075
w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts and NEAA AT017A
(Modified for Autoclaving) w/o L-Glutamine and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Earle's salts AT077A
(Modified for Autoclaving) w/o L-Glutamine,NEAA and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Hank's salts and NEAA AT045A
(Modified for Autoclaving) w/o L-Glutamine and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ L-Glutamine AT079
Joklik Modification w/o Calcium chloride and Sodium bicarbonate
(For Suspension Culture)
Minimum Essential Medium Eagle (MEM) w/ L-Glutamine, Ribonucleosides and Deoxyribonucleosides AT080
(Alpha Modification) w/o Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ Ribonucloesides and Deoxyribonucloesides AT080A
(Alpha Modification) w/o L-Glutamine and Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/o L-Glutamine, Ribonucloesides, Deoxyribonucloesides and AT081A
(Alpha Modification) Sodium bicarbonate
Minimum Essential Medium Eagle (MEM) w/ L-Glutamine AT081
(Alpha Modification) w/o Ribonucleosides, Deoxyribonucleosides and Sodium bicarbonate
Ordering Information
Liquid Media
LoSeraTM Minimum Essential Medium Eagle w/ Earle's salts, NEAA and Sodium bicarbonate RSL008
(MEM) w/o L-Glutamine
LoSeraTM Minimum Essential Medium (MEM) w/ Earle's salts, L-Alanyl-L Glutamine, NEAA and sodium bicarbonate RSL008G
v As per ATCC composition • Powder media available in 1L, 5L & 20L packing. • Liquid media available in 5X100ML, 2X500ML, 6X500ML and 18X500ML
• Bulk packing available on request. • Customised Media available on request.