Acct # 3680

Sage - Centers for Veterinary Report No.

Specialty and Emergency Care 18012108
907 Dell Avenue
Campbell, CA 95008

Phone: (408) 343-7243

Name Jasper US10460-DR46983 Sp, Breed Fel, Bengal Collected 1/22/18

Owner Grant, Sylvaine & Adam Sex M Received 1/23/18
Doctor Garcia DOB/Age 06/15/2017 Reported 1/31/18
Copy sent to: Dr. Garcia (e-mail)

HISTORY
Fever with high globulins. Suspected FIP. Scant free abdominal fluid unable to sample. Enlarged spleen,
liver, and an abnormally enlarged lymph node.

SPECIMEN
1. Liver. A, B.
2. Spleen. C - E.
3. Lymph node. F - I.

MICROSCOPIC DESCRIPTION
1. Liver: Numerous cords and clusters of hepatocytes are present in a pale lavender mildly bloody
background. A mild component of diffuse or discrete hepatocellular vacuolation is present in some cells.
Throughout the sample most remaining cells are dispersed individually representing a mixture of leukocytes.
Neutrophils appear nondegenerate and appear mildly increased from those commonly expected to be from
this amount of blood. Most of the remaining cells are small mature lymphocytes seen along with occasional
eosinophils. Few basophilic reactive type lymphocytes are seen as well and there are scattered
microvacuolated macrophages. Macrophages sometimes have amorphous pink material within the
cytoplasm.

2. Spleen: Smears have a bloody background with a stroma composed of mononuclear to spindle cells,
sometimes with associated strands of endothelium. Lymphocytes are most numerous and are sometimes
present in monolayered sheets appearing as a mixture. Small mature lymphocytes are most numerous seen
along with expanded numbers of large deeply basophilic lymphocytes and few mature plasma cells. Few
intermediate granular lymphocytes are seen as well. Histiocytes and macrophages are seen individually and
also sometimes in small groups. Most of the remaining cells are slightly toxic nondegenerate neutrophils.
Scattered eosinophils are seen and there are few polygonal cells with morphology most suggestive for
cosampled mesothelium.

3. Lymph node: The pink bloody background entraps low to moderate numbers of nucleated cells.
Lymphocytes compose a heterogeneous mixture. Small mature lymphocytes are seen along with
intermediate and large, sometimes deeply basophilic, lymphocytes. Neutrophils appear slightly toxic and are
increased from those expected to be from the blood. Macrophages are seen individually and in moderately
sized aggregates in some slides. Macrophages are microvacuolated, erythrophagic/ cytophagic and
sometimes have granular pink amorphous material within the cytoplasm. Mast cells are rare.

DIAGNOSIS
1. Liver: Few mixed inflammatory cells with mild mixed hepatocellular vacuolation.
2. Spleen: Moderate lymphoid reactivity.

Page 1 of 3 Date Printed: 1/31/18 11:28:52AM

 Acct # 3680
Sage - Centers for Veterinary Report No.
Specialty and Emergency Care 18012108
907 Dell Avenue
Campbell, CA 95008

Phone: (408) 343-7243

Name Jasper US10460-DR46983 Sp, Breed Fel, Bengal Collected 1/22/18

Owner Grant, Sylvaine & Adam Sex M Received 1/23/18
Doctor Garcia DOB/Age 06/15/2017 Reported 1/31/18
Copy sent to: Dr. Garcia (e-mail)

3. Lymph node: Mild to moderate lymphoid reactivity with moderate neutrophilic to pyogranulomatous
inflammation.

COMMENT
An inflammatory response is most notable in the lymph node that also has the appearance typical for
reactivity. Inflammatory cells are a predominance of neutrophils and macrophages. Etiologies for this type
of response include occult infection (including FIP, protozoal -toxoplasma, etc), response to regional
inflammatory disease (enteritis, vasculitis, pancreatitis, etc), or other similar type of insult. No evidence of
infectious agents is seen despite searching. No features are present to support neoplasia in the node.
It may be possible to further test for FIP by using Corona virus immunocytochemical stain (recommended
on the node). One of the slides submitted for this case could be de-stained and re-stained using this
technique. If you would like to pursue this possibility please call and request FIP. Results are typically
available in 5-7 working days. A positive result strongly supports a diagnosis of FIP. A negative result does
not completely rule out this diagnosis because cells containing the virus may be located in small foci
throughout the organ.

Changes in the liver and spleen are less remarkable. Organisms and features of neoplasia are not apparent.
Hepatocytes are mildly vacuolated, but this is a non-specific change that can be seen with a variety of
acquired conditions. Few inflammatory cells are present here but a specific etiology is not found.
Cells found in this splenic aspirate arecells typically found in aspirates of this tissue. There are not
increased or abnormal spindle cells, excessive immature round cells or unusual hematopoietic precursors.
Enlargement in the spleen may be due to areas of lymphoid hyperplasia, increased blood
pooling/congestion, changes in trabecular structures or similar type processes.

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ADDENDUM 1/31/18.

Immunocytochemical staining for FIP.

RESULTS- Few macrophages demonstrate some faint intracellular staining which supports that this is
positivity for Corona virus inclusions. This appearance supports presumptive inflammation in the node due
to FIP.

J. Hodges

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Page 2 of 3 Date Printed: 1/31/18 11:28:52AM

 Acct # 3680
Sage - Centers for Veterinary Report No.
Specialty and Emergency Care 18012108
907 Dell Avenue
Campbell, CA 95008

Phone: (408) 343-7243

Name Jasper US10460-DR46983 Sp, Breed Fel, Bengal Collected 1/22/18

Owner Grant, Sylvaine & Adam Sex M Received 1/23/18
Doctor Garcia DOB/Age 06/15/2017 Reported 1/31/18
Copy sent to: Dr. Garcia (e-mail)

PATHOLOGIST
Joanne Hodges, DVM
Diplomate, American College of
Veterinary Pathologists

Veterinarians: For consultation on this report, please call (877) 753-4285.

Full Cytology - 3 Sites

Page 3 of 3 Date Printed: 1/31/18 11:28:52AM