No.

Klasifikasi BAM ISO

1 Method Chapter 3 4833-1: 2013
2 Media Plate Count Agar Plate Count Agar

3 Suhu Inkubasi (⁰C) 35 ± 1 30 ± 1 °C

Susu 32 ± 2
Waktu Inkubasi
4 (Jam) 48 ± 2 72 ± 3
No. Klasifikasi BAM ISO
1 Method Chapter 4 4831 : 2006
2 Media Brilliant green lactose bile (BGLB) broth, 2% Lauryl sulfate tryptose broth
Lauryl tryptose (LST) broth Brilliant green lactose bile broth
EC broth
Levine's eosin-methylene blue (L-EMB) agar
Tryptone (tryptophane) broth
MR-VP broth
Koser's citrate broth
Plate count agar (PCA)
Butterfield's phosphate-buffered dilution water
Kovacs' reagent
Voges-Proskauer (VP) reagents
Gram stain reagents
Methyl red indicator
Violet red bile agar (VRBA)
VRBA-MUG agar
EC-MUG medium
Lauryl tryptose MUG (LST-MUG) broth
Peptone diluent, 0.1%

3 Suhu Inkubasi (⁰C) 35 ± 1 30 or 37

Waktu Inkubasi
4 (Jam) 48 ± 2 24 or 48
24-48 h (solid medium method)
fate tryptose broth
een lactose bile broth
No. Klasifikasi BAM
1 Method Chapter 4
2 Media Brilliant green lactose bile (BGLB) broth, 2%
Lauryl tryptose (LST) broth
EC broth
Levine's eosin-methylene blue (L-EMB) agar
Tryptone (tryptophane) broth
MR-VP broth
Koser's citrate broth
Plate count agar (PCA)
Butterfield's phosphate-buffered dilution water
Kovacs' reagent
Voges-Proskauer (VP) reagents
Gram stain reagents
Methyl red indicator
Violet red bile agar (VRBA)
VRBA-MUG agar
EC-MUG medium
Lauryl tryptose MUG (LST-MUG) broth
Peptone diluent, 0.1%

3 Suhu Inkubasi (⁰C) 35 ± 1

Waktu Inkubasi
4 (Jam) 24 ± 2
 ISO ISO
16654 : 2001 16649-2:2001
Modified tryptone soya broth with novobiocin (mTSB + N) Tryptone-bile-glucuronic medium (TBX)
Cefixime tellurite sorbitol MacConkey agar (CT-SMAC)
Nutrient agar
Tryptone/tryptophan medium
Kovac’s indole reagent
Normal saline solution

37 °C ± 1 °C. 44 °C ± 1 °C
41,5 °C 1 °C

18-24 18-24 h
onic medium (TBX)
No. Klasifikasi BAM ISO
1 Method 21527-1:2008
2 Media Dichloran rose bengal chloramphenicol (DRBC) agar peptone water broth
Dichloran 18% glycerol (DG18) agar Dichloran-rose bengal chloram
Plate count agar (PCA)
100 mg chloramphenicol/liter
Malt agar (MA)
Malt extract agar (Yeasts and Molds)
Potato dextrose agar (PDA)

3 Suhu Inkubasi (⁰C) 25 °C 25 °C ± 1 °C

Waktu Inkubasi
4 (Jam) 120 h 120 h
21527-1:2008
peptone water broth
Dichloran-rose bengal chloramphenicol agar (DRBC)

25 °C ± 1 °C
No. Klasifikasi BAM ISO
1 Method 21528-1:2004
2 Media Buffered brilliant green bile glucose broth
Violet red bile glucose (VRBG) agar
Nutrient agar
Glucose agar
Oksidase reagent

3 Suhu Inkubasi (⁰C) at 37 °C

Waktu Inkubasi
4 (Jam) 24 h ± 2 h.
No. Klasifikasi BAM ISO
1 Method Chapter 5 6579:2002
2 Media Lactose broth Buffered peptone water (BPW)
Nonfat dry milk (reconstituted) Modified semi-solid Rappaport-Vassiliadis (MSRV) agar
Selenite cystine (SC) broth Novobiocin solution
Tetrathionate (TT) broth xylose lysine deoxycholate (XLD) agar;
Rappaport-Vassiliadis (RV) medium
Xylose lysine desoxycholate (XLD) agar
Hektoen enteric (HE) agar
Bismuth sulfite (BS) agar
Triple sugar iron agar (TSI)
Tryptone (tryptophane) broth
Trypticase (tryptic) soy broth
Trypticase soy broth with ferrous sulfate
Lauryl tryptose (LST) broth
Trypticase soy-tryptose broth
MR-VP broth
Simmons citrate agar
Urea broth
Urea broth (rapid)
Malonate broth
Lysine iron agar (LIA) (Edwards and Fife)
Lysine decarboxylase broth
Motility test medium (semisolid)
Potassium cyanide (KCN) broth
Phenol red carbohydrate broth
Purple carbohydrate broth
MacConkey agar
Nutrient broth
Brain heart infusion (BHI) broth
Papain solution, 5%
Cellulase solution, 1%
Tryptose blood agar base
Universal preenrichment broth
Potassium sulfite powder, anhydrous
Chlorine solution, 200 ppm, containing 0.1% sodium dodecyl sulfate
Ethanol, 70%
Kovacs' reagent
Voges-Proskauer (VP) test reagents
Creatine phosphate crystals
Potassium hydroxide solution, 40%
1 N Sodium hydroxide solution
1 N Hydrochloric acid
Brilliant green dye solution, 1%
Bromcresol purple dye solution, 0.2%
Methyl red indicator
Sterile distilled water
Tergitol Anionic 7
Triton X-100
Physiological saline solution, 0.85% (sterile)
Formalinized physiological saline solution
Salmonella polyvalent somatic (O) antiserum
Salmonella polyvalent flagellar (H) antiserum
Salmonella somatic group (O) antisera: A, B, C1, C2, C3, D1, D2, E1, E2, E3, E4, F, G, H, I, Vi, and other groups, as appropriate
SalmonellaSpicer-Edwards flagellar (H) antisera
35 ± 2 °C
3 Suhu Inkubasi (⁰C) 41,5 °C ± 1 °C
Waktu Inkubasi 24 ± 2 h
4 (Jam) 24 h ± 3 h.
No. Klasifikasi BAM ISO
1 Method Chapter 14 7932:2004
2 Media Mannitol-egg yolk-polymyxin (MYP) agar plates Base medium
Egg yolk emulsion, 50% polumixin B solution
Trypticase soy-polymyxin broth (0.1%) egg yolk emulsion
trypticase soy-polymyxin broth (0.15%) complete medium (MYP) agar
Phenol red glucose broth Sheep blood agar
Tyrosine agar Defibrinated sheep medium
Lysozyme broth
Voges-Proskauer medium
Nitrate broth
Nutrient agar for B. cereus
Motility medium (B. cereus)
Trypticase soy-sheep blood agar
Nitrite detection reagents
Butterfield's phosphate-buffered dilution water
Voges-Proskauer test reagents
Creatine crystals
Gram stain reagents
Basic fuchsin staining solution
Methanol

3 Suhu Inkubasi (⁰C) 30 ± 2°C 30 °C

Waktu Inkubasi
4 (Jam) MPN 48h ± 2 (tube), 24-48h (plate) 18 h to 24 h
Plate count 24h
medium (MYP) agar

ed sheep medium
No. Klasifikasi BAM ISO
1 Method Chapter 12 6888-1:1999
2 Media Baird-Parker medium Baird-Parker agar medium
Trypticase (tryptic) soy agar (TSA) Brain-heart infusion broth
Brain heart infusion (BHI) broth Rabbit plasma
Coagulase plasma (rabbit) with EDTA Egg yolk emulsion
Toluidine blue-DNA agar
Lysostaphin
Tryptone yeast extract agar
Paraffin oil, sterile
0.02 M phosphate-saline buffer containing 1% NaCl
Catalase test

3 Suhu Inkubasi (⁰C) 35 35 °C ± 1 °C or 37 °C ± 1 °C

Waktu Inkubasi
4 (Jam) 48 ± 2 24 h ± 2 h
-Parker agar medium
-heart infusion broth

olk emulsion

± 1 °C or 37 °C ± 1 °C
No. Klasifikasi BAM
1 Method Chapter 10
2 Media Acetic acid, 5 N
Acriflavin HCl
Agar (Difco)
N-(1-naphthyl) ethylene diamine
α-naphthol reagent
Blood agar base No. 2 (Unipath)
Cycloheximide
Sheep blood, defibrinated; sheep blood agar
Fluorescent antibody (FA) buffer (Difco)
Ethanol, absolute
Glycine anhydride
Gram stain kit
Hydrogen peroxide solution, 3% for catalase test
KOH, 40% solution
Listeria-typing sera set
Lithium chloride-phenylethanol-moxalactam (LPM) agar
Nalidixic acid (sodium salt)
Nitrate broth and nitrite detection reagents
Nutrient broth
Physiological saline solution, 0.85%
SIM medium (Becton-Dickinson Microbiology Systems:BDMS)
motility test medium
Sulfanilic acid reagent
Purple carbohydrate fermentation broth base
Trypticase soy agar with 0.6% yeast extract (TSAYE)
Trypticase soy broth with 0.6% yeast extract (TSBYE)
Tryptose broth and agar
Oxford medium (OXA)
Buffered enrichment broth
PALCAM agar
Carrageenan

3 Suhu Inkubasi (⁰C) 30 °C

Waktu Inkubasi
4 (Jam) 48h
 ISO
11290-2:1998
Buffered peptone water
Base medium for half-Fraser broth with ammonium iron(III) citrate
PALCAM agar
Tryptone soya yeast extract agar (TSYEA)
Sheep blood agar
Carbohydrate utilization broth (rhamnose and xylose)
Carbohydrate solutions
Motility agar
CAMP (Christie, Atkins, Munch-Paterson) medium and test strains
Hydrogen peroxide solution
Phosphate-buffered saline (PBS)
Sheep blood red cell suspension

35 °C or 37 °C

24 h and 48 h.
No. Klasifikasi BAM ISO
1 Method Chapter 16 7937:2004
2 Media Tryptose-sulfite-cycloserine (TSC) agar Fluid thioglycollate medium
Egg yolk emulsion, 50% Lactose Sulfite Medium (LS)
Chopped liver broth Nitrate motility medium
cooked meat medium (modified) Nitrite detection reagent
Thioglycollate medium (fluid) Sulfite-cycloserine agar
Iron milk medium (modified) Zink dust
Lactose-gelatin medium Lactose gelatin medium
Sporulation broth
Motility-nitrate medium, buffered
Spray's fermentation medium
AE sporulation medium, modified
Duncan-Strong sporulation medium, modified
Peptone diluent
Nitrite detection reagents
Glycerin-salt solution (buffered)
Gram stain reagents
Fermentation test papers.
0.2% aqueous bromthymol blue solution
Bromthymol blue, 0.04% aqueous solution

3 Suhu Inkubasi (⁰C) 35 37

Waktu Inkubasi
4 (Jam) 20 - 24h 24 h ± 2 h
glycollate medium
ulfite Medium (LS)
otility medium
ection reagent
closerine agar

elatin medium
No. Klasifikasi
1 Method
2 Media
3 Suhu Inkubasi (⁰C)

Waktu Inkubasi
4 (Jam)
Chapter 9
AKI broth
Alkaline peptone salt (APS) broth
Alkaline peptone water (APW)
Arginine glucose slant (AGS)
Bicarbonate buffer, 0.1 M, pH 9.6
Blood agar
1% Bovine serum albumin (BSA) in ELISA buffer and in PBS
Brain heart infusion (BHI) agar
Bromcresol purple broth supplemented individually with sucrose, lactose, cellobiose, arabinose, D-mannitol, or D
Cary-Blair transport medium
Casamino acids-yeast extract (CYE) broth
Chicken red blood cells, 2.5%, in physiological saline
Cholera enterotoxin
Christensen's urea agar
Citric acid, 0.05 M, pH 4.0
Coating solution for V. vulnificus EIA
Decarboxylase basal medium , unsupplemented, and supplemented individually with arginine, lysine, or ornithine
ELISA buffer for cholera toxin assay
El Tor phage V (ATCC, Rockville, MD)
Enzyme immunoassay (EIA) wash solution for V. Vulnificus
Fetal calf serum
Gelatin agar and gelatin salt agar
Goat antiserum to cholera toxin
Gram stain dyes
Ham's F-10 medium with glutamine , commercial preparation preferred
Heart infusion broth and agar
Hugh-Leifson glucose broth
1 N hydrochloric acid (HCl)
Kligler iron agar (KIA)
Kovacs' reagent
Long-term preservation medium
Mannitol-maltose agar
Methyl red indicator
Mineral (paraffin) oil, sterile
Modified cellobiose-polymyxin B-colistin (mCPC) agar
Monoclonal antibody to V. Vulnificus
Motility test medium, semisolid
MR-VP broth
Mueller-Hinton agar
Mukerjee phage IV
O/129 (2,4-diamino-6,7-diisopropyl pteridine) disks, 10 and 150 μg
OF medium, semisolid , supplemented individually with glucose, sucrose, lactose, cellobiose, arabinose, D-mann
ONPG test reagents or commercially available disks
Oxidase test reagent
Penicillin-streptomycin (Pen-strep) solution
Peroxidase-conjugated goat immunoglobulin G (anti-mouse; anti-rabbit)
Peroxidase substrate solution (4-chloro-1-naphthol) for membrane ELISA
Peroxidase substrate solution (ABTS)
Phosphate-buffered saline (PBS), pH 7.4
Physiological saline solution, 0.85%
Polymyxin B antibiotic disks, 50 units or commercially prepared
Poly, Inaba, and Ogawa antisera for V. Cholerae
Rabbit antiserum to cholera toxin
Reagents for gene-probe testing,
Reference strains for V. cholerae classical and El Tor biotypes, serogroup O1; cytotoxin-producing strains of V. c
parahaemolyticus; cytotoxin-hemolysin-positive strains of V. vulnificus, and V. Mimicus
Sodium chloride dilution water, 2% and 3%
1 N sodium hydroxide (NaOH) solution
Sheep red blood cells, 5%, in physiological saline
Thiosulfate-citrate-bile salts-sucrose (TCBS) agar
Triple sugar iron (TSI) agar
Tris-buffered saline, pH 7.5 (TBS) ; with 1% or 3% gelatin ; with 0.05% Tween 20 (TBS-Tween
Triton X-100
Trypsin-EDTA solution, 1X
Trypticase (tryptic) soy agar (TSA) and broth (TSB)
Tryptic soy broth (TSB) without dextrose, supplemented with 1% NaCl and 24% glycerol (total 1.5% NaCl), and w
1% Tryptone, 1% NaCl (T1N1) agar (M163) and T1N2 agar
Tryptone broth (1%), unsupplemented (0% NaCl), or supplemented with 1, 3, 6, 8, or 10% NaCl
Voges-Proskauer test reagents
Wagatsuma agar
Y-1 adrenal cell growth medium
Y-l mouse adrenal cells or Chinese hamster ovary (CHO) cells (ATCC)

35-37

18-24h
 ISO
21872-1:2007
Alkaline saline peptone water (ASPW)
Saline nutrient agar (SNA)
Saline triple sugar iron (TSI) agar
Saline medium for detection of ornithine decarboxylase (ODC)
Saline medium for detection of lysine decarboxylase (LDC)
Saline medium for detection of arginine dihydrolase (ADH)
Reagent for detection of β-galactosidase
Saline medium for detection of indole
Saline peptone waters
Sodium chloride solution
37 °C for deep frozen products
41,5 for fresh product

6 h ± 1 h for deep frozen products

6 h ± 1 h for fresh products
No. Klasifikasi
1 Method
2 Media

Biochemicals and reagents

3 Suhu Inkubasi (⁰C)

4 Waktu Inkubasi (Jam)

 BAM ISO
Chapter 7
Campylobacter Enrichment Broth
lysed horse blood
antibiotic supplement
Campylobacter isolation agars
Abeyta-Hunt-Bark (AHB) agar
Modified Campy blood-free agar (mCCDA)
Abeyta-Hunt-Bark agar
Heart infusion agar (HIA) slants
0.1% Peptone diluent
Semi-solid medium : Neutral red (NR) solution, glycine, NaCl, cysteine HCl, KNO3
Triple sugar iron (TSI) agar slants
O-F glucose
MacConkey agar
Culture shipping media
Cary-Blair medium
Semi-solid medium, modified, for short-term culture storage
Culture freezing media
Hippurate and ninhydrin reagents
Nalidixic acid and cephalothin antibiotic disks
Hydrogen peroxide, 3%
Fetal bovine serum (FBS), filtered (0.22 μm)
Oxidase reagent, liquid type preferred
Gram stain reagents ; counterstain with 0.5% carbol fuchsin (Difco)
Nitrate detection reagents A and B
Lead acetate strips
Dryspot Campy Test
Sterile water, 1-2 liters; 70% ethanol or 1000 ppm hypochlorite solution

42°C

24-48 h
No. Klasifikasi
1 Method
2 Media

Reagen dan Stains

3 Suhu Inkubasi (⁰C)

Waktu Inkubasi
4 (Jam)
 BAM
Chapter 6
Shigella broth with novobiocin
Trypticase soy-yeast extract (TSYE) broth
MacConkey agar
Triple sugar iron (TSI) agar
Urea broth
Motility test medium (semisolid)
Potassium cyanide (KCN) broth
Malonate broth
Tryptone (tryptophane) broth, 1%
MR-VP broth
Christensen citrate agar
Veal infusion agar
Bromcresol purple broth
Acetate agar
Mucate broth
Mucate control broth
Decarboxylase basal medium (lysine, Falkow)
Decarboxylase basal medium (ornithine)
Kovacs' reagent
Voges-Proskauer test reagents
1 N Sodium hydroxide solution
1 N Hydrochloric acid
Methyl red indicator
Physiological saline solution, 0.85% (sterile)
Novobiocin
Polyvalent Shigella antisera for groups A, A1, B, C, C1, C2, D and Alkalescens-Dispar biotypes 1-
4
Gram stain reagents
35

20
 ISO
21567:2004
Shigella broth
Novobiocin solution
MacConkey agar
xylose lysine desoxycholate (XLD) agar
Hektoen enteric (HE) agar
Nutrient agar
Triple iron sugar (TSI) agar
Urea agar
Semi solid nutrient agar
Urea solution
Complete medium
Lysine decarboxylase medium
reagen for indole reaction
ẞ-Galactosidase reagent
Bromresol purple broth
Saline solution
Sodium acetate agar
Christenseen's citrate agar
Mucate broth
Control broth

37

20-24