Escolar Documentos
Profissional Documentos
Cultura Documentos
potency Reporters
Features Introduction
N
Higher viral titer than other anog, Oct4, and Rex1 promoters have been reported to display ES/iPS cell-
lentiviral vectors specific expression under undifferentiated state. This kit provides all three
Reliable expression levels promoter-fluorescent protein (FP) reporters to confirm the state of the reprogrammed
Self inactivating vectors iPSCs. A GFP or RFP gene was cloned behind these ES- specific promoters to offer
convenient fluorescence tracking of undifferentiated cells.
Lentiviral vector design and virus Lentiviral infection has advantages over other gene-delivery methods including:
packing techniques used to create high-efficiency infection of dividing and non-dividing cells, long- term stable
this product are covered by several expression of a transgenes, and low immunogenicity.
United States patents under agree-
ment to Allele Biotech, contact
vivec@allelebiotech.com or call 858-
587-6645 for details.
Pluripotency Promoter Reporter ABP-SC-ESRKIT1 each reporter 2 vials, 10^7TU in 1 vial
Panel(Nanog, Oct-4 and Rex1) 0.1ml
Nanog Promoter Reporter ABP-SC-RNANOG1 1 vial, 10^7TU in 0.1ml per vial
Nanog Promoter Reporter ABP-SC-RNANOG5 5 vials, 10^7TU in 0.1ml per vial
Website: www.allelebiotech.com
Oct-4 Promoter Reporter ABP-SC-ROCT4G1 1 vial, 10^7TU in 0.1ml per vial
Call: 1-800-991-RNAi/858-587-6645
(Pacific Time: 9:00AM~5:00PM) Oct-4 Promoter Reporter ABP-SC-ROCT4G5 5 vials, 10^7TU in 0.1ml per vial
Email: oligo@allelebiotech.com Rex1 Promoter Reporter ABP-SC-RREX2R1 1 vial, 10^7TU in 0.1ml per vial
Rex1 Promoter Reporter ABP-SC-RREX2R5 5 vials, 10^7TU in 0.1ml per vial
For Technical Support:
Email:iPS@allelebiotech.com
References
1. Da Yong WU, Zhen YAO (2005). Isolation and characterization of the murine
F or Research Use Only. Not for
Diagnostic or Therapeutic Use.
Purchase does not include or carry any
Nanog gene promoter. Cell Research, 15 (5): 317–324.
2. Rachel Eiges, Maya Schuldiner..et.al (2001). Establishment of human embryonic
stem cell-transfected clones carrying a marker for undifferentiated cell. Current
right to resell or transfer this product
either as a stand-alone product or as a Biology 11: 514–518.
component of another product. Any use 3.Guangjin Pan, Jun Li, Yali Zhou, Hui Zheng, and Duanqing pei (2006). A negative
of this product other than the permit-
ted use without the express written renewal. ASEB Journal 20: E1094~E1102
authorization of Allele Biotech is strictly
prohibited Note: The specificity of these promoters are based on scientific publications, which
are subject to debate.
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4-In-One-Vector (OSMK) Set: Nanog, Oct-4 and Rex1
2-In-One-Vector (OS) Any individual promoter in the set
Set: OSKM iPSCs Identification
Set: OSKMP Human iPS RT-PCR Primer Set (50 reactions for
Set: OSKMPT each of 45 genes)
Set: OSNL Mouse iPS RT-PCR Primer Set (50 reactions for
Set: OSNLP each of 29 genes)
Set: OSNLPT Antibodies (Check online)
Pre-Mix: OSNL Irradiated bFGF/LIF Producing Feeder Cells
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M: c-Myc; N: Nanog; L: Lin28 Irradiated bFGF-Producing MEFs
T: Tert; P: p53 shRNA Irradiated bFGF-LIF-Producing MEFs
Any individual gene in the set