Escolar Documentos
Profissional Documentos
Cultura Documentos
https://www.researchgate.net/publication/257181175
CITATIONS READS
17 1,246
3 authors, including:
Some of the authors of this publication are also working on these related projects:
All content following this page was uploaded by Eliana Gonzales-Vigil on 10 June 2016.
Publish by Abstract
Abstract. A method to extract high-quality DNA from potato tubers was developed and
tested on 3 wild potato species (Solanum raphanifolium, S. megistracrolobum, S.
bukasovii) and on the tetraploid B3 bred population (population number 393228, derived
from S. tuberosum subsp, tuberosum). The average yield of extracted DNA varied from
10-30 Ixg of DNA per gram of processed tissue. The DNA was pure and suitable for liga-
tion-mediated polymerase chain reaction (LM-PCR) amplification, producing clear, dis-
tinctive, and reproducible banding patterns in polyacrylamide gels.
Full text*: This manuscript, in detail, is available only in the electronic version of
the Plant Molecular Biology Reporter.
Contents: This manuscript contains: Introduction, Materials and Methods, Results and
Discussion, 3 references, and 5 illustrations.
Illustrations:
Table 1. Average of D N A yields obtained from tuber tissue of Solanum spp.
with the present protocol.
Figure 1. D N A extraction of wild Solanum species (S. raphanifoIium).
Figure 2. D N A extraction of wild Solanum species (S. megistracrolobum).
Figure 3. Ligation-mediated PCR amplimers on a 5% polyacrylamide gel.
Figure 4. Ligation-mediated PCR amplimers on a 5% polyacrylamide gel.
Key words: DNA extraction, LM-PCR, potato, Solanum, tuber