LEUKOPOIESIS

CFU-GM

CFU-M
Monoblast
Promonocyte
Monocyte
Macrophage
MONOBLAST
 ________________
 12-20 µm
 Basophilic cytoplasm
 ________________
 NC ratio 4:1-3:1
 1-2 nucleoli
PROMONOCYTE
- 12 to 18 µm in diameter
- chromatin pattern is delicate,
- at least one nucleolus is apparent
- Cytoplasm is blue ____________________
MONOCYTE
 15 to 20 µm
 Larger than neutrophil
 _______________________
 Nucleus:
____________________________________
 Chromatin: _____________
 Cytoplasm: __________________________
 ___________of circulating leukocytes, with an
absolute number of up to _______________
Monocytes
 Stays in PB for _______then move to tses to
become macrophage and probably not to
return
 _____________________for months or
years
 _______________________________
MONOCYTE/MACROPHAGE KINETICS

 Promonocytes undergoes _______________=

_________________
 ______________________in the bone marrow
 marginal pool of monocytes ________________
 remain in the circulation __________________
 MACROPHAGES
 large as _________in diameter
 Nucleus: oval with reticulated chromatin pattern
 Cytoplasm: pale, vacuolated, and often
____________________________
 Life span in tissues:
 Resident macrophages =
_______________________________
 inflammatory macrophages = ________________
FUNCTIONS OF MONO&MACRO
1. INNATE IMMUNITY
 Pattern recognition receptors  __________

__________________________

 Macrophages = ______________

 Fc receptors = __________________________
___________________________
FUNCTIONS OF MONO&MACRO
2. ADAPTIVE IMMUNITY
 Ag presenting cells – _____________________

 Dendritic cells – ________________________

___________________
FUNCTIONS OF MONO&MACRO
2. Housekeeping Functions
 Removal of ______________

 Destruction of _______________

 Maintains ___________________

 Synthesis of ________________
LEUKOPOIESIS

T cell B cell NK Cell

Pro-T cell Pro-B cell

Pre-T cell
Pre-B cell
T cell
B cell

NK cell-recognize and lyse Tumor cells

Plasma Cell- secrete Igs
ADAPTIVE IMMUNITY
 relies on enormous number of distinct
lymphocytes
 Production of ___________
 ___________
 LYMPHOCYTES
 _______of circulating leukocytes with absolute
no. of ______________

2 Major Categories
 Humoral immunity = _______________

 Cellular immunity = _______________________

LYMPHOCYTES
 Not _________
 Able to recirculate
 Capable of _________________
 T and NK cells develop and mature
_____________
LYMPHOPOIESIS
 Lymphoid tissues: BM, thymus, LN, spleen,
tonsils, Peyer’s patches , foci of
subendothelial and subepithelial
lymphocytes, monocytes and macrophages.
 Primary Lymphoid Organs(PLO)
 Secondary Lymphoid Tissue(SLT)
B LYMPHOCYTES
 PRO-B, PRE-B, and IMMATURE B
 Gene rearrangement
 Morph not established
 Immature B cells = leave the BM and migrates
to SLO ________________
- usually seen in ________________
- similar to cells in ___________
- ___________________________
= ______________________________
 Resting B and T cells can’t be distinguished
B LYMPHOCYTES
 ___________of circulating lymphocytes

FUNCTION
 ______________
 Ag presentation _________
 Necessary for ____________
 Produce _________
PLASMA CELLS
 Deeply basophilic cytoplasm
 ________________
 Eccentric nucleus
 Condensed and coarse chromatin
 Nucleoli not visible
T LYMPHOCYTES
 _________
 Progenitor cells = BM  thymic cortex
 PRO-T, PRE-T, and IMMATURE T CELLS
 Undergo rearrangement = _________________
 2 major categories:
______________________________________
 Immature T cells  ________________
 Remaining immat T cell  _______ =
__________________________________
T LYMPHOCYTES

 ______________of circulating lymphocytes.

FUNCTION
 CD4
 ______ = against intracellular pathogens
 ______ = against extracellular parasites, induction
of asthma and other allergies
 ______ = against extracellular bacteria and fungi
 ______ = maintaining self tolerance by regulating
immune response
T LYMPHOCYTES
FUNCTION
 CD8 – secrete granules with
______________________________
- activate ______________on target
cells

- _______________________
NK CELLS
 _______________
 Heterogeneous grp of cells with respect to
their surface Ag
 Majority: ____. ______, ______ and ______
 Mature cell – relatively large compared with
other resting lymph
 _______________
 Killing certain tumor cells and virus infected
cells ____________________
 ____________of circulating lymphocytes
LYMPHOCYTES
 Major role in maintaining health and in response to and
recovery from disease
 Not obligate end cells- continuous supply of inc
differentiated cells then transform
 Heterogenous group of cells- Tcells, Bcells,NK cells,
non-T and non-B cells
 Predestined to migrate- other cells (one direction
migration) but both T and B cells freely migrate to and
from body tissues
 Lifespan- short lived B cells (3-4 days), long lived T cells
(average 4 yrs some up to 20 yrs)