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Iqbal Hussain, Atta U. Khan, Riaz Ullah, Mansour S. Alsaid, Syed Salman,
Sarwat Iftikhar, Gul A. Marwat, Muhammad Sadique, Samin Jan,
Muhammad Adnan & Akash Tariq
To cite this article: Iqbal Hussain, Atta U. Khan, Riaz Ullah, Mansour S. Alsaid, Syed Salman,
Sarwat Iftikhar, Gul A. Marwat, Muhammad Sadique, Samin Jan, Muhammad Adnan & Akash
Tariq (2017) Chemical Composition, Antioxidant and Anti-bacterial Potential of Essential Oil of
Medicinal plant Isodon rugosus, Journal of Essential Oil Bearing Plants, 20:6, 1607-1613, DOI:
10.1080/0972060X.2017.1407677
Iqbal Hussain 1, Atta U. Khan 1, Riaz Ullah 2*, Mansour S. Alsaid 2, Syed
Salman 1, Sarwat Iftikhar 3, Gul A. Marwat 4, Muhammad Sadique 4,
Samin Jan 3, Muhammad Adnan 5, Akash Tariq 6,7
1
Department of Chemistry, Islamia College Peshawar, KPK, Pakistan
2
Department of Pharmacognosy and Medicinal, Aromatic & Poisonous Plants Research Center,
College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
3
Department of Botany, Islamia College Peshawar, KPK, Pakistan
4
Medicinal Botanic Center, PCSIR Labs Complex Jamrud Road Peshawar KPP Pakistan
5
Department of Botany, Kohat University of Science and Technology KUST KPK Pakistan
6
CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization &
Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan
Province, Chengdu Institute of Biology, Chinese Academy of
Sciences, Chengdu 610041, People’s Republic of China
7
University of Chinese Academy of Sciences, Beijing 100093, People’s Republic of China
Received 22 August 2017; accepted in revised form 17 November 2017
Abstract: Isodon rugosus is a medicinally valuable herb containing a variety of important secondary
metabolites and essential oil with important chemical constituents of pharmaceutical precursors. The active
components of volatile oil of I. rugosus were analyzed using GC-MS. Anti-oxidant potential of the oil was
assessed with 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Anti-bacterial power of the essential oil accessed disc
diffused method. A total of 19 constituents were quantified and identified with different concentrations in
essential oil. Among which Caryophyllene oxide was 28.99 %. Limonene oxide cis was 26.52 %, (Z, E)-Farnesol
was 17.34 %, followed by 1-erpinen-4-ol 3.33 %, o-Cymene 2.04 while the concentrations of rest of constituents
were between 1.11 to 0.2 %. The investigation showed that the essential oil of I. rugosus possessed significant
antibacterial and antioxidant potential
90
80
% DPPH scavenged
70
60
50 essential oil
40 Standard
30
20
10
0
10 20 30 40 50 60
Concentration (µg/mL)
Fig. 1. Antioxidant activity of essential oil of I. rugosus
Iqbal Hussain et al., / TEOP 20 (6) 2017 1607 - 1613 1612
Conclusion Acknowledgment
The analytical data of essential oil and its The authors are thankful to the support of
biological potential shows that this plant is a good research Center, College of Pharmacy and
source of vital essential oil components which Deanship of Scientific Research at King Saud
may play vital applications in the maintenance University Riyadh, KSA.
of human heath particularly in the skin, cosmetics
and against human pathogens if discovered Conflict of Interest
further. Authors declare no conflict of interest.
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