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HLA Complex
HLA Class I HLA Class II
Peptide Binding Cleft
Cell Membrane
TAP1
DQA1
DPA1
DQB1
DRB3
TAP2
DRB5
MICB
DMB
LMP2
LMP7
TNF
DOB
DMA
DRB4
DOA
MICA
Bf
C2
DRA
C4
Nomenclature
Hyphen used to SUFFIX EXPRESSION STATUS
separate gene name Field
from HLA prefix Separator Separators
L Low cell surface expression when compared to normal levels.
HL A- DRB4*01:03:01:02N S Expressed as a soluble, secreted molecule but is not present on the cell surface.
HLA Prefix Gene Field 1 Field 3 Suffix C Alleles that produce proteins that are present in the cytoplasm and not on the cell surface.
Allele Group used to show used to
a synonymous denote
DNA substitution changes in
A Aberrant expression where there is some doubt as to whether a protein is actually expressed.
within the expression
coding region
Q Expression of an allele is questionable, given that the mutation seen in the allele has
Field 2 Field 4 been shown to affect normal expression levels in other alleles.
Specific used to show
HLA Protein differences in
a non-coding
region N Alleles that have been shown not to be expressed (null).
Determine DNA-Based HLA Typing • Sequence-Specific Oligonucleotide Probes (SSOP) NO Not Applicable
HLA Type • Sequence-Specific Primer (SSP)
• Next Generation Sequencing (NGS)
Detect Donor Patient-Donor Crossmatch • Flow Cytometry Crossmatch (FCXM) Assay YES Total Lymphocytes: 19655, 19961HLA
Specific Antibodies • Complement-Dependent Cytotoxicity (CDC) T Cells: 19671, 896712
(DSA) Crossmatch Assay B Cells: 19684, 896842
Reference: 1. Howell WM et al. (2010) J Clin Pathol 63(5): 387–90. | 2. Lima-Junior J da C & Pratt-Riccio LR. (2016) Front Immunol 7: 13. | 3. Marsh SGE et al. (2010) Tissue Antigens 75(4): 291–455. | 4. Robinson J et al. (2015) Nucleic Acids Res 43 (Database issue): D423-31. | 5. Sasazuki T et al. (2016) Adv Immunol 129: 175–249. |
6. Tinckam KJ. (2012) In: Chandraker A, MH Sayegh, AK Singh (Eds.) Core Concepts in Renal Transplantation. Boston, MA: Springer US.: pp21–42. | 7. Zhang GL et al. (2014) Front Immunol 5: 597.
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