Lab Water

Product Training
Agenda

Water Contaminants
Water Requirements
Purification Technology Overview
Millipore Lab Water System
 DI water Aquadest Aquademin

Water for injection RO Water

Purified water Aquabidest

Highly Purified water DNase Free water
ATP Free water Pyrogen Free water
Sterile water Rnase Free water
Protease Free water
Water applications in Laboratory
Animal Watering  GC MS  Microbiological Media
Aquariums  GFAA  North. & South. Blotting
Atomic Absorption  Glassware Washing  Nuclease-Free Appl.
Autoclaves  Heating Baths  Nucl. Ac. Electrophoresis
BOD  Histology  Particle Analyzers
Capillary Electrophoresis  HPLC  RT PCR
Cell Culture  Hydrogen Generators  pH Measurement
Chemiluminescence  Hydroponics  Protein Electrophoresis
Clinical & Biomedical  IC  Pyrogen-free Appl.
COD  ICP-MS  Spectrophotometry
DHPLC  ICP-OES  Stability Testing
Dissolution Testing  Immunohistochemistry  Titrators
DNA Sequencing  In vitro Fertilization (IVF)  TOC analysis
DNA Microarrays  Kjeldahl Analysis  UHPLC
Electrophysiology  LC-MS  VOC
Endocrine Disrupters  Lithography  Weatherometers
Fluorescence  MALDI-ToF  Western Blotting
The role of water in analytical chemistry

Mobile phase Preparation of standards

Analytical Method

Washing, rinsing Blanks

of sample & reagent
containers
Sample preparation
Measurement of Contaminants

Contaminants Measurement Unit

Inorganic Ions Conductivity μs/cm
(Resistivity) MΩ.cm
Organics Total Oxidizable ppb (μg/L)
Carbon (T.O.C.)
Particles (Colloids) Silt Density Index / Rate of pluggage of
Fouling Index 0.45 μm membrane.
Bacteria Colony count on 0.45 μm cfu/ml
Membrane.
Endotoxin Rabbit Inoculation test Endotoxin units/ml
LAL Test
Conductivity / Resistivity Computing

 Conductivity :

 F  Ci Zi µi
= 

Conductivity Mobility
Concentration of Valence
(µSiemens/cm) V-1.cm2.s-1
each ionic species
(moles/mL)
Faraday

 Resistivity : the inverse of the conductivity

R = 1/ Resistivity
(MOhm.cm)
 Pure Water Resistivity = f (T°C)
 Theoretically pure water ions = only H+ + OH- (
water dissociation) :
Resistivity [ H+ ] = [ OH- ] = 10-7 mol / L
100
(M.cm)
90 ⇒ high electric resistance

80

70  At 25°C, pure water conductivity / resistivity :

60  = 0.055 S/cm
50 R = 18.18 M.cm
40

30
 An electric current can go through water because
20 18.18
of contaminant ions (mainly).
10

0
0 10 20 30 40 50 60 70 80 90 100

Temperature (°C)
Why test for TOC ?

Quality & Reliability of scientific data depends on the

purity of the reagents used
Monitoring is required to warrant reagent’s purity
Water is the most commonly used reagent ; so far only the
ionic contamination has been monitored through
resistivity
Some laboratory applications are TOC sensitive (HPLC,
UPLC, ICP, LC, etc)
International norms and standards require low TOC values
for ultrapure water.
Agenda

Water Contaminants
Water Requirements
Purification Technology Overview
Millipore Lab Water System
Standard Requirements
Standard specifications
 Explicit set of requirements for an item, material, component, system or service

Water standard requirements

 Several water standards are coexisting depending on the
– USP/EP: Purified Water, WFI (& Highly purified water)
– CLSI Reagent Water
– ASTM D1193 : Standard Specification for Reagent Water
– ISO 3696 : Water for analytical laboratory use

Guidelines
 Framework of common industry practices and recipes
– ISPE (International Society for Pharmaceutical Engineering)
– PIC/S pharmaceutical inspection convention/cooperation scheme
– CLSI - Clinical and laboratory Standard Institute
US and European Pharmacopoeia
CLSI* water quality specifications

Water qualities defined by the CLSI:

**Clinical laboratory Reagent Water (CLRW)

 CLRW Quantitative Specifications: Resistivity: ≥ 10 MΩ.cm referenced to 25° C; Total
Heterotrophic Plate Count: < 10 cfu/mL; TOC: < 500 ng/g (ppb); Particulates: puridication
stage that blocks the passage of particles ≥ 0.22 μm at or near the output stage.

Special Reagent Water (SRW)

Instrument Feed Water (IFW)
Water Supplied by a method manufacturer for use as a Diluent or Reagent
Commercial bottled, Purified Water
Autoclave and Wash Water Applications

*CLSI: Clinical and Laboratory Standards Institute (previously NCCLS)

** Concerning CLSI Water Qualities, complete CLSI corresponding sections should be read in order to understand the philosophy, method,
limitations, recommendations for each requirement.
13
ISO® (International Organization for Standardization)
ASTM (American Society for Testing and Materials)
Agenda

Water Contaminants
Water Requirements
Purification Technology Overview
Millipore Lab Water System
Contaminant Removal Efficiency
Lab Water Purification Technologies

MILLIPORE uses 6 different technologies :

Ion-Exchange
Reverse Osmosis
Filtration
Ultrafiltration
Activated Carbon Adsorption
UV Radiations
Vacuum Degassing
 Ultrapure water
Millipore Proposed Specifications

Contaminant Parameter (unit) Type III Type II Type I

Ions Resistivity (M.cm) > 0.05 > 1.0 > 18.0

TOC (ppb) < 200 < 50 < 10
Organics Pyrogens (Eu / mL) NA < 0.25 < 0.03
Particulates >
Particulates 0.2µm (units / mL)
NA NA <1

Colloids Silica (ppb) < 1000 < 100 < 10

Bacteria Bacteria (cfu / mL) < 1000 < 100 <1

Standards and Common Terms
Ultrapure/Reagent Grade Critical Applications
Water for HPLC,GC, UHPLC ,AA , ICP-MS, for buffers and
culture media for mammalian cell culture & IVF, reagents for
molecular biology...
Milli-Q, Super-Q, --- AFS, Direct-Q3, ----- Milli Q Integral/Direct

“Ultrapure” Type 1
Synergy & Simplicity

Pure/Analytical Grade Standard

Applications
Buffers, pH solutions,culture media preparation ,clinical
analysers and weatherometers feed.
Small & large Elix, ---- RiOs DI, ------ Milli-DI
Type II
“Pure” Pure/Laboratory Grade
General Applications
Glassware rinsing, heating
baths, humidifiers and
autoclaves filling
Type III Small & large RiOs
 Lab Water purification systems :
An optimized assembly of water purification & built-in monitoring technologies to
address our customers needs.
Polishing Step POU
Pretreatment EDI Storage
purifiers
step process
Reservoir
Ion Exchange resins +
UV254nm Activated Carbon
Pretreatment
pack Electrodeionization

Reverse Osmosis

Tap UV185/254nm
UV254nm
water

Pure
water Ultrapure
water

21 Basic Lab Water Training – Indonesia - Apr

Agenda

Water Contaminants
Water Requirements
Purification Technology Overview
Millipore Lab Water System
 Water
Types

Tap
Type 3
Type 2
Type 1

1
Simplicity

5
RiOs 3

RiOs-Di 3

Milli-DI
Milli-Di

Direct-Q 3

Direct-Q 5

Synergy

25
RiOs 5 -16

Elix 3 - 15

Milli-Q Reference & Adv.

Milli-Q Integral 3 - 15
250

RiOs Large
Millipore Lab Water System Panorama

Elix Large

Super-Q
Volume in L / day
Water Purification Systems
Tap Water to Tipe 2 & Tipe 3

RiOs (Smart) – 3 to 8 litres/hr of Type III water

RiOs Essential – 5 to 24 litres/hr of Type III water

Elix Essential – 3 to 15 litres/hr of Type II water

Elix Advantage– 3 to 15 litres/hr of Type II water

Low to Mid Volume Pure Water Systems

RiOs (Smart) 3/5/8

RiOs
Essential Elix Essential Elix Advantage
5/8/16/24 3/5/10/15 3/5/10/15
Feed water > limit specifications ?

Water Quality Potable tap water

Conductivity < 2000 µS/cm
Temperature 5 - 35 °C
Pressure range 1 - 6 bar
pH 4 - 10
Fouling index <12
Free Chlorine <3ppm
Langelier saturation index <0.3

Launch
25 RiOs Essential | May 2012
Water Purification Systems
Ultrapure Water Systems – Pure Water Feed
Pure Water Feed Tipe 2/3 to Ultrapure Tipe 1

Synergy and Simplicity

 Part of the Smart range of model.

Synergy with Simplicity
remote Dispenser
 Option of UV and remote
Dispenser

 Ultrapure water for lower volume

requirements (<10 litres/day).

 Designed for installation by

customers.

 Ideal for current bottled water Synergy with remote Dispenser Simplicity
users.
Ultrapure Water Quality
 Simplicity : Applications

Titrators, Buffers, pH
solutions, Standards

Chromatography:
HPLC, ILC, LC
Simplicity
Simfilter
Cell Culture

Molecular Biology
(PCR, DNA
sequence, Blotting)
Simplicity UV BioPak
Water Purification Systems
Ultrapure Water Systems
Pure Water Feed Tipe 2/3 to Ultrapure Tipe 1

Milli-Q Reference

 General use ultrapure system.

 Medium to high volume

requirement.

Option of TOC Indicator and remote

dispensing

 Ideal for academia.

Milli-Q Reference  High ultrapure requirements, non-

critical.
Milli-Q Reference
Water Purification Systems
Ultrapure Water Systems
Pure Water Feed Tipe 2/3 to Ultrapure Tipe 1

Milli-Q Advantage A10

 Top of the range ultrapure.

 Medium to high volume

requirement.

 Ultimate flexibility and monitoring.

 For critical ultrapure water

requirement.

A10 refers to TOC monitor.

Milli-Q Advantage A10

Milli-Q Advantage A10

Water Purification Systems
Ultrapure Water Systems
Pure Water Feed Tipe 2/3 to Ultrapure Tipe 1

Smart Range – 2 to 10 litres per day of Type I water at 0.5 to

1.5 litres/min flowrate.

Synergy with Simplicity

remote Dispenser

Milli-Q Reference – up to 100 litres per day of Type I water at

2.0 litres/min flowrate for academic use.

Milli-Q Reference

Milli-Q Advantage A10 – up to 100 litres per day of Type I

water at 2.0 litres/min flowrate for critical use.

Milli-Q Advantage A10

Water Purification Systems
Tap to ultrapure water Tipe 1 & Tipe 3

Direct-Q 3/5/8

 Part of the Smart range model.

Option of UV and remote dispensing.

Direct-Q 3/5/8 UV with
remote dispenser
 Ultrapure water for low volume
requirement (<10 litres/day).

 Designed for installation by

customers.

 Ideal for current bottled water users .

Direct-Q 3/5/8 UV with remote dispenser

Pure & Ultrapure Water Quality

Sale 33
s
Water Purification Systems
Tap to ultrapure water Tipe 1 & Tipe 3

Milli-Q Direct 8/16

 Type 3 and Type 1 water.

 Medium to high volume requirement.

 Ideal for academia.

 High ultrapure requirements, non-

critical.

With TOC Indicator

Milli-Q Direct 8/16

Milli-Q Direct 8/16

Water Purification Systems
Tap to ultrapure water Tipe 1 & Tipe 2

Milli-Q Integral A10

• Top of the range tap to

ultrapure.

•Type 2 and Type 1 water.

• Medium to high volume

requirement.

• Ultimate flexibility and

monitoring.

• For critical ultrapure water

requirement.

•TOC monitor Milli-Q Integral A10 3/5/10/15

Milli-Q
Integral A10
3/5/10/15
Water Purification Systems
Tap to ultrapure water

• Direct-Q 3/5/8 (Smart) – 3 to 8 litres per hour of Type III

Direct-Q 3/5/8 UV with
water, 6 litre internal storage reservoir or 30/60 litre
remote dispenser bench top reservoir, and >1.5 litres/min of Type I
water.

• Milli-Q Direct – 8 or 16 litres per hour of Type III water,

external reservoir and 2.0 litres/min of Type I water.
Milli-Q Direct 8/16

• Milli-Q Integral – 3 to 15 litres per hour of Type II water,

external reservoir and 2.0 litres/min of Type I water.

Milli-Q
Integral A10
3/5/10/15
 POD paks
PAK: MilliPak 40 BioPak LC - Pak VOC - Pak EDS - Pak

Biological Contaminants
Particulate removal and Removal; RNase,
Removal of Trace Organic Removal of Volatile Removal of Endocrine
Purpose: Bacteria removal DNase, Pyrogens,
Compounds Organic Compounds Disrupters
(LRV > 7) Proteases, Viruses and
Bacteria

Endocrine Disrupter
General laboratory Cell Culture, Trace organics analysis by
Research, Endocrine
Application: applications, Analytical Biochemistry, Molecular HPLC, UPLC, LC-MS, LC- Water Analysis by GC
Disrupter Screening and
applications Biology MS/MS
Testing

HPLC Gradient Test - Absorbance

of highest eluted peak
Removal of:
Bacteria < 0.1 cfu/mL  Bisphenol A
Pyrogens/ < 0.001 Eu/mL At 210 nm < 0.003 AU
At 254 nm < 0.001 AU  Diethyl Phthalate
Endotoxins Refer to Brochure for
Specification: Bacteria < 0.1 cfu/mL  Di-n-butyl
RNases < 0.01 ng/mL Compliance with suitability for detailed specification Phthalate
DNases < 4 pg/mL LC/MS: Reserpine test  Nonylphenol
No peak higher than 10 ppb  Other EDS
Reserpine at 609 m/z in ESI +
Conclusion
PRODUCT WATER
NO SOURCE WATER SYSTEM
TYPE 3 TYPE 2 TYPE 1
DIRECT 3, 5, 8
MILLI Q DIRECT 8, 16
MILLI Q INTEGRAL 3,5,10,15
RiOs 3,5,8
1 GROUND WATER/PDAM RiOs ESSENTIAL 5,8,16,24
RiOS DI
ELIX ESSENTIAL 3,5,10,15
ELIX REFERENCE 3,5,10,15
ELIX ADVANTAGE 3,5,10,15
SIMPLICITY
SYNERGY
2 RO / DESTILAT WATER MILLI Q REFERENCE
MILLI Q REFERENCE A+
MILLI Q ADVANTAGE A10
We can offer both solutions….

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Bench Integration by Design
USA – 2012-05-04
Wall Mounted
US Installations 2012-03-31
Underbench
Flexible installation options
Questions ?
“Water is a chemical reagent”

Thank you for your attention !!