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UV-2550
2550 SPECTROPHOTOMETER
TEL (310)202-0814
(310)202 FAX (310)202-7286
Email: spectro@labomed.com
Preface
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Content s
Preface................................................................................................................................ 2
Contents ........................................................................................................................... 3
3.2 . Con nect ion of th e inst rument .................... Error! Bookmark not defined.
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Chapter1 Software Functions
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Chapter 2 Installation and uninstallation of the Software
,
1. “Start”--“Control Panel” Double click “Add/Remove Programs”;
,
2. Find the program to be deleted select it and click “Remove”. Uninstall it
according to the prompts.
Or click “Start” ---- “All programs” to find the “UVSoftware”, select “Uninstall
UVSoftware” to delete the software.
5
Chapter 3 Initial Setting and Online Testing
5.Click 'settings' to select correct Com port and input the right serial number.
7.Single c lick O n the tool bar m enu, enter the photom etr ic m easur em ent.
Press and hold “PRINT” key when the spectrophotometer is off. Then turn on
▌K E Y P G A S N R S T
V E R : 1 . 1 0
Press “100%” key or “0%” key to move the cursor to the front of “SN” and
press “ENTER” key. The following will be shown. Note down the SN. Such as
00090351
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▌E D I T
S N : 0 0 0 9 0 3 5 1
XP)
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3) Click “Device
Manager”
button to display the following window.
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Click at item “Ports (COM & LTP)” and find item “Silicon Labs…..(COM?)”
as shown in the above figure. The COM port number (as “COM3” in the
figure) is displayed. This port number should be input later as the
communication port No. in the spectrophotometer PC control software
“UVSoftware”.
Double click the software icon to enter the software interface (for Windows
XP system, just double click to run software; for Windows Vista system, right
click mouse, then select “run as administrator” to run software, if not do so,
com port will not be able to select).
Click “Settings” to select the com port and input the serial number.
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3.1. Co mmunicatio n port setting
Fig. 1
If test succeed, the following information will appear.
Fig. 2
Then click “OK”, serial number of instrument will appear at lower right corner,
see in Fig.3. If connection succeeds during connect test, then operator do
not need to click “Connect” again. Besides, once connection is success,
system will save the previous serial number and comport as default, then
operator do not need to input them again.
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Fig. 3
Fig. 4
Single cl ick on the tool bar menu, e nter the
photometric measurement.
Note: now you can complete all the measure ment step s just by
PC software, I me an all t he steps c an be controlled totally b y
PC. So, Plea se don’ t touch any key on the spectro.
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Chapter 4 Functions and Applications of the software
Fig. 5
In this interface:
Photometric measurement
Kinetics measurement
Quantitative measurement
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Parameter settings
4.1.1. Single click on the tool bar menu, enter the photometric
measurement, shown as in Fig.6.
Fig. 6
Measurement start
Stop measurement
Parameters setting
Save data
Print report
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4.1.2. Parameters setting
Single click ,
on the tool bar menu set the parameters, shown as in
Fig.7.
Fig. 7
Select the data mode: Abs or T%, select the decimals and modify the
wavelength. After all parameters setting well, click OK.
Reference Test:
Once, test reference once, and after a measurement
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Wavelength
Add the wavelength, according to customer’s need,
single click it can increase the wavelength.
Delete the wavelength, single lick it can delete the no
necessary wavelength.
Single click it can modify the wavelength.
Clear off, single click it can clear off all of the
wavelengths
Fig. 8
Notice:
When setting wavelength, same wavelength can not be set, if else when
click “OK” button, system will inform you as follow:
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Fig. 9
Considering the limitation of report printing, number of wavelength added or
formula added shall not more than 7, if yes, system will warn you with the
following window,
Fig. 10
Then operator needs to delete some wavelength or formula, till the number
of wavelength or formula less than 7.
Formula is to calculate the measurement result, so the formula shall be input
according to the number of wavelength, if not, system will deny operation.
For example, 2 wavelengths are measured, then in the formula, only A and B
are valid, C, D or any other number of wavelengths shall not appear in the
formula.
4.1.3. Measurement
Put the reference and sample into sample cells, single click on the
tool bar menu, start the measurement, cueing the wavelength should be set
to 650nm, see Fig. 11, turn around the wavelength button and set the
wavelength to 650nm, then click OK.
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Fig. 11
According to the cue in Fig. 12, place the reference in light path and click
OK.
Fig. 12
According to the following cueing, see Fig.13, place the light block in light
path, and then click OK.
Fig. 13
According to the following cueing, Fig 14, place sample in light path, and
then click OK。
Fig. 14
Now the Abs or T% measurement at 650nm is completed. The cue appears,
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see Fig.15. If customer wants to measure Abs or T% of more wavelengths,
only need to add the wavelength, and operate as above procedures.
Fig. 15
Notice:
When instrument is measuring, user clicks “Measure”, “Wavelength locating”
or other function buttons, system will inform as follow. Other operation shall
be done after measurement complete.
If instrument is not initialized, then user click any function button, user will get
information as follow.
finish test.
After test finish, if a new test is needed, it is not necessary to quit test window,
then click “OK” button, system will create a new test page.
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During the process of zeroing or testing, if user presses button to stop
without quitting the test window, system will clear all the current data. Click
page. If test mode, wavelength, reference test mode is changed, system will
rerun zero and then start new test; others doesn’t require re-zero.
When shutting off test page, user will get following information,
Select “Yes” to remain in test window, and user can save the test result
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4.2. Kinetic s measurement
4.2.1. Single click the on the tool bar menu, enter the kinetics
measurement mode, shown as in Fig.17.
Fig. 17
Start measurement
Stop measurement
Parameters setting
Save data
Spectrum calculation
+ -, ×, ÷)
( ,
Peak & valley picking
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Spectrum conversion
Cursor
Print report
4.2.2. Parameters setting
Single click on the tool bar menu, the following window will come
out, see Fig. 18.
Fig. 18
In this window,:
Reference Test: Once, after test complete, do not change any parameter,
click button to continue test will test sample directly,
Save mode: Auto Save, after test, system will save automatically in the folder
that user set, if scan mode is Repeat, name of saved file will be
added “_1”, “_2” etc.; Manual, system will ask if you want to save
result after a test; Not Save, system will not ask you to save result.
OverLay: by selecting this, all tested result will be shown on Data page, you
can also use this function to see certain result on Data page
manually.
Under this interface, you can select data mode: Abs, T% or Energy. Also you
can set wavelength range and photometric range (display range), sampling
interval, scan speed, reference test mode and decimals. After all parameter
has set, click “OK”.
System will create automatically a new page Data1, and update the
coordinates of spectrum (see 1 in Fig.19), the parameters that are set will be
shown at right part (see 2 in Fig.19), test result will also be displayed at right
part (see 3 in Fig.19), and current wavelength will be shown at the top of
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Fig. 19
4.2.3. Measurement
Separately place the reference and sample into cells, single click
on the tool bar menu, start the measurement, according to the cueing, see
Fig.20, turn the wavelength button, set the wavelength to 650nm, then click
OK.
Fig. 20
According to the cueing, see Fig. 21, place the reference in light path, and
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click OK.
Fig. 21
According to the cueing, see Fig. 22, place the light block, then click OK.
Fig.22
According to the cueing, see Fig. 23, place the sample in light path, and
click OK, now the data can be obtained.
Fig. 23
After measurement finish, system will inform user as Fig. 24,
Fig. 24
Click “OK”, test result and its spectrum will come out, the spectrum is as Fig.
25.
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Fig. 25
Repeat measure, this test can be done manually, after a single measure is
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Fig. 26
If user select scan mode to be “Auto”, see Fig. 27, then set time interval, this
indicates system will measure every 3 seconds, till user click button.
Fig. 27
Notice:
When instrument is measuring, user clicks “Measure”, “Wavelength locating”
or other function buttons, system will inform as follow. Other operation shall
be done after measurement complete.
If instrument is not initialized, then user click any function button, user will get
information as follow.
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Generally, Press to start Repeat test after Once test, or select
Repeat test and set its parameter to start repeat test. It would be better not
if one does not escape this test window, system will clear all current data
that is tested; if one click again, system will restart the test.
After a test, change any of the parameters, a new test window will created.
When shutting off test window, system will inform you as Fig.28 shows. Select
Y, system will stay on test window, user can save data according to their
demand; if select N, system will quit directly.
Fig. 28
4.2.4 Additional function
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a. Spectrum adjustment
After measurement, right click mouse on spectrum, you will find the following
menu:
In witch:
Fig. 29
Channel Color ---- set channel color for data page. See Fig.30, double click
color button, color select window will appear, select the color you want,
and click “OK”.
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Fig. 30
Overlay ---- Spectrum overlay can show all the test spectrum on one data
page, see Fig.31, select spectrum you want to overlay (in Fig.31
Da_0 means property of page, 0~10s indicates scan time, 1
means time interval), click “OK”. The overlay spectrum that you
selected will be shown in Data page, see Fig.32.
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Fig. 31
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Fig.32
By selecting from the marked toolbar in Fig.32 can display certain spectrum;
if select Show all, all spectrum will be showed.
b. Cursor
Click button on tool bar, vertical cursor will be displayed, you can
observe data by moving your mouse, also you can use “←” & “→” to move
cursor slowly. If you need to move cursor fast, press “Shift+←” & “Shift+→”.
c. Peak picking
Select the spectrum that you want to pick peak, click button
on tool bar, peak picking window will come out (this function is used for
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certain spectrum, not for overlay spectrum), see as in Fig. 33, then input
threshold and click “OK”, system will mark peaks and valleys on spectrum,
Fig. 33
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Fig. 34
If system did not find any peak because of improper threshold, system will
inform you as Fig. 35.
Fig. 35
When doing Peak Picking, if you stay in Data page (Overlay page), system
will inform you to select the data you want to do peak picking, see the
Fig.36.
Fig. 36
d. Spectrum transfer
and click “OK”. But for “Derivative”, do not forget to select∆λ and order of
derivative.
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Select spectrum you want to transfer, click button on tool
bar then spectrum conversion window will come out, see as shown in Fig. 26.
Select the function you need, and click “OK” button, then system will show
click “OK” button, the changed spectrum will become as what we can see
Fig.37
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Fig. 38
If you do spectrum conversion on Data page (overlay page), system will
inform you as Fig.39 shows. Then please select a certain page to do this
conversion.
Fig. 39
e. Arithmetic Function
Fig. 41
But if the wavelength range, time interval and scan mode of spectrum you
selected are different, arithmetic function will not work. System will inform
you as follow.
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Fig. 42
Spectrum copy, right click mouse on spectrum, select “Copy”, then you can
paste the spectrum into Word or Excel file.
Save spectrum as picture, right click mouse then select save, spectrum can
be saved as bmp, jpg, emf or png format.
Copy data in table, use “Ctrl+A” to select all data, then use “Ctrl+C” to
copy; or use mouse select data you need, right click mouse to select copy.
g. Save data
down menu to load a spectrum; by Export function operator can export the
data to Excel.
g. Report print
4.4.1. Single click on the tool bar menu, enter the quantitative
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Fig. 43
Start Measurement
Stop Measurement
Parameters setting
Establish the Curve
Save data
Print Report
Single click on the tool bar to set parameters, see Fig. 44 & Fig.45.
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Fig. 44
Fig. 45
In this window, you set measurement method, wavelength of measurement,
also you can select reference test method, calculate formula, test method
(concentration method or coefficient method, if use coefficient method,
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you need to input coefficient into coefficient settings), zero insertion (use this
function to make the curve goes through zero point).
In this window:
Method Wavelength test method
M WL Test wavelength
Reference Scan Test method of reference
Formula Calculate formula
Power Power of the formula
Conc.Unit Unit of concentration
Conc Concentration method
Coef Coefficient method
4.3.3. Establish the standard curve
At least two points are needed when establish the curve.
Separately place the reference and sample into cell holder, click the
sample on right top of the screen, change “Standard” into “Standard----
Fig. 46
According to the cueing, see Fig. 47, place the reference in light path, and
click OK.
Fig. 47
According to the cueing, see Fig. 48, place the light block in light path, and
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click OK.
Fig. 48
According to the cueing, see Fig. 49, place the sample in light path, and
click OK. Now the Abs of the sample can be obtained.
Fig. 49
Input the corresponding concentration on standard sample, and click
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Fig. 50
If the created curve is not ideal because of certain result of reference, for
example in Fig. 51, then right click mouse in the data column, then delete it.
After deleting, you can retest this standard sample and recreate this curve.
Fig. 51
4.3.4. Concentration measurement
Notice:
If selected reference test as Once, then in the following steps, it would be
unnecessary for users to test reference and block, but place the sample into
light path directly.
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Put reference and sample into cell holder, click Unknown Sample, making
Fig. 52
After that, system will inform to pull reference into light path, see Fig. 53. Pull it
in then click “OK”.
Fig. 53
Next, system will inform user to pull light block in, see Fig. 54. Pull it in, and
click “OK”.
Fig. 54
At last, system will inform user to pull sample into light path, see Fig. 55.
Fig. 55
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Pull it in and click “OK”, the measurement result will be shown, as in Fig. 56.
Fig. 56
4.3.5 Coefficient method
Parameter setting is same as Concentration method. Select Coefficient
method in test method column, and input the coefficient of known curve,
see in Fig. 57, then click “OK”, after that, system will create the curve
according to the formula that is set, see Fig. 58.
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Fig. 57
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Fig. 58
In put the unknown sample into cell position, use mouse click “unknown
If instrument is not initialized, then user click any function button, user will get
information as follow.
During the process of zeroing or testing, if click to stop, all data will
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be deleted. Click again will restart the test for the current sample.
After test finish, any change of parameter will create a new test window. If
wavelength or reference test mode is changed, system will rerun zero then
start new test; while change other parameters will not rerun zero.
When shutting off test page, system will inform you as Fig. 59 shows. Select
Yes to stay in test page, user can save tested data according to your usage;
if select No, system will quit test page.
Fig. 59
In parameter settings, same wavelength can not be set. If else, user will get
following information:
a. Save data
down menu to save spectrum; Lord to lord data; Export to save to excel.
Spectrum copy, right click mouse on spectrum, select “Copy”, then you can
c. Report print
Click on tool bar to enter DNA % Protein test mode. See Fig. 60.
Fig. 60
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In this window,
Start measurement
Stop measurement
Parameter setting
Data save
Print report
Click on tool bar to enter parameter setting window, see Fig. 61.
Fig. 61
Generally, there are two common methods for protein test, A230/A260 and
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A280/260 (users can also custom their own method). Select method, set
parameters (coefficient is already set, just set concentration, decimal,
4.4.3 Measurement
Select A230/260 method, put reference and sample into cell position, click
Fig. 62
Then pull reference into light path, see Fig.63. Then click “OK”.
Fig. 63
After that, according to tips, pull light block into light path, then click “OK”
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Fig. 64
Then system informs to pull sample in, see Fig.65, pull it in and then click
“OK”.
Fig. 65
Fig. 66
Later, instrument will inform to put reference in, pull in reference, then click
“OK”.
Fig. 67
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According to the information, see Fig.68, pull in light block, then click “OK”.
Fig. 68
At last, see Fig.69, pull sample in to complete measurement. Then click “OK”.
Fig. 69
After measurement complete, instrument will inform the operator, see Fig.70.
Click “OK”, measurement result will be shown, see as Fig.71.
Fig. 70
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Fig. 71
Notice:
When instrument is measuring, user clicks “Measure”, “Wavelength locating”
or other function buttons, system will inform as follow. Other operation shall
be done after measurement complete.
If instrument is not initialized, then user clicks any function button, user will get
information as follow.
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After a test, if a new test is needed, it is not necessary to quit test window,
enter setting window, input parameter for new test, click OK, a new test
page will created automatically.
During the process of zeroing or testing, if click to stop, all data will
be deleted. Click again will restart the test for the current sample.
After test finish, any change of parameter will create a new test window. If
wavelength or reference test mode is changed, system will rerun zero then
start new test; while change other parameters will not rerun zero.
When shutting off test page, system will inform you as follow. Select Yes to
stay in test page, user can save tested data according to your usage; if
select No, system will quit test page.
Fig. 72
a. Save data
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down menu to save spectrum; Lord to lord data; Export to save to excel.
Copy data in table, use “Ctrl+A” to select all data, then use “Ctrl+C” to
copy; or use mouse select data you need, right click mouse to select copy.
c. eport print
Click button on tool bar to edit and print report, see Fig. 73.
Fig. 73
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Appendix A Revision
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