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1. Infection of Donor:
4. Release of phage
5. Infection of recipient: The phage carrying the bacterial DNA then delivers it to the
recipient cell when it tried to infect again
6.
7. Legitimate recombination: The injected bacterial DNA may then be inserted into
recipient chromosome by homologous recombination
Fig. Complete
Transduction by
transducing phage P1
Abortive Transduction: In abortive transduction, a segment of bacterial DNA
(exogenote) is introduced into another bacterium by a bacteriophage. When the
exogenote is integrated into the recipient bacteria, this is called “complete
transduction”. But when the exogenote is not integrated into endogenote and remains
free, it is called “ abortive transduction”. When the abortive transductant divide, out of
the two-only one daughter cells contain exogenote. The other cell do not contain
exogenote. As the time progresses, the exogenote is progressively diluted out through
subsequent cell divisions. This type of transduction is known as ‘abortive
transduction”.
Normal Events Leading to Lysogeny
The steps leading to lysogeny under normal circumstances follows as:
• Circularization of the phage chr
– Cohesive ends
•Site-specific recombin
Cohesive Ends
ly
• Site-specific
recombination
Linear Double Stranded Opened C
– Phage coded
enzyme
gal
– λ phage
integrates by
Termination
• Induction
– Adverse conditions
• Role of proteases g
– recA protein
– Destruction of
repressor
• Gene expression
• Excision
Specialized Transduction- error in lysogenic cycle.
• Error in L
• Excision of the
prophage
• Replication and
release of phage
• Infection of the
During specialized transduction, only specific gene from donor bacterium is transferred
to the recipient bacterium through phages. Unlike random packaging of bacterial DNA,
this form of gene exchange results from imprecise excision of an integrated phage
(prophage).
λ phage integra
recombination (
gal B
λ bio or λ gal trans
imprec
gal B P’
λ
bio
B’ P
Due to imprecise excision, lemda bio or lemda gal transducing
phage Phages that carry a very small part of the adjacent flanking bacterial
genome, either to the left or right of the att site. These phage can
recombine into the genome via phage –mediated integration or
homologous recombination.