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Carbohydrates
Maltose
Polysaccharides Features
1. general formula:(C6H10O5)n
2. are made up of many monosaccharides joined by glycosidic bonds formed between them by
condensation reactions which involve the loss of water molecules.
Compare the storage and structural forms of starch, glycogen and cellulose and their roles in plants and animals.
Point of Comparison Starch Glycogen Cellulose
Function Plant storage polysaccharide Animal storage polysaccharide Plant structural polysaccharide
Location Stored as granules in chloroplasts Stored in liver and muscle cells Cell walls of plant cells
Monomer Made up of -glucose monomers Made up of -glucose monomers Made up of β-glucose monomers
Bond between In amylose: (1-4) glycosidic bond links (1-4) glycosidic bond links β (1-4) glycosidic bond links
monomers monomers; monomers within a branch and monomers in a molecule
In amylopectin: (1-4) glycosidic bond links (1-6) glycosidic bonds links
monomers within a branch and (1-6) monomers at branch points
glycosidic bonds links monomers at branch
points
Orientation of All glucose monomers in the chain have the All glucose monomers in the chain Adjacent glucose monomers
monomer same orientation have the same orientation rotated 180° with respect to each
other / Alternate glucose monomers
rotated 180o
Structure of each Amylose is a helical molecule while Helical and branched molecule, Long, straight chain
molecule amylopectin is a helical and branched like amylopectin, but more
molecule extensively branched
Bonds between No interchain hydrogen bonding No interchain hydrogen bonding OH groups projecting outwards in
molecules both directions allow interchain
hydrogen bonding
leads to microfibril formation
How the structures of starch and glycogen make them good How the structure of cellulose makes it a good
STORAGE molecules STRUCTURAL molecule
1. Large molecule made up of many -glucose monomers 1. Adjacent glucose units are inverted 180 with respect to each other
Hence it is a compact energy store as it can be hydrolysed to and hence form a long, straight molecule with free OH groups projecting
yield many glucose molecules that can be used as a out in both directions which can hydrogen bond with OH groups of other
respiratory substrate to obtain ATP. cellulose molecules lying parallel to it and form microfibrils.
Thus the microfibrils have high tensile strength.
2. Comprise of helices
a. can pack many subunits per unit volume 2. As a macromolecule, cellulose has few OH groups available to hydrogen
b. in which most OH groups are involved in intramolecular bond with water as most are involved in interchain hydrogen bonding.
hydrogen bonding, few OH groups available for Thus only the surface of the microfibril has OH groups that can hydrogen bond
hydrogen bonding with water. Hence they are insoluble in with water. Thus cellulose is insoluble in water and the Ψw of cells are
water and the Ψw of cells are unaffected by their presence. unaffected by its presence.
3. Amylopectin and glycogen are branched 3. The meshwork of microfibrils that form the cell wall
a. they have multiple branch ends which hydrolytic enzymes a. have a porous structure and hence the cell wall is freely permeable to
can work on. Thus more glucose molecules can be released water and solutes and allows movement of substances across the cell wall.
at the same time and more ATP can be generated by b. Are strong and rigid and distributes stress in all directions to prevent
respiration per unit time. the plant cells from bursting due to osmotic stress.
b. branching increases the accessibility of water and thus
increases the solubility of the molecule. *Note overall 4. Cellulases that hydrolyse cellulose are found in very few organisms. Thus
molecule is still insoluble in water and Ψw of cells are cellulose cannot be hydrolysed and used as a respiratory substrate and is a
unaffected by their presence. good structural molecule.
Prepared by: Mrs S Nair & Mrs Wong SH Raffles Institution (Yr 5-6) 1
The Cell & Molecules of Life (9744) Carbohydrates 2017
Test for Non- If a negative result for Benedict’s test is obtained for the Presence of non-reducing sugar indicated by:
Reducing test solution, then A blue solution remains when Benedict’s test is first carried out.
Sugars 1. boil equal volume of test solution with dilute After acid hydrolysis, Benedict’s test is carried out again
hydrochloric acid for about 1 minute to hydrolyse colour of final suspension depends on amount of sugar
disaccharide to monosaccharides present
2. cool contents of tube.
3. neutralise acidic content with sodium bicarbonate *Both observations must be made to conclude presence of non-
solution. reducing sugars.
4. carry out Benedict's test for reducing sugar.
Iodine Test for 1. Add a few drops of iodine solution to 1 cm3 of test Presence of starch is indicated by blue-black coloration.
Starch solution (or a piece of test specimen).
2. Observe any colour change.
(unbranched)
α glucose
(branched) monomer
α 1-6 glycosidic
bond at branch α 1-4 glycosidic
point bond within branch
(extensively branched)
α 1-4
α glucose
glycosidic monomer
bond within
branch
(unbranched)
β glucose
monomer
Prepared by: Mrs S Nair & Mrs Wong SH Raffles Institution (Yr 5-6) 2