PMID- 10835371

OWN - NLM
STAT- MEDLINE
DA - 20000720
DCOM- 20000720
LR - 20061115
PUBM- Print
IS - 0261-4189 (Print)
VI - 19
IP - 11
DP - 2000 Jun 1
TI - Multiple roles of Spo11 in meiotic chromosome behavior.
PG - 2739-50
AB - Spo11, a type II topoisomerase, is likely to be required universally for
initiation of meiotic recombination. However, a dichotomy exists between
budding yeast and the animals Caenorhabditis elegans and Drosophila
melanogaster with respect to additional roles of Spo11 in meiosis. In
Saccharomyces cerevisiae, Spo11 is required for homolog pairing, as well
as axial element (AE) and synaptonemal complex (SC) formation. All of
these functions are Spo11 independent in C.elegans and D.melanogaster. We
examined Spo11 function in a multicellular fungus, Coprinus cinereus. The
C.cinereus spo11-1 mutant shows high levels of homolog pairing and
occasionally forms full-length AEs, but no SC. In C.cinereus, Spo11 is
also required for maintenance of meiotic chromosome condensation and
proper spindle formation. Meiotic progression in spo11-1 is aberrant; late
in meiosis basidia undergo programmed cell death (PCD). To our knowledge,
this is the first example of meiotic PCD outside the animal kingdom.
Ionizing radiation can partially rescue spo11-1 for both AE and SC
formation and viable spore production, suggesting that the double-strand
break function of Spo11 is conserved and is required for these functions.
AD - Department of Biology, Indiana University, Bloomington, IN 47405, USA.
FAU - Celerin, M
AU - Celerin M
FAU - Merino, S T
AU - Merino ST
FAU - Stone, J E
AU - Stone JE
FAU - Menzie, A M
AU - Menzie AM
FAU - Zolan, M E
AU - Zolan ME
LA - eng
GR - GM43930/GM/NIGMS
PT - Comparative Study
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
PT - Research Support, U.S. Gov't, Non-P.H.S.
PT - Research Support, U.S. Gov't, P.H.S.
PL - ENGLAND
TA - EMBO J
JT - The EMBO journal
JID - 8208664
RN - 0 (DNA, Fungal)
RN - 0 (Fungal Proteins)
RN - 0 (Spo11 protein)
RN - EC 3.1.- (Esterases)
RN - EC 5.99.1.3 (DNA Topoisomerases, Type II)
SB - IM
MH - Amino Acid Sequence
MH - Apoptosis
MH - Chromosomes, Fungal/*physiology/radiation effects
MH - Coprinus/cytology/*enzymology/genetics/radiation effects
MH - DNA Topoisomerases, Type II/genetics/*physiology
MH - DNA, Fungal/genetics/metabolism/radiation effects
MH - Esterases/genetics/*physiology
MH - Fungal Proteins/genetics/*physiology
MH - Meiosis/*physiology
MH - Mitotic Spindle Apparatus/physiology/ultrastructure
MH - Molecular Sequence Data
MH - Prophase
MH - Sequence Alignment
MH - Sequence Deletion
MH - Sequence Homology, Amino Acid
MH - Species Specificity
MH - Synaptonemal Complex
EDAT- 2000/06/03 09:00
MHDA- 2000/07/25 11:00
AID - 10.1093/emboj/19.11.2739 [doi]
PST - ppublish
SO - EMBO J. 2000 Jun 1;19(11):2739-50.