J. Appl Biol. Chem $3(8), 248-252 (2010) LEDSHO| Chet ss BSYtS monitoringS 218+ FS HBA) Het 2214 - ojsitts Whats geese) Development of Phototactic Test Apparatus Equipped with Light Source for Monitoring Pests ‘Min-Seok Oh and Hoi-Seon Lee” Department of Bioenvironmental Chemisiry and Institue of Agricultural Science & Technology, College of Agriculture & Life Science, Chonbuk National University, Jeonju 561-756, Korea Received October 30, 2010; Accepted November 25, 2010 ‘This study presented a development of a phototactic apparatus for pest monitoring. We carried out behavioral experiment against various pest species by using the phototactic chamber. Consequently, the phototactic apparatus was confirmed suitable result of behavioral experiment. Therefore, we believed that the test apparatus help to understand the phototactic responses of various pests. Key words: behavioral experiment, light emiting diodes, phototactic apparatus, phovotactic responses AS SUS] BRS Avo) Aaa AAV) of ——_LEDALightmiting dodey'= 7B viel 38 SPE SES eA OHO vel Re oa Ee, 59, Ae Arby io] B7isisteh. leit Ada SUES A FH resol 71S ALE Marl HAT plow alee} Si} Hees LER ges Ue agzde Waa [Tamulaitis “$, 2005; Wu §, 2007], ER LEDS ©} af A FAA Slo} AS WI HIV HAS ABD, AS a] Aol Hswlel a} abel ML TBS Ye Zaely Seo] cS seh ES Azteba YEH EHvan LenterenS} NeIsay BIS Sve + Mas] aa Sol} 198% Cho, 203). VBA 3 WHA) [Kim +, 2000; Kim , 2008], ols NESE 2} 2 golf, TFL ol leh. sleHUm %, 2009}, 3} z NPD Plutella © LEDST} Ff ASSOS WE BES WHS F We WA loli, IV NSpedavera eos EAI —-B LEDIMighpover LED) AMES , 207) (Spodoptera litura), SUP H(Mamestra brassicae), Be) a Helicoverpa assulta) ‘$24 2!°| BBS] 1, 271, #2] SS 7h PRSHE do) SIA SAT Mt, alah UaHel ost spay) S7istt Wis Azole Vang, 1989), oleh Als BLS ose Fe Lead 2001), wT Weds] A WSS abs apy 9} Pe Aaa] aS ageSS walsh] et Po] 9] de} APBSI3t glow, AU] BSS] ABO’ ele ch — AFL SleHIunji , 2002; Bishop -F 2004; Bishop SF, A AVY oS we, So cee AT VE Ad Se) S 2006). Ca [2003p I sale Ah ZL] EeAsh plastic cup 2] BAlzel =Tela}) SASLCHChoi $, 2008; Paik %, 2009}. Eo] $30nm LEDS Behe Elo] ehiihFo] Es} eH al Maa sewol HE AIA] ATF Steel we} AL 71 Elo la EePSeo] AP YS|sLOV, Nombela ARID WG SUBS AISACZ AMI] Ae AMEE —[BOOGPE BA EL] EP} plastic cup [Plo] s30nma}g2] ole ADAG] Fz Qe LEDE iisle] GuReLel] opel Eseseho] Pye w —— ath. 8 Lo DMS} Ma YEIOTIFS VRBO] US *Corresponding author ©} 405 nmol} 2: 21-12 YEAHS 554nme 671 noel Phone: +82-63-270-2544; Fax: 82-63-270-2550, E-mail: hoiseon@ehonbuk.ac kr AA Fe HIS welsh ols} Jo] et SaaS 7F 42 eH eee woley) 8 Be tHe) aa doi: 10.3839/jbe-2010.043 S1Ou} ola) SAE ZAsio} Ce alSoh ayaad Bwaris Uaeele= 29 ‘Wavelenths (om) ee Forward Luminous Peak Range” Voltage Iniensty Be a0 waa 8 is Gren 50520-83034 45 Yellow S90 SSNS 22 6 Red 62s giSe3s 25 0 SIS Fl 2 71BRE Ves] SY F Ve SA Busse Aue Al olFojay) BL We Vd. aap, & ae aeel vale Wel gale Ase AOR Ae vgs AOR ca Bale} ohh, Be Bt ZAPRol Heh AA ES AS FMR alte ee LEDFEABBAE MUsHEM| 2 BA6) leh Bal BA F523 JH) Bats] US NS dala A SUS FBS FAIL, LI Bole FSS] Hest Mass Aalaisie, HV Baoe wal S- SE £08 TUS FH Ue TE B40] Be Ved GE 7 AAAS BHR Belst GO Says Le 2 Wisp] 18 Fue F9 kage Ages, 2 oo} Spe Veal Bale Hale Paso} ae}. 204 AA, Adee CEEE, 28 Det yet, = Paso] skh, 248 sede CERT aslo} ws, ANAS. LED 4S GM3SIO} SES LED SIEAKE: alc) 3 7A WRAY Bass AMaiale. B ae ABT 470 rmn(biue), 520 mm(green), S9Onmiyellow), 625 nmired) 222) LeDe G4 BGs HALA VALLES Ciel light (cou, Kora)s}2} Photron(Ansung, Korea)’HEFE} #91518 3, VISE BAA Ansing, Korea] FAIR. AEA LeDe] 248 Ale FIA ASAE data sheet’ HHS, & Table 191 Yebate}, BH ao F 7h] Yee 2a aol B77} BUHS SRT + Es Aaa B89) 1O8 AY. 71e FoISSE We ee BASS UGOR LEDS] KAY WHS SE BAe] SAS aa} 7] Hale) -B SMI) TASS gels. S AMS] AHS ULE Wom Gola A BS Haslet SH, BASS EGO koe LE LED BBE AYA A Bho] FUE SISVOL TOMI BB MES Sgsach. SLAY VBE 20 hw SAE 100 hart Repellence Ae] BAM We Sse} BIEL BLS AA w 289) tole Basis, say Adee, Fay deel dat Fed Aes J He TEMS] FATE WAzde| Bele] shh BFS seBAldel THE BUG) SPU Spodoptera exiguap MPAASTURY Spodoptera linraye] WES Baeisich. 228) LEDS vis}s|71R141, REALM332; AS ONE Co, Ltd, ‘Tokyo, Japanysk “53 AS bao] BPS Sel} TOemPH 4 Sele) WHS sass SASS Fasisteh. VA 1G INS 1 BASS BA SS HAE sol oS ake Balshatch. 7Weele zoe Hee Ett 9] Sialel wet BLO] SAS BLS oS rahe Hele 2 Oa, Beoeve} We wold Sel Male WAKE ral, ele FA SY MoV ES GE ANE wees Uealsitttig. 1. LEDESIof wat BSS] ALES Baap] gio) de FH) AVS] Bal(Fig. NE ASSL] APHGSA BE AMM Jess] Bash Fe WS aolayl #] BA] D2 Bees AVS Aes|gOuU, Oe LEDs Tad E O}AY Re UF Bal ge chest dud AISA wt AL AVGS}7IS S)StCHMutawiwa “S, 2005; Jung +, 2009], 74 ed Adee] ate Pease) salts aol) das 4, Lael HAYS) AGLE Ge Lee was TEC, Daye MAES EM, WYSE PFS) La HAR Sal Aapsisich, JWR AAO gale SEM Ge 2p LAU ES|) 2HISA}4] WES alalsiol SS AE F ESEG SE AAT a ote] CES dels) stot a AISA. Vals] AS Se Fig, 2) HVS} DSS U22 Belo) YRS WS Sopris eee oye 1 Wasi BE alae adelel FFI Weta 21 SEAL FPS Wz) YES a7) ah We. L ao] ASHHFig. dye Age) DAIS wth Fes Fg Oa AASIAOU Bao) Yeh yW adele] Habs S4sy] Hol Be BA Azle] ARE A Fig. 2 a aqeio] allio aol Pas Avista. aabspes sislch, ysoh aaled Hl. 22 PSS Ba SS TMAS Sel Eqle@e a desl sae abel dads Vast 259] Yo} et DeIAEERGYE AWE SES AR Uh FS #23871 Basie WME olgalel BASS wl Antaction + (Ditk side) ——+ No choice <—— (Light side) ——+ Fig. 1, Chusiication of phototactic response on the basis of movement250 224 + ols Fig. 2. The body of phototactic test apparatus, a: hole; b insect entrance: e: transparent pane! (the side par) ansparent panel (the upper part) ce: Cover, F Parr “ © a Fig. 3. The light sources of phototatic test apparatus. (A) LED Fight source; (B) Fluorescent light soure; (C) designation. a: LED module; bs LED circuit boar c= contol cieuit board of SS1Z Lo) He USS MS GREE Fa} OLS RCIE Nel eH Aeol E VaS AST | Mol OnE SAS BUS > Neath. AY SA Ey 7] sh F As] Uzake olesi BA Seles} Bey Asclfig We FH TSS IIL AAS WTF Apolo WEL MEL ALS B1E Woe Se O1BS Se ARIS ATER wets ate oUF £28 WAabh PIES AesiC. BOMig. 3A and BE PFSVE] AUS et Wo} oS LEDS} PRG] Beles] RAYS Bssh1 Mell 24S seus eee Y Ue) FSS asi eRe SeVS asi. aa S SITE Bel H. Bale) H 25s LEDS UAT} ch. VF a] sisi] SE Bas] HS Selb VIGO hehe (Ts.s0EW)e 71E HELE We) ABS BalOe Levys] — AAs] SB] BUS SEAS SY Bay, Table Blas st ASR AVIAT A249] LEDHMY BV — 249} De] 62Snm(Resy ShuxZ YEHES| 470nm FS ASA] IE FYse] HAE BES FAT Wa — Buys} 52nm(Greenp}> 85 lux, S9Onm(Yellow 75h 9) B8S aes DES PIR. LED See 278 — 50 it SVS BSE) vel ARNE We 2 SLED Oe Helse. BRE Woe WE Bt BRE Halon, UESKS MEA SOR 62Snm(Rode 10344, 470 nmiBlug= 8.70%, Basle] 248 size Re 485M, $90 am(Vellow)e> 32082 Basa Es zee Fle a ROE Badsiateh(Table 3). 28 JEL Fa E FU) AVIS ISS SPS exiguayh BAA AG Ae] LEDILALE eso} LEDAEMS) LEDLE — IANS. linwayel AIS WAV] Sele] BIE BGO & onoftslel CIS St BH SRE +H IS Wer ob — age] BOW TPS TIE Aah PAS exiuaye Ue} BBS Zalslol UE BES HT + ae 470 mS} 520ml) 2424 769} 87% YERLSTH, 550 SAC. BR, Belo] Wyss Sol le IVT] Ue] Samo} 62S nme 6104s} 53% UEHHo} AALS 470nms} wt SH et LEO TE SISA VE Wl 1 — SI ame] VERE FURY] waza VE AS AE > ah olse] fe SEO ME OEMS VepL + Ach Wilkes «eh RHE ose PAS] HelETS core YEMHl Lo2Re Sees) ok Bee SES Ae} 470m} 520mm BERS HAIL] oHAPAVE 500 nme} 625 4e # ES hy se aare $2pee Ads wadele= “Table 2. Mes 2st urement ofthe range of the difasion from five HPLEDs and the fluorescent light Intensity of aati (as) Waele sik Wan 2am Mem ‘Wem Wem wom oem Fon Biv) 360 8 20 187 36 vo s 520m Groen) sso 505 300 8 136 rot s 59% am (Yellow) 0 as 280 0 130 6 8 @2SnniRed) 60 32 301 89 wr 02 %6 lures (conto) 60 32 200, 2 a @ 50 ‘Table 3 luminance efficiencies among the four HPLEDs and the fuorescat light inthe est chamber Wavelet om) Uaninanee efficiency (dW i (Colo) 20h winx ‘0 ae om TOO x 70-10 nm (Ble) a5 a8 sia 5068 a5 52055 nm (Green) nao ass7 2871 aos dss 9085 rn Yellow) 1852 19.42 1905 v6 320 628210 sm (Ro) 5856 «270 tow casi 1032 38080) am (Frese) 556 606 598 5 1.00 "Relative cfciensy Gased on the mean}iluminance eficensy valve of LEDIlmioanceeficioney vale of Muorewsont “Table 4S. exigua and 5: liwra attraction rates among the four LEDs and the fluorescent contol as Attaction rite) 470mm (Blue) 520m (Green) _—_SODam(Vellow) —_—2Snm(Red) __—_ 180-800 nm (laoreseent) S exigue 76% Gi% 3% om Stitt om 41% 3% s1% “ach value ste average of 3 dtenniation after 30 min and 40 hx exposure, with 30 aul secs per replication *neaton rate (6) isthe average prcetage ofthe 30 exigna and Sura adults omucts Phe HRMS Ba a 4 Male. aia BE] APNE 470, 520, 590 B 625mm) 67, 51, 41 E Bw AION Hel URI. ested weld al BFL BREEN 470 nme HPT One W) SB AVA 590 umd} OSnmE WE HIS Bate + SkakchTable 4). Aedes 8 ate BMS Fad dpe de auehd SAstgol da Fae aes] geo] INS s+ She BAAS AAvsist, Mes) AB 7s wel of) eh Cae ls) ah, Bet SES BALIO Het BIME Th am US + Ske Bao) Neh oS Sh PE FHLS o] Se eae] dale Aus) CofE AE Se ANMaHe] Bal Aus] 7e4el ARE ATE + US A 22 Ame ae] & & SRUEY SHAPE: 710074085} aa slat 1 Fo4al 2a) gree Bishop AL, Bellis GA, MeKeizie HJ, Spohr LI, Worrall RJ, Harris, AM, and Melville T (2006) Light trapping biting midges Culicoides spp. (Diptera: Ceratopogoidae) with green iht- emiting diodes, dust J Ewomo! 45202-205, Bishop AL, Wonall R, Spohr Ll, MeKenzie , and Barcia IM (200s) Response of Culicoides spp. (Diptera: Ceratopogonidac) to lightemiting diodes. ust J Entomol 43, 184-188, ‘Cho MR. (2008) Control of major inset pest in greenhouse crops. Kor Res Soe Proteted Hort 16, 1421. (Choi BR, Leo SW, Park HM, Yoo JK, Kim SG and Baik CH (2008) Monitoring on insecticide resistance of major insect pests in plastic house. Kor J Pestic Sct 9, 380-390. (Chu CC, Jackson CG Alesandor PJ, Karu K, and Hemnebery TH (2003) Patic cup traps equipped with ight ming diodes for monitoring adult Bemisia tabaci (Homopters Aleyroidae) J Econ Entomol 96, 543-546 Hang Bl, Hs PC, Wu MS, and Tang CW (2007) Swudy of system dyaninie model and contol of a high-power LED lighting lominaie Energy 32, 2187-2198 Jung MP, Bang HS, Kim MIL, Han MS, Na YE, Kang KK, and Lee DB (2009) Response of Ussur Brown Kagi Patantcus ussurensis to ightemiting diodesLED). Kor Enron Agric 28, 468-87. Jnji H, Makoto A, Tomoki N, Toshio M, Tetsuo Y and Hiroki (2002) Studies en the consol of insect pess using illuminators ‘made fiom ultra-high uminance ightemiting diodes. Suracerisis of inet eleeuo-etinagrans (ERG) with respect {© wavelength and fequeney of pulsed light stimuli, J Jon Soe Agric Moch 64, 1682252 2a + olsial ‘Kim HY, Shin DH, ane Kim KU (2000) Effects of different UV- B levels on growth, antioxidant contents and activities of related enzymes in Cucumber (Cucwnis sativus L.) Korean J Environ Agric 19, 309-313 ‘Kim IS, Zhang C, Kang HM, and Macksy B (2008) Contol of stretching of cucumber and tomato plug seedlings using supplemental light. Hort Briar Bio 49, 287-292, Lo DM and Ma YF (1977) Stadies on the phototactic behavior of [Noctuid moths: change in reflecting power of the compound eyes and an analysis of the trapping probability. Acta Entomol Sin 20, 128-134 ‘Mutwivas UN and Tantau HY (2005) Suitability of a UV lamp for ‘rapping the greenhouse Whitey Trilewodes vaporariorum Westwood (Hom: Aleyrodidae). Agricultura Engineering Intemational: the CIGR:Ejoumal Volume Vil, Manuscript BC 05-004, Nombela G Cha CC, Henneberry TJ, and Mufiz M_ (2003) Comparison of three trap types for catching adult Bemisia tabaci whitey and its parasitoid Brermocerus munis in tomato greenhouse. LBC wprs Bulletin 26, 33-56, Paik CH, Lee GH, Kim DH, Choi MY, and Kim $s (2009) Biological contol of major pests in egaplant greenhouse. Kor J Organic Agric 41, 227-236. ‘Tamulitis G Duchovshis P, Blizikas Z, Breve K, Uliskite R, Brazityte A, Novickovas A, and Zukauskas A (2005) High- power lightemiting diode based faity for plant cultivation. J lps D: Appl Phys 38, 3182-3187. ‘Um YC, Jang YA, Lee 1G. Kim SY, Cheong SR, Oh SS, Cha SH, and Hong SC (2008) Eifects of sclectve light sources on seeing. quality of tomato and cucumber in closed nursery system. J Bioenviron conto! 18, 370-376. Van Lenteren JC and Wocts J (1988) Biological and integrated contol in greenhouses. drm Rev Entomol 33, 239-269, ‘White EG (1989) Light tapping. fequeney and data analysis ~ a reply. Z Enomol 12, 91-94 Wilkes A (1942) The influence of selection on the preferendum of 1 Chaked (Micrplecion fiscipennis Ze.) and its significance in the biological control of an insect pest Proc R Soe Lond B 134, 400-415. Wu MC, Hou CY, Jiang CM, Wang YT, Wang CY, Chen HH, and ‘Chang HM (2007) A novel approach of LED ight radiation improves the antioxidant activity of pea seedlings. Food chem 401, 1753-1758. Yang CY @001) Insect sex pheromones and their use in pest ‘management. Kor Res Soe Protected Hort 14, 30:36