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Summary
Background More than 3·5 million children aged less than 5 years die from diarrhoea and acute lower respiratory-tract Lancet 2005; 366: 225–33
infection every year. We undertook a randomised controlled trial to assess the effect of handwashing promotion with See Comment page 185
soap on the incidence of acute respiratory infection, impetigo, and diarrhoea. Division of Bacterial and
Mycotic Diseases, National
Centers for Infectious Diseases,
Methods In adjoining squatter settlements in Karachi, Pakistan, we randomly assigned 25 neighbourhoods to
Centers for Disease Control and
handwashing promotion; 11 neighbourhoods (306 households) were randomised as controls. In neighbourhoods with Prevention, Atlanta, GA 30333,
handwashing promotion, 300 households each were assigned to antibacterial soap containing 1·2% triclocarban and USA (S P Luby MD,
to plain soap. Fieldworkers visited households weekly for 1 year to encourage handwashing by residents in soap D R Feikin MD, J Painter DVM,
R M Hoekstra PhD); Health
households and to record symptoms in all households. Primary study outcomes were diarrhoea, impetigo, and acute
Oriented Preventive Education,
respiratory-tract infections (ie, the number of new episodes of illness per person-weeks at risk). Pneumonia was Karachi, Pakistan
defined according to the WHO clinical case definition. Analysis was by intention to treat. (M Agboatwalla MBBS);
Community Health Sciences,
Aga Khan University, Karachi,
Findings Children younger than 5 years in households that received plain soap and handwashing promotion had a
Pakistan (A Altaf MBBS); and
50% lower incidence of pneumonia than controls (95% CI –65% to –34%). Also compared with controls, children The Procter and Gamble
younger than 15 years in households with plain soap had a 53% lower incidence of diarrhoea (–65% to –41%) and a Company, Cincinnati, OH, USA
34% lower incidence of impetigo (–52% to –16%). Incidence of disease did not differ significantly between households (W Billhimer MS)
given plain soap compared with those given antibacterial soap. Correspondence to:
Dr Stephen P Luby
sluby@icddrb.org
Interpretation Handwashing with soap prevents the two clinical syndromes that cause the largest number of childhood
deaths globally—namely, diarrhoea and acute lower respiratory infections. Handwashing with daily bathing also
prevents impetigo.
fieldworkers provided 2–4 bars of soap per household, risk), although we specifically detail respiratory and
depending on size. Throughout the study, soap was impetigo findings in this report. Results for diarrhoea
regularly replaced during use. have been published elsewhere.16
The Procter & Gamble company (Cincinnati, OH, We defined pneumonia in children according to the
USA) manufactured all the soap for the study. The WHO clinical case definition—cough or difficulty
antibacterial soap (Safeguard Bar Soap) contained 1·2% breathing with a raised respiratory rate (60 per min in
triclocarban as an antibacterial substance. The plain individuals younger than 60 days old, 50 per min for
soap was identical to the antibacterial soap except that it those aged 60–364 days, and 40 per min for those
did not contain triclocarban. Both types were provided aged 1–5 years).19 We did not attempt to assess
as 90 g white bars without brand names or symbols. pneumonia in children aged more than 5 years. An
They looked and smelled the same and were packaged episode of impetigo was defined as a new skin eruption,
identically in generic white wrappers. Cases of 96 bars which was confirmed by the study physician. Only after
were identified by serial numbers that were matched to the diagnosed episode was recorded to have cleared by
households. Neither the fieldworkers nor the families the community health worker (on one of the weekly
knew whether soaps were antibacterial or plain. visits) could the study participant be a candidate for a
Fieldworkers provided control families with a regular new episode of impetigo.
supply of children’s books, notebooks, pens, and pencils Fieldworkers weighed children aged less than 5 years
to help with their children’s education, but they gave no at baseline and every 4 months. They used a hanging
products that would be expected to affect rates of Salter scale for children younger than 3 years and a
respiratory illness, diarrhoea, or impetigo. Fieldworkers bathroom scale for those older than 3 years. We
neither encouraged nor discouraged handwashing in calculated weight-for-age Z scores compared with the
control households, and visited both control and National Center for Health Statistics standards. Mean
intervention households with equal frequency. weight-for-age Z scores were calculated from the
Fieldworkers were extensively trained in interviewing multiple weighing and measuring sessions throughout
techniques, data recording, approaches to promote the study for every child. We classified children as
handwashing, weighing children, and clinical moderately malnourished if their mean Z score was less
assessment of ill children (including measurement of than –2·0 and –3·0 or greater, and severely mal-
respiratory rates). They were not trained to assess chest- nourished if their score was less than –3·0.
wall indrawing, because of the difficulty that trained
health-care workers have in identifying this symptom Statistical analysis
reliably.17,18 The fieldworkers identified lesions Primary hypotheses of the Karachi Soap Health Study
consistent with impetigo in children younger than were that the promotion of handwashing with
15 years old. A study physician then visited all antibacterial or plain soap would significantly reduce
households to confirm the diagnosis of impetigo and the frequency of diarrhoea and acute respiratory-tract
referred the family to locally available health-care infection, and that bathing with antibacterial soap
services. The same fieldworkers promoted would reduce the frequency of impetigo compared with
handwashing and obtained outcome data during their plain soap. Children who did not have the specific
weekly visits to intervention households. These 22 syndrome in the preceding week but developed the
fieldworkers rotated between neighbourhoods syndrome were regarded as having a new episode. Only
(including both intervention and control) throughout children who did not have the syndrome in the
the study. preceding week were defined as at risk for a new
Trained fieldworkers undertook pre-intervention episode.
baseline surveys, and identified children younger than
15 years. Children’s dates of birth were confirmed with Antibacterial soap Plain soap Control
birth certificates or immunisation records. Fieldworkers (n=300) (n=300) (n=306)
visited participating households at least once a week for Individuals per household 9·3 (4·1) 10·0 (4·8) 9·1 (4·1)
1 year (from April 15, 2002, to April 5, 2003) and asked Children less than 5 years old per household 1·7 (0·5) 1·7 (0·5) 1·6 (0·4)
the mother (or other caregiver) whether the child had Rooms in house 2·0 (0·9) 2·0 (1·0) 2·1 (0·9)
Bars of hand soap purchased in preceding 2 weeks 1·9 (0·7) 2·0 (0·7) 1·9 (0·7)
any symptoms of cough or difficulty breathing in the Households with infants 71 (24%) 64 (21%) 71 (23%)
preceding week. They also asked whether the child had Children less than 5 years old with moderate malnutrition 125 (26%) 103 (21%) 114 (24%)
any nasal congestion or coryza (runny nose). If a child Children less than 5 years old with severe malnutrition 20 (4%) 24 (5%) 19 (4%)
Literacy of mother of youngest child in household 113 (38%) 107 (36%) 98 (32%)
was symptomatic with cough or difficult breathing,
Monthly household income less than US$60 137 (46%) 131 (44%) 152 (50%)
fieldworkers counted the number of breaths the child Households with natural gas used for cooking fuel 285 (95%) 285 (95%) 298 (97%)
took in 60 s timed with a watch.
The primary outcomes for our study were diarrhoea, Data are mean (SD) or number (%).
impetigo, and acute respiratory-tract infections (ie, the Table 1: Baseline household characteristics by intervention group
number of new episodes of illness per person-weeks at
*Incidence (of new episodes per 100 person-weeks) calculated with mean of every cluster incidence weighted by person-weeks at risk for that cluster.
Diarrhoea incidence (n=197 049) Impetigo incidence (n=200 156) provide additional power for subgroup analysis, and to
Mean Difference vs Mean Difference vs control Difference vs plain- protect against inherent uncertainties in the sample-size
incidence* control (95% CI) incidence* neighbourhoods (95% CI) soap households assumptions.
(95% CI)†
Because we assigned soap promotion versus control at
Antibacterial soap 2·02 –50% (–64% to –37%) 0·61 –36% (–53% to –18%) –2% (–24% to 20%) the neighbourhood level, we also analysed primary
Plain soap 1·91 –53% (–65% to –41%) 0·62 –34% (–52% to –16%) ··
Control 4·06 ·· 0·94 ·· ··
health outcomes at this level. Specifically, within every
neighbourhood, we identified the total number of new
*Mean incidence (episodes per 100 person-weeks) calculated with mean of neighbourhood rates weighted by person-weeks at episodes of illness (in person-weeks in the subgroups of
risk from every neighbourhood. †Accounts for clustering by household.
interest) and divided it by the total number of person-
Table 3: Primary diarrhoea and impetigo outcomes in children younger than 15 years by intervention weeks at risk for children in that neighbourhood within
group the subgroup of interest. Incidence rates by intervention
assignment (the mean of appropriate neighbourhood
incidence rates) were weighted by the number of person-
We calculated a sample size of 220 households per weeks at risk from all neighbourhoods that contributed
intervention group, assuming the same rates of impetigo to the mean. We calculated rate ratios by dividing the
as those recorded in the previous Karachi study13 (ie, 2·07 mean incidence from intervention neighbourhoods by
episodes of impetigo per 100 person-weeks in the control that from control neighbourhoods. Because we
group, 1·58 episodes per 100 person-weeks in the plain- compared antibacterial soap with plain soap at the
soap group, and 1·10 episodes per 100 person-weeks in household level, we also analysed the difference in
the antibacterial-soap group). We assumed a mean of 3·8 impetigo incidence between antibacterial and plain soap
children per household, 95% follow-up, 10% dropout, at this level.
and a doubling of sample size to offset the effect of We calculated 95% CIs of rate ratios using Taylor
clustering by neighbourhood and repeated measures. We series approximations to obtain standard errors. The
increased the sample size to 300 households per group to difference (%) in outcome between intervention and
10
Control
Antibacterial soap
Plain soap
8
Episodes per 100 person-weeks
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control was reported (ie, rate ratio minus one). We reason for missing follow-up information was that the
regarded differences (%) between groups as significant family had temporarily left town to visit relatives.
if 95% CIs excluded 0%. At baseline, households in the three intervention
After the 1 year of planned data collection was groups were similar (table 1). Households had a mean
completed, Procter & Gamble provided the list of 9·5 residents (SD 4·4) and purchased about one bar
identifying which cases of soap were antibacterial or of hand soap per week. During the study, households
plain for analysis. Disease experiences of every child, assigned to handwashing promotion received a mean
household, and neighbourhood was tracked and of 3·3 bars of study soap per week; thus, every
analysed with the group they were originally assigned household resident used an average of 4·4 g of soap per
to (intention-to-treat analysis). day.
Neighbourhoods that received soap and en-
Role of the funding source couragement to wash their hands and bathe had
One author (WB) was an employee of the study sponsor substantially less respiratory disease, impetigo, and
(Procter & Gamble). He reviewed and provided diarrhoea than controls (tables 2 and 3; diarrhoea data
feedback on the study protocol, analysis, and also reported elsewhere).16 Children younger than
manuscript. The corresponding author had full access 15 years who lived in households that received plain
to all the data in the study and had final responsibility soap in neighbourhoods where handwashing was
for the decision to submit for publication. The Centers promoted, had more than a 50% lower incidence of
for Disease Control and Prevention retained the right cough or difficult breathing and of congestion or coryza
to decide what to submit for publication and to publish than did children in control neighbourhoods. Children
results without approval from Procter and Gamble. aged less than 5 years who received plain soap in
neighbourhoods where handwashing was promoted had
Results a 50% lower incidence of pneumonia than did children
The 36 neighbourhoods in the study had a median of in control neighbourhoods. Incidence of acute
26 participating households per neighbourhood respiratory illness in households receiving antibacterial
(IQR 21–30). In the 25 neighbourhoods randomly soap was much the same as that in households receiving
assigned to handwashing promotion, antibacterial soap plain soap (table 2).
was randomly allocated to 300 households and plain Incidence of impetigo was 36% lower in children in
soap to 300 households. 11 neighbourhoods (306 house- households that received antibacterial soap and
holds) were randomly assigned to undertake standard encouragement to bathe every day than in those in
habits and practices (control). During 51 weeks of control neighbourhoods but only 2% lower than in
follow-up, information was obtained on 210 133 person- children in plain-soap households (table 3). Mean
weeks, representing 89% of the study populations’ duration of impetigo episodes was 18 days (SD 14) for
experience. Follow-up was similar in the three children in antibacterial-soap households, 18 days (13)
intervention groups (figure 1). The most common for those in plain-soap households, and 22 days (15) for
20
Control
Antibacterial soap
Plain soap
15
Episodes per 100 person-weeks
10
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Figure 3: Incidence of cough and difficult breathing in children younger than 15 years
40
35 Control
Antibacterial soap
Plain soap
30
Episodes per 100 person-weeks
25
20
15
10
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those in control households. Weekly prevalence of person-weeks). There was less difference in pneumonia
impetigo among children in households that received incidence in handwashing-promotion versus control
antibacterial soap and encouragement to bathe daily was neighbourhoods in children younger than 1 year than
47% lower (–61% to –34%) than those in control the difference seen in children up to the age of 5 years
neighbourhoods, but only 4% lower than children living (table 4). Pneumonia incidence in children who received
in plain-soap households (–28% to 19%). plain soap was nearly 30% lower in those younger than
For the first 6 months of the trial, incidence of 1 year, more than 50% lower in those aged 1–2 years,
pneumonia in children younger than 5 years who lived and just over 50% lower in those aged 2–5 years,
in neighbourhoods receiving handwashing promotion compared with controls. Age-specific incidence of
with soap was only 6% lower (–43% to 32%) than in pneumonia was closely similar between households
children in control neighbourhoods (figure 2). However, receiving antibacterial versus plain soap (table 4). When
in the second 6 months, when pneumonia was much data for households that received both types of soap were
more frequent in the control group, incidence of combined, children younger than 1 year had a 27%
pneumonia was 66% lower (–79% to –53%; figure 2). A lower incidence of pneumonia (–77% to 24%), a 36%
similar pattern of illness rates was noted in the lower incidence of cough and difficulty breathing (–59%
incidence of cough or difficulty breathing and to –12%), and a 39% lower incidence of congestion and
congestion or coryza in all ages of participants (figures 3 coryza (–56% to –22%) than controls.
and 4). Moderately malnourished children had the same risk
Compared with children aged 2–5 years, children aged of pneumonia as non-malnourished children (2·9 vs
less than 1 year had a 1·4 times greater incidence of 2·8 episodes per 100 person-weeks); but severely mal-
pneumonia (2·8 vs 2·1 episodes per 100 person-weeks) nourished children had 1·4 times the incidence of
and children aged 1–2 years had a 3·5 times higher pneumonia of non-malnourished children (4·2 vs
incidence of pneumonia (7·2 vs 2·1 episodes per 100 2·9 episodes per 100 person-weeks, p=0·003).
Individuals aged less than 1 year (n=7679) Individuals aged 1–2 years (n=9238) Individuals aged 2–5 years (n=41 675)
Pneumonia Difference vs Pneumonia Difference vs Pneumonia Difference vs
incidence* control (95% CI) incidence* control (95% CI) incidence* control (95% CI)
Antibacterial soap 2·65 –24% (–80% to 32%) 5·93 –45% (–69% to –21%) 1·55 –51% (–70% to –33%)
Plain soap 2·46 –29% (–78% to 20%) 5·04 –54% (–74% to –33%) 1·56 –51% (–68% to –34%)
Control 3·48 ·· 10·86 ·· 3·17 ··
*Incidence (episodes per 100 person-weeks) calculated with mean incidence of every cluster weighted by person-weeks at risk for that cluster.
Nutrition status No malnutrition (n=38 830) Moderate malnutrition (n=13 604) Severe malnutrition (n=3591)
(person-weeks at risk)*
Pneumonia Difference Pneumonia Difference Pneumonia Difference
incidence† vs control (95% CI) incidence† vs control (95% CI) incidence† vs control (95% CI)
Antibacterial soap 2·42 –40% (–61% to –20%) 1·83 –63% (–80% to –46%) 4·18 –27% (–60% to 6%)
Plain soap 2·12 –48% (–65% to –31%) 2·24 –54% (–75% to –34%) 3·08 –46% (–74% to –18%)
Control 4·07 ·· 4·91 ·· 5·73 ··
*Total number of person-weeks at risk is lower than total in table 3, because of missing anthropometry data for 96 children. †Incidence (episodes per 100 person-weeks) calculated with
mean incidence of every cluster weighted by person-weeks at risk for that cluster.
Table 5: Mean pneumonia incidence in children younger than 5 years by nutrition status and intervention group
Handwashing with soap was effective against Soap and the mechanical activity of rubbing hands
pneumonia in well-nourished and malnourished together enhances the capacity of water to solubilise and
children (table 5). Compared with controls, pneumonia to remove dirt and pathogens from hands.27,28 This
incidence in households that received plain soap was physical removal of pathogens reduces the efficiency of
roughly halved in children irrespective to who was non- not only pathogen transmission from the hands of an
malnourished, moderately malnourished, and severely infected person to those of an uninfected person, but also
malnourished. Households using antibacterial soap had the transmission of infectious organisms from the hands
rates of pneumonia comparable to rates for plain soap, of an uninfected person to the respiratory tract.
although the fall in rate was not significant in children Triclocarban is effective in vitro against some species of
with severe malnutrition living in households receiving Streptococcus, but does not have substantial activity
antibacterial soap (table 5). against gram-negative bacteria or viruses.29,30 The closely
similar effectiveness of antibacterial and plain soap
Discussion suggests that the physical removal of pathogens from
In squatter settlements of Karachi, where diarrhoea and hands and skin with soap and water, rather than the
acute respiratory infections are leading causes of death, specific antibacterial activity of triclocarban, is the key
washing hands with soap reduced both syndromes by factor in the prevention of diarrhoea, impetigo, and
half. Incidence of impetigo also fell by almost a third in respiratory infections.
households encouraged to regularly wash hands and Siblings and other children transmit most respiratory
bathe daily. Handwashing was effective in reducing pathogens to young children.31–33 Handwashing that
disease incidence in malnourished children, although interrupts transmission of respiratory pathogens in older
the reductions in the youngest children were not siblings and other neighbourhood children not only
significant. However, we did not detect any difference in reduces their own rate of respiratory illness, but also
disease incidence between the use of antibacterial and shrinks the pool of people who are transmitting and thus
plain soap. restricts exposure in vulnerable children.
The reduction in incidence of disease was almost In our study, respiratory infections did not decrease
certainly due to handwashing with soap only, because immediately after the introduction of handwashing
interventions were randomly assigned to neigh- promotion, but fell substantially after several months.
bourhoods, and the participating households had similar The changing of handwashing habits takes time34 and
characteristics known to affect the incidence of diarrhoea could need several months for the habit to be taken up by
and respiratory disease. Indeed, incidence of respiratory enough family members to interrupt respiratory
disease did not vary between neighbourhoods for the pathogen transmission. Furthermore, respiratory
first several months of the study. The trebling of soap disease is seasonal in Pakistan; typically more than twice
consumption in handwashing promotion households as many cases take place from October to March
from baseline suggests that handwashing was more compared with April to September.35,36 Children living in
regularly practised. Moreover, although this study handwashing promotion neighbourhoods avoided the
specifically shows a fall in pneumonia frequency from typical winter peak in respiratory disease, suggesting that
handwashing, the finding is consistent with data in other the transmission of organisms responsible for the winter
settings that have suggested a reduction in respiratory peak is efficiently interrupted by regular handwashing.
disease from handwashing with soap.8–12 Most childhood respiratory-tract infections are mild
These findings are biologically plausible. At least some and do not lead to childhood death. It is less clear from
of the viruses that usually infect the respiratory tract are our data if many severe, life-threatening, lower
readily transmitted from person to person by hand respiratory-tract infections were prevented. Fieldworkers
contact.20,21 In developing countries, viruses commonly used the WHO case definition (integrated management
cause pneumonia.22,23 Additionally, several viruses that of childhood illness [IMCI]) for pneumonia to identify
infect the human respiratory tract predispose children to children with lower respiratory-tract infections. They
bacterial pneumonia.24–26 were not trained to recognise lower chest-wall indrawing
or other signs of severe pneumonia that are highly placebo-controlled trial that was masked and sufficiently
associated with mortality. In clinics, pneumonia is powered, suggests that the addition of triclocarban to
typically over-diagnosed with the IMCI case definition. soap does not prevent impetigo in this setting and that
For example, of 965 Kenyan children who were the 23% difference in the earlier study was due to the
classified as meeting the IMCI case definition for chance selection of small, non-representative study
pneumonia, 38% were judged not to have the disease groups. Indeed, antibacterial soap did not provide a
based on chest radiography and physician diagnosis.37 health advantage over plain soap for any of the health
We would expect the IMCI case definition to identify an outcomes in our study. Soap companies should
increased proportion of false-positive diagnoses of consider whether the funds and marketing efforts spent
pneumonia in communities, because risk of disease is in the addition of antibacterial compounds to soap
lower in these individuals than in clinic attendees. would be better deployed to improve the lathering and
However, the study results suggest that handwashing sensory experience of handwashing to make the action
with soap prevents at least some cases of severe more pleasant and to actively promote regular hand-
pneumonia. First, some mild upper respiratory-tract washing.
infections in children and in their siblings predispose to Study personnel and participants were not masked to
severe lower respiratory-tract infection.24–26,38 Thus, the intervention. Thus, study participants in the
prevention of the mild, upper respiratory-tract handwashing promotion groups, grateful for the soap,
infections can also stop severe pneumonia. Second, in could have kept reported episodes of illness in the
the study, handwashing with soap was effective against household to a minimum, as could have fieldworkers
pneumonia in some children at high risk for death from recorded fewer episodes because of a desire, conscious
pneumonia, such as those with severe malnutrition.39–41 or not, to meet the expectation of the study sponsors.
Incidence of pneumonia was roughly halved in these However, fieldworkers were formally trained, and the
children if they lived in a household in which importance of accurate recording of reported symptoms
handwashing with plain soap was promoted. Of the episodes was stressed. Supervisors, who regularly made
youngest children—ie, those aged less than 1 year who unannounced households visits, identified symptoms
make up most deaths from acute respiratory infection— that were different to those reported by fieldworkers in
handwashing with soap had less of an effect. The less than 1% of visits.16
reduction in pneumonia (close to 30%) was not Our data show that regular handwashing with soap is
significant in these young children from households very effective in preventing diarrhoea16 and respiratory
with handwashing promotion. This non-significance disease, two of the leading causes of global childhood
probably indicated restricted statistical power. Indeed, death. Handwashing with daily bathing also prevents
for the other respiratory outcomes that occur more impetigo. Provision of free soap, frequent community
frequently than pneumonia, the reduction in incidence meetings, and weekly household handwashing
in infants was significant. Infants were not washing promotion visits to all impoverished households world-
their own hands, nor were families encouraged to wash wide is prohibitively expensive. Thus, the challenge for
infants’ hands. Because the removal of pathogens from the public-health community is to identify cost-effective
older people is the only route of transmission to infants techniques for handwashing promotion that can reach
that handwashing would interrupt, a more moderate the hundreds of millions of households at risk.
reduction in disease in infants than in older children is Contributors
expected. Ultimately, the proportion of severe res- S Luby developed the idea for the study, drafted the study protocol,
piratory illness that is prevented with handwashing analysed the data and drafted the manuscript. M Agboatwalla assisted in
development of the protocol, designed the handwashing promotion
needs to be established. intervention, supervised field implementation of the protocol, assisted in
These data, consistent with those of other interpretation of the data, and helped revise the manuscript. D Feikin
investigations,13,42 suggest that daily bathing with soap assisted in developing the assessment for acute respiratory-tract
lowered the incidence of childhood impetigo. The study infections, and provided published work from previous studies and
critical input to place these findings within the broader context of
showed no added benefit of including triclocarban in respiratory diseases. J Painter designed the data management strategy,
soap. This finding differs from previous data13 from developed the database for data entry and verification, assisted in
Karachi suggesting that children in households with analysis, interpretation, and revision of the manuscript. A Altaf assisted
tricloclarban-containing soap had a 23% lower in developing the protocol addressing human subject issues, assisted in
project administration, and reviewed the manuscript. W Billhimer
incidence of impetigo compared with those in critically reviewed the study protocol, analysis, and manuscript.
households receiving placebo soap. However, the R M Hoekstra helped develop the analytic strategy, assisted in analysing
difference in the earlier study was not significant the data, and had the final say on appropriateness of analytic methods.
(p=0·28). Our present study enrolled nearly four times Conflict of interest statement
as many households, and was intentionally designed S Luby was supported by the grant from the Procter & Gamble company
with sufficient power to detect a 23% difference that funded this study. W Billhimer is an employee of the Procter &
Gamble company. The other authors declare that they have no conflict of
between triclocaraban-containing and placebo soap interest.
types. The absence of an effect in a randomised,