Synopsis of thesis (M.

Pharm)
Thesis title
Phytochemical & Pharmacological Evaluation of Bangladeshi edible aquatic plants of
“Linderniaceae” family

Background

Lindernia comprises about 80-100 species, and occurs throughout the tropical and warm
regions of the world. They have been traditionally used in the treatment of many diseases for
a long time throughout the world. The aerial parts are widely used in poultices for boils, sores
and itches. Lindernia is also often used to treat dysentery and other intestinal problems. The
juice of the aerial parts of Lindernia crustacea, mixed with turmeric (Curcuma longa L.) and
heated with a little water, is applied to infected fingernails. In Peninsular Malaysia, a
decoction of the leaves is given after childbirth. But a few researches have been carried out
on their pharmacological activity.

Literature review

 Chemical components of Lindernia ciliata (Colsm) Pennell.

Lan S; Zhongguo Zhong Yao Za Zhi. 1996 Jan; 21(1):38-9, 64.

 In vitro antioxidant properties and total phenolic contents of wetland medicinal

plants in Taiwan
Yu-Ling, Shyh-Shyun, Jeng-Shyan, Yaw-Huei, Yuan-Shiun, and Guan-Jhong;
Botanical Studies (2012) 53: 55-66

 Antimicrobial, Antioxidant, and Anti-Inflammatory Activities of Essential Oils

from Five Selected Herbs

Mei-Lin TSAI Chih-Chien LIN

Mei-Lin TSAI, Chih-Chien LIN, Wei-Chao LIN,
Wei-Chao LIN
Chao-Hsun YANG;
Bioscience, Biotechnology and Biochemistry; Vol. 75 (2011) No. 10 P 1977-1983

 Observations on the Vascular Wall Flora of Banaras Hindu University Campus,

India
A Singh - Bulletin of Environment, Pharmacology and Life …, 2011 - bepls.com

 Overview of Pharmaceutical Research on Lindernia All

G Jiang - Journal of Anhui Agricultural Sciences, 2011 - en.cnki.com.cn

 Antibacterial activity of some selected folklore medicinal plants from west Nepal
M.P. Panthi and R.P.Chaudhary

1
Aims and Objectives
I want to perform the following tests-

Experiment type

 Antioxidant activity test

Methods:
 TRAP assay
 DPPH assay
 DMPD assay
 FRAP assay
 ORAC assay etc.

 Antidiarrhoeal activity test

Methods:
 castor oil induced diarrhoea in mice
 Analgesic activity test
Methods:
 Acetic acid induced writhing method
 Radiant heat method
 Formaldehyde induced writhing method
 Hot plate method.
 Antimicrobial activity test
Methods:
 Diffusion Method
 Dilution Method
 Bioautography Method
 Cytotoxic activity test
 Anthelmintic activity test
 Oral Anti-hypoglycaemic activity test

Materials
Apparatus

 Filter paper
 Cotton
 A glass jar
 Aluminium foil
 Grinding machine

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  Test tubes
 Feeding needle & Insulin Syringe
 Volumetric flask
 Beaker
 Young Swiss-albino mice
 Electronic balance
 distilled water
 TLC plates
 Filter paper
 Spray gun
 Pipette
 Spatula
 Pencil
 Glass rod
 Glass watch
 Spirit lamp
 Electronic balance
 Magnetic stirrer
 Volumetric flask
 Capillary tube as spotter
 Measuring cylinder
 UV detector
 Hot plate.
Reagents

 Mayer’s Reagent
 Dragendroff’s Reagent
 Fehling’s Solution A
 Fehling’s Solution B
 Benedicts Reagent
 Molish Reagent
 Libermann-Burchard Reagent
 Chloroform (CHCl3)
 Methanol (CH3OH)
 N-hexane
 Acetone

3
  Distilled Water
 DPPH (0.02 % w/v) solution in methanol
 Plants extract solution in methanol
 Ascorbic Acid (Vitamin C) as standard.

Possible outcomes
These plants are still being used in different part of the world so effectively and their use is
also supported by some researchers. So I expect most positive outcomes from my
experiments to be done.

Conclusion
Lindernia includes a large number of species that are found to be effective in many diseases.
Some of their efficacy has been scientifically proved and some are yet to be proved. So I can
expect a positive result from this experiment and if I get so, I think that should be a crucial
one for medical world.

SUBMITTED TO,

Professor Dr. Md. Golam Hossain

Pharmacy Discipline
SUBMITTED BY,
Khulna University, Khulna
Md. Ali Imam Razu

Roll no. : MS-131127

Pharmacy Discipline

Khulna University, Khulna