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If you are having problems understanding how these answers were arrived at
you should attend the data handling workshop in Week 6.
(1) Calculate the final concentrations if;

(a) 5ml of 1M NaCl was made up to 80ml with water. (0.0625M HCL)
(b) 1μl of 4M HCL was diluted to 1dm3 with water. (0.004mM or 4.0x10-6M HCL)
(c) 50μl of a 30mM glucose solution was added to 0.95ml water. (1.5mM glucose)

## (2) Explain how you would dilute the following;

(a) 50% (v/v) aqueous ethanol to 3% (v/v) aqueous ethanol.
(6 parts 50% ethanol + 94 parts water)
(b) 20 mg ml to 1μg cm . (0.005 parts 20 mg ml-1 plus 99.995 part water)
-1 -3

(3) If you dissolved 0.7g of NaOH (MW=40) in water to give a final volume of
160ml;

## (a) What would the % concentration of NaOH be? (0.437% NaOH)

(b) What would the concentration of NaOH in mM be? (109.25mM NaOH)

(4) You have been asked to make up 0.3 litres of a 60 mM solution of glucose
(MW=180). What weight of glucose (in grams) would you require? (3.24g glucose)

(5) You have added 20 μl of a stock solution of glucose to a cuvette to give a final
volume of 1 ml. The concentration of glucose in the cuvette was found to be 2.5μmol
ml-1. What was the concentration of glucose (in mM) in the stock solution?
(125mM glucose)

6) 5 grams of sodium hydroxide (NaOH M.W. = 40) were dissolved in enough water
to give 200 ml of solution. Calculate the normality of the solution.
0.625 N (remember HCL  H+ + CL-, i.e, Molarity = Normality)

7) How many grams of calcium hydroxide, (Ca(OH)2 M.W = 74) are required to
prepare 150 ml of 0.15 N Ca(OH)2 solution?
(here Normality = 2 X Molarity. So need to make up a 0.075 M solution) i.e. 0.835g