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FEATURE

Environmental and public health


risks associated with chemical
waste from research and
educational laboratories in
Dar es Salaam, Tanzania
This study presents a preliminary assessment of waste management practices in eleven laboratories of six
educational and research institutions in Dar es Salaam, Tanzania. Categorical questionnaires were admin-
istered to 52 laboratory personnel to assess their awareness of health and environmental risks of chemical
waste and the waste management practices. A chemical analysis was carried out to determine the nature,
amount and composition of waste so as to assess the associated risk of environmental contamination and
human exposure. The study found that liquid waste generated by the surveyed laboratories was mostly acidic
in nature (pH = 1.2–3.4) and contaminated by elements such as Cu (0.02–3.44 mg/L), Cr (0.01–0.19 mg/L)
and Pb (0.12–2.33 mg/L), in some cases above the safety limits. Generally, improper waste handling
procedures were observed in terms of unlabeled waste containers (72.3%), lack of proper collection plan
(75.0%), unsuitable means of disposal that included direct discharge into the drainage system (53.8%) and
open dumping (42.4%) as well as lack of proper records of the waste quantities (55.8%). Regarding personnel
awareness, >90% of the contacted personnel indicated that they were knowledgeable about pollution
prevention concepts and impacts associated with chemical waste, while 25% had formal training on
chemical waste management. The findings of this study raise awareness of the risks associated with chemical
waste from this potential source and call for proper management of chemical waste, particularly to
laboratory scientists and policy makers. It may also motivate a new generation of environmentally conscious
scholars.

By Charles Kihampa, associated with chemical waste from different categories of people such as
Harieth Hellar-Kihampa research and educational activities in academic staff, technicians, research-
Tanzania are not well addressed. For ers, administrative staff, students
example, waste emission from this (bachelor, master and PhD) and some-
INTRODUCTION potential source is currently not times visitors, making waste manage-
addressed in the National Environ- ment of particular importance for the
The potential risks of environmental mental Policy.1 Furthermore, studies protection of human health.6,7 As a
contamination and human exposure on hazardous waste management in general case, the environmental and
the country have focused only on such public health concerns of poor man-
sources as industrial activities, agricul- agement of chemical waste are widely
Charles Kihampa is affiliated with the ture, mining and medical facilities.2–5 discussed. These include possible
School of Environmental Science and This indicates that there is a lack of leaching to the environment and sub-
Technology, Ardhi University, P.O. knowledge on the existence of a poten- sequent contamination of surface and
Box 35176, Dar es Salaam, Tanzania tial threat from educational and re- ground water, especially in the case of
(Tel.: 713 269069; fax: 22 2775391; search institutions’ laboratories as a liquid waste,8 effects to biodiversity9
e-mail: kihampa@yahoo.com). source of chemical waste. and possible occupational exposure
Educational and research laborato- leading to health effects.10 A wide
Harieth Hellar-Kihampa is affiliated ries conduct experiments for the range of wastes may arise from a chem-
with the Institute of Continuing Edu- training and research purposes in ical laboratory in the course of con-
cation, the Open University of Tanza- which chemicals of different nature ducting experiments.11 Examples
nia, P.O. Box 23409, Dar es Salaam, and properties are used. In most cases, include liquids, such as aqueous solu-
Tanzania. these laboratories are composed of tions, oils and solvents; sludges, which

1871-5532 ß Division of Chemical Health and Safety of the American Chemical Society 19
http://dx.doi.org/10.1016/j.jchas.2015.01.015 Elsevier Inc. All rights reserved.
can be both aqueous and non-aqueous; including some possible hazardous 139.3 km2. It is the largest city in the
solid materials such as expired chemi- materials to the environment. country and the most important for
cal reagents, used packaging materials Review of literature has shown that both business and government. The
and remains of samples. Gaseous emis- wastes from educational and research city has a population of about 4.4 mil-
sions, such as those from laboratory laboratories in Tanzania have not yet lion (2012 Census) representing 10%
fume chambers may be described as received sufficient attention. There is of the total Tanzania Mainland popu-
waste on their own wide definition.12 no documented study that investigated lation. It consists of three local govern-
Currently there is a wide awareness the production rate, the handling prac- ment areas or administrative districts
of the need for proper control of waste tices and the disposal procedures of namely Kinondoni, Ilala, and Temeke.
from educational and research institu- waste from research laboratories in Dar es Salaam City was selected for
tions. Various researchers have em- the country. The existing techniques this research because it contains a
phasized the importance of proper and practices of chemical waste man- significant number of academic and
waste management practices in labo- agement, awareness levels and compe- research institutions compared to the
ratories.6,13 Institutions in various tency of laboratory practitioners in other parts of the country.
countries have developed their own handling waste from this potential This study was designed to prelimi-
guidelines for proper management source are other important issues that nary assess selected laboratories in Dar
practices for all aspects of the han- are yet to be investigated. This study es Salaam City which include three
dling, storage, and disposal of chemical was therefore designed to preliminary academic institutions, namely Univer-
wastes.14,15 Tanzania as one of the assess the current chemical waste gen- sity of Dar es Salaam (UDSM), Ardhi
developing countries strives to achieve eration and the waste handling prac- University (ARU) and Muhimbili Uni-
good waste management system. How- tices in the six institutions in Dar es versity of Health and Allied Sciences
ever, it is yet to establish standard pol- Salaam City by determining levels of (MUHAS); and three research institu-
icies and guidelines for chemical waste awareness of the practitioners, the tions, namely Tanzania Bureau of
management for its educational and quantities of wastes generated as well Standards (TBS), Tanzania Govern-
research institutions.1 Some institu- as their physical and chemical charac- ment Chemist Laboratory Agency
tions in the country have formulated teristics. The results are expected to (GCLA) and Tanzania Food and
their own operation procedures for serve as a baseline for more detailed Drugs Authority (TFDA).
handling chemical waste while others investigation in the future and also Table 1 gives a summary of labora-
are yet to do so. used as a basis for making appropriate tories of the six institutions that were
In Tanzania, the number of research recommendations. involved in this survey. Some of the
and educational institutions has kept institutions like UDSM and TBS had
increasing over the years. For example, more than one laboratory specialized
whilst up to the mid 1980s there were METHODS in research and academic activities
only two universities and a handful of that use a variety of chemicals for
other specialist higher education col- Study Area and Design experiments, sample storage and re-
leges, by 2005 there were a total 30 Dar es Salaam city is located at 68480 search. Some of the laboratories like
universities, the majority of these being South, 398170 East along the Indian the GCLA, TBS and TFDA function as
private. In addition, there were 15 ad- Ocean coast and covers an area of service laboratories providing services
ditional public Institutions of Higher
Education including six professional
institutes and two institutes of technol-
Table 1. Summary of the Laboratories Involved in this Study.
ogy.16 A good percentage of these insti-
tutions are located in Dar es Salaam Institution Investigated laboratory Code
city, the economic and administrative UDSM Inorganic Chemistry, Department of Chemistry UDSM1
center of Tanzania. With the advance- Physical Chemistry, Department of Chemistry UDSM2
ments of science, technology and inno- Organic Chemistry, Department of Chemistry UDSM3
vations as well as expansions of Chemistry Laboratory, College of Engineering UDSM4
enrolments in different fields of spe- and Technology
cialization, the usage of chemicals in
scientific laboratories of these institu- TBS Chemistry Laboratory 1 TBS1
tions are likely to increase. Laborato- Chemistry Laboratory 2 TBS2
ries in these institutions range from Chemistry Laboratory 3 TBS3
those of chemistry, biology, chemical ARU Environmental Engineering Laboratory ARU1
processing engineering, biochemistry,
botany, zoology and aquatic science. MUHAS Chemistry Laboratory, Institute of Traditional MUHAS1
All of them use different types of che- Medicine
micals and reagents on a daily basis. GCLA Chemical Management Division GCLA1
This indicates potential discharge of
TFDA Chemistry TFDA1
significant amounts of chemical waste,

20 Journal of Chemical Health & Safety, November/December 2015


to various clients. Data collection for waste was not segregated thus includ- well as pollution prevention concepts,
this study therefore involved eleven ing both liquid and solid, a third cate- accounting to percentage response of
laboratories. gory of waste termed ‘mixed’ was more than 90% (Table 2). Notable
indicated. Unit generation rates in however is a significantly low level of
Data Collection liters/month for liquid waste and awareness on specific policies, guide-
Both qualitative and quantitative data kg/month for solid waste and mixed lines and regulations on efficient labo-
were gathered through questionnaires, waste was computed by summing up ratory waste management practices
informal discussions, observation and the daily weights to obtain the monthly (3.8%). This is to be expected since
chemical analysis. Similar sets of ques- rates and then computing their respec- no such policies, guidelines or regula-
tionnaires were administered to labo- tive mean values. tions are enforced among educational
ratory technicians, chemical engineers The data from the survey were ana- and research institutions in Tanzania.
and heads of departments, regardless lyzed by simple descriptive statistics Majority of the workers (88.5%)
of their job titles and levels of educa- (sums, ranges, means and percentages) acknowledged no objection from the
tion. The questionnaires included using the Statistical Package for Social surrounding community on possible
some open and close questions that Sciences (SPSS) version 17.0 comput- negative impacts of the existing waste
aimed to assess the respondents’ level er software package. management practices employed by
of formal training on chemical waste their respective laboratories, particu-
management, their knowledge of waste Characterization of Waste larly on the disposal methods. This is
management system in terms of collec- Samples of liquid waste generated probably due to the low level of aware-
tion, storage, transportation, treatment from the investigated laboratories were ness on the part of the public as well.
and disposal based on the recommen- collected in metal cans and taken to Another important observation was
dations from the WHO (1999) guide- the Environmental Science Laborato- a low percentage of the contacted
line. The questionnaires included the ry at the Ardhi University for analysis. workers who had undergone formal
following components: The pH values of the liquid waste were training on chemical waste manage-
measured by using a calibrated pH ment (25%). This is of concern since
1. Waste collection practices meter and their heavy metal contents laboratory workers have an important
2. Waste segregation targeting a number of elements were role to play in laboratory waste man-
3. Storage and handling of liquid measured by AAS using standardized agement process especially in minimi-
wastes protocols. zation of contamination. It is therefore
4. Storage and handling of solid important to equip them with adequate
waste knowledge, skills and technology.
5. Covering and labeling of waste RESULTS AND DISCUSSION
containers Nature, Amount and Composition of
6. Use of personal protective gears Awareness of Laboratory Workers on Chemical Waste Generated
7. Availability of temporary waste Chemical Waste Management The survey results indicated three cat-
storage facility The results of awareness of laboratory egories of chemical waste generated in
8. Availability of transportation of workers of the investigated institutions the laboratories; liquid, solid and
waste to disposal site on management of chemical waste are mixed waste. Gaseous waste which
9. Maximum time period for tempo- summarized in Table 2. can be regarded as another category
rary storage Generally, the contacted individuals of chemical waste was not within the
10. Types of disposal method used showed positive response in terms of scope of this study. Generally, two cat-
11. Pre-treatment of waste prior to their awareness of health and environ- egories of chemical waste were found
disposal mental impacts of chemical waste as to be the most common (1) obsolete
12. Hazard communication system
Table 2. Survey of Laboratory Workers Knowledge on Waste Handling Practices.

Informal discussions and observa- Variable % of


tion that also included photo shots respondents
aimed at documenting and illustrating (n = 52)
the actual waste handling practices in Yes No
the respective laboratories to supple-
ment the questionnaire responses. Health impacts of poor chemical waste management 98.1 1.9
Quantitative analysis of the liquid Environmental impacts of poor chemical waste management 94.2 5.8
and solid wastes generated in the in- Policy, guidelines and regulations on efficient laboratory 3.8 96.2
vestigated laboratories was done by waste management
measuring and recording their respec- Pollution prevention concepts 92.0 8.0
tive weights at the beginning of a work- Public concerns on negative impacts of existing waste 11.5 88.5
ing day on a daily basis for a period of management methods
three months. In some instances where Undergone formal training on chemical waste management 25.0 75.0

Journal of Chemical Health & Safety, November/December 2015 21


expired chemicals and reagents (2) exact amount could not be correctly by the Tanzania Bureau of Standards
used chemicals such as solvents from estimated. Good laboratory waste (TBS) of pH in liquid waste for dispos-
different extraction experiments. management practices prohibit mixing al, which is 6.5–8.5. This indicates
Survey of reasons for the chemicals of unknown chemicals with other che- potential risks to human health and
to be obsolete indicated that most of micals,18 and stipulates correct dispos- the environment due to the release of
them got expired due to long shelf lives al procedures for different types of acidic waste from these laboratories.
(42.3%), some were over-procured wastes, for example the Aldrich Hand- Analysis of samples of liquid waste
(1.9%) and others were missing impor- book19 provides a useful summary of from the investigated laboratories
tant information and thus became un- the correct disposal procedure for revealed the presence of Cr (<0.01–
usable (55.8%). The of chemical waste most chemicals. 0.19 mg/L), Cu (0.02–3. 44 mg/L)
generated in eleven laboratories of the Table 3 summarizes some of the and Pb (0.12–3.22 mg/L) in liquid
six investigated institutions in Dar es physicochemical characteristics of waste from the investigated laborato-
Salaam city were mostly of liquid samples of the liquid waste collected. ries. Concentrations of Cu and Pb in
nature. It shows that the pH values of the some of the laboratories exceeded the
Data on discharge rates of liquid liquid waste measured in the investi- standard set by TBS. The Tanzania
waste indicated that 19.2% of the lab- gated laboratories ranged between 1.2 Bureau of Standards has set a limit
oratories collected 1–20 L/month; and 3.4. This indicates that mostly the of 1.0, 2.0 and 0.1 mg/L for Cr, Cu and
11.5% collected 21–40 L/month and liquid waste discharged was acidic in Pb in chemical waste.20 This indicates
13.5% collected 41–80 L/month. This nature. This is to be expected because potential risks to human health and
variation is due to the different char- this survey also established that the the environmental contamination.
acteristics of the laboratories including chemicals and reagents stocks in the Liquid waste from the other investi-
the kind of activities as well as the laboratories consisted mostly of organ- gated laboratories did not contain
number and nature of experiments car- ic and inorganic acids. These values detectable levels of the measured
ried out and the level of instrumenta- significantly violet the standard set elements.
tion. However in more than half of the
laboratories (55.8%) the discharge
rates for liquid waste could not be Table 3a. Physicochemical Characteristics of Liquid Waste from the Investigated
established due to the absence a proper Laboratories.
of waste collection plan. In such cases Parameter Range Mean  SD Tanzanian Standard20
where there were no temporary waste
pH 1.2–3.4 2.4  0.82 6.5–8.5
storage facilities and instead waste was
Cr (mg/L) <0.01–0.19 0.06  0.07 1.0
discharged directly into the drainage
Cu (mg/L) 0.02–3.44 1.03  1.38 2.0
system upon generation, no estimates
Pb (mg/L) 0.12–3.22 2.05–1.02 0.1
could be made of the amounts.
Studies have indicated that liquid
waste generated in experiments con-
ducted for educational and research
Table 3b. Survey of Waste Handling Practices.
purposes, generally referred to as lab-
oratory waste liquids (LWL) is highly Variable % of
complex because in most cases it con- respondents
tains hazardous chemicals such as (n = 52)
heavy metals.17 Furthermore, although Yes No
all eleven investigated laboratories in-
Obsolete chemicals are removed from laboratories 42.8 57.2
dicated production of solid waste, the
and chemical storage areas
exact average generation rate could
Chemicals are segregated according to their 30.2 68.8
not be established due to several rea-
classification
sons such as inconsistent collection
Waste containers are properly covered 45.5 54.5
method, unavailability of temporary
Waste containers are properly labeled indicating 27.3 72.3
collection facility and in some cases
contents and date
unwillingness of the laboratory per-
Laboratory has a defined waste collection plan in place 25.0 75.0
sonnel to give information, probably
Availability of temporary waste storage facility 75.0 25.0
hesitating to reveal what they well un-
Pre-treatment of chemical waste before disposal 7.7 92.3
derstand to be improper practices. In
Availability of valid transportation contract for 6 94
some cases solid waste from the labo-
chemical waste
ratories was mixed with waste from
Recycling of waste done at the laboratory 15.4 84.6
other institutional sources such as
Waste disposal done by contracted company 3.8 96.2
offices. Mixed wastes that contained
Disposal of liquid waste in drainage system 53.8 46.2
both liquid and solid were also ob-
Disposal of waste by open dumping 42.4 57.6
served in a few cases, but again the

22 Journal of Chemical Health & Safety, November/December 2015


[(Plate_1)TD$FIG]
Assessment of Waste Handling
Practices
Table 3 summarizes results from the
survey of waste handling practices
among the investigated laboratories.
Results of the present study show
obsolete chemicals were not removed
from the laboratories or from the stor-
age areas of more than half of the inves-
tigated laboratories (57.2%). This was
probably due to the lack of monitoring
of the available stock and improper
chemical management practices. Large
stocks of obsolete chemicals were ob-
served in shelves and stores in some of
the investigated laboratories, as demon-
strated in Plate 1. Some of the labora-
tories lacked clear records of waste
collection frequency. Furthermore, in Plate 1. Stocks of obsolete chemicals as was observed in one of the investigated
laboratories’ storage room.
most cases waste were not segregated [(Plate_2)TD$FIG]
according to their nature or classifica-
tion such as whether they are toxic or
non-toxic.
Chemicals used in educational and
research activities are procured by the
institutions from local and internation-
al sources. Data on amounts and spe-
cific sources as well as cases of
donations and gifts from other nations
need to be further collected for a more
comprehensive picture.
The results of our study (Table 3)
indicate that in almost all of the inves-
tigated institutions (>96%) waste col-
lection was not done by a contracted
company. There were no indications of
any systematic method for neither con- Plate 2. Containers of laboratory waste liquids in one of the investigated
taining nor transporting the waste. Of [(Plate_3)TD$FIG]laboratories.
the investigated institutions, none
reported having a contracted company
for waste handling. As such there is no
written proof of how and when waste
is disposed.
Another observation was that in
more than half of the laboratories
(54.5%) liquid waste was collected in
containers with washed-off labels or
no labels at all (72.3%) to indicate
details of the waste such as content,
date of collection and properties, as
shown in Plate 2. Generally, most lab-
oratories (75%) lacked standard oper-
ation procedures of waste collection
and temporary storage as demonstrat-
ed in Plate 3. In these cases laboratory
personnel had to deal with the waste in
whatever way they considered fit for a
specific situation, including disposing Plate 3. Used solvents and other laboratory waste liquids and solids awaiting
them off as ordinary domestic waste. collection on the roof of one of the investigated laboratories.

Journal of Chemical Health & Safety, November/December 2015 23


Pre-treatment procedures that includ- CONCLUSIONS should be conducted to include more
ed neutralization of acidic liquid waste detailed collection of data and investi-
before disposal was applied in only This study is a preliminary assessment gation of other institutions that were
7.7% of the surveyed laboratories. of environmental contamination and not preliminary investigated in the
More than half (53.8%) of the sur- human exposure risks associated with present study.
veyed laboratories discharged their liq- laboratory waste from educational and
uid wastes in the sewerage system. research institutions in Tanzania. ACKNOWLEDGEMENTS
Since it was established that the liquid Results indicate that although most The authors wish to thank Ms. Lilian
waste was mostly acidic in nature of the laboratory technicians con- Dastan Mwasubila for collecting the
(Table 3) this practice is not only a tacted were not formally trained on data. Management teams of the in-
potential risk to the sewerage system laboratory waste management, almost volved institutions are acknowledged
which might be corroded by the mate- all of them were well aware of the risks. for allowing the study to be conducted
rials, but also to the useful microorgan- The assessment of waste handling in their institutions. Technicians and
isms that reside in the final discharge practices revealed that more than half other staff of the eleven laboratories
points of the waste. Although it was of the investigated laboratories did not are appreciated for agreeing to partic-
observed that some harmless soluble keep proper records of the collected ipate in the study and for providing
inorganic salts were permitted to be waste and had no waste collection information. The School of Environ-
discharged in the sewer system, gener- plan. Furthermore, it was revealed that mental Science and Technology,
ally, the surveyed laboratories lacked there exist some improper waste dis- Ardhi University is appreciated for
clear guidelines as to what can or can- posal practices among the investigated providing the laboratory facilities for
not be discharged down the drain, laboratories that include discharge of the analytical work conducted in this
causing some unsuitable materials to untreated waste into sewer systems study.
be discharged as well. A significant per- and open dumping. This is especially
centage of the laboratories (42.4%) ad- alarming because chemical characteri-
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