Amino Sugars Analysis Service

Amino sugars are chemical compounds that have a sugar backbone, in which
one of the hydroxyl groups is replaced by an amine group. Derivatives of
amine-containing sugars, such as N-acetylglucosamine, are also considered
amino sugars. Incorporated into protein-linked sugar chains, amino sugars
regulate protein function and, combined with other compounds, form
antibiotics.

Figure. Amino sugars. The

monosaccharides glucosamine and galactosamine are amino sugars with the amino

group in place of a hydroxyl group.

Amino sugars are widely associated with structural polysaccharides or

glycolipids, and they have been proposed as biomarkers for organic matter
sources and their digenetic state. Three vital amino sugars are of
physiological importance, namely glucosamine, galactosamine and
neuraminic acid. Glucosamine and galactosamine are derived from glucose
and galactose respectively, the amino group in each case replacing the
hydroxyl group on C-2. Neuraminic acid is more complex. It is a nine-carbon
amino sugar formed by the condensation of mannosamine 6-phosphate and
phosphoenolpyruvate (PEP). It is the parent substance of the sialic acids that
are acyl derivatives of neuraminic acid, e.g. N-acetylneuraminic acid. Sialic
acids are found as constituents of glycoproteins and glycolipids and are
believed to have an important bearing on the formation of the acquired
integuments of the teeth.

Figure 2.

The important function of amino sugars.

Several methods for the determination of amino sugars have been published.
Highly specific gas chromatographic analyses require difficult off-line
derivatisation steps, e.g. derivatisation of the hydrolysis products in volatile
aldononitrile acetates. High-performance liquid chromatography (HPLC)
methods need either derivatisation steps or special equipment for anion
exchange chromatography combined with pulsed amperometric detection.
This means that HPLC methods are generally less time-consuming as they
employ no or automated on-line derivatisation. Here Creative Proteomics
 provides highly sensitive and reliable HPLC method for the quantification of
amino sugars.
Platform
 HPLC
Summary
 Identification and quantification of amino sugars.
Report
 A detailed technical report will be provided at the end of the whole project,
including the experiment procedure, instrument parameters.
 Analytes are reported as uM or ug/mg (tissue), and CV's are generally<10%.
 The name of the analytes, abbreviation, formula, molecular weight and CAS#
would also be included in the report.