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Appendix S1
Details of competencies assessed during Levels 1–4 (Years 1–4), and additional
competencies, with corresponding images.
Criteria specified with an asterisk are essential to passing a competency. Missing any specified criterion fails the
competency for a particular image or movie clip.
Image 1:
• Image magnified appropriately.
• Focal zone at appropriate level.
• Ultrasound plane at widest dimensions of gestational sac.
• Gestational sac measured in two orthogonal dimensions using inner-to-inner technique.
Image 2:
• Image magnified.
• Focal zone at appropriate level.
• Ultrasound plane of gestational sac perpendicular to that of Image 1.
• Gestational sac measured at widest dimension using inner-to-inner technique.
• *Mean sac diameter appropriately calculated from mean of all three dimensions from Images 1 and 2.
Figure 1a Gestational sac visualized in sagittal plane of uterus. Note length and height of gestational sac
(measurements 1 and 2, respectively).
Figure 1b Maximum width of gestational sac measured in plane perpendicular to that of Figure 1a. Mean sac
diameter is calculated from mean of length, height (Figure 1a) and width (Figure 1b).
3
Figure 2 Mid-sagittal plane in a fetus at 12 weeks and 5 days, showing crown–rump length (CRL)
measurement.
4
Figure 3a Mid-sagittal plane of uterus in lower uterine segment, showing cephalic presentation (fetal head).
Note mid-sagittal view of the cervix.
Figure 3b Mid-sagittal plane of uterus in lower uterine segment, showing breech presentation (fetal buttocks).
Note mid-sagittal view of the cervix.
5
Table 1D. Fetal extremities
Image 1:
• Right upper extremity, showing hand if possible.
Image 2:
• Left upper extremity, showing hand if possible.
Image 3:
• Right lower extremity, showing foot if possible.
Image 4:
• Left lower extremity, showing foot if possible.
†Fewer than four images are acceptable if both extremities are displayed clearly in a single image.
Figure 4a Ultrasound image of upper fetal extremity. Note appearance of humerus, ulna and radius. Small
portion of radius is shown, due to orientation of upper extremity.
†Caliper may be placed on outer edge of bone based on existing practice patterns.
Passing this competency will also qualify for competency ‘transthalamic plane’ in Table 3B.
Figure 5a Axial plane of fetal head at level of biparietal diameter (BPD), demonstrating falx cerebri, cavum
septi pellucidi (CSP), thalami (T), third ventricle (3 rd V) and insula. Note, BPD measurement in this image is
from outside border of proximal parietal bone to inside border of distal parietal bone.
Figure 5b Same image as in Figure 5a. BPD measurement in this image is from outside border of proximal
parietal bone to outside border of distal parietal bone.
7
Passing this competency will also qualify for competency ‘transthalamic plane’ in Table 3B.
Figure 6 Axial plane of fetal head at biparietal diameter level (transthalamic), demonstrating falx cerebri,
cavum septi pellucidi (CSP), thalami (T), third ventricle (3rd V) and insula. Measurement of head circumference
(HC) is obtained using an ellipse placed on outer edge of cranium. In this case, HC measures 21.74 cm,
corresponding to gestational age of 23 weeks and 6 days.
8
Passing this competency will also qualify for competency ‘abdominal circumference level’ in Table 3H.
Figure 7 Axial plane of fetal abdomen at level of abdominal circumference. Note presence of intra-abdominal
portion of umbilical vein (UV), stomach (St), spine (Sp), descending abdominal aorta (Ao) and inferior vena
cava (IVC). Also note presence of large segments of individual ribs on each side laterally.
9
Figure 8 Longitudinal plane of femur, showing femur diaphysis length (FL) measurement. Note proximal and
distal femoral epiphyses are not yet ossified and not included in FL measurement. Also note presence of distal
femoral spur, which should not be included in FL measurement.
Figure 9 Four images (Q1–Q4), one obtained from each of four quadrants of uterus. Maximum vertical pocket
(MVP) is measured in each quadrant (Q1–Q4). Amniotic fluid index (AFI) is calculated by adding MVP from
all four quadrants. In this fetus, AFI is normal (13.7 cm). MVP is measured in amniotic fluid from top to
bottom in a vertical line.
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Figure 10 Ultrasound image of maximum vertical pocket (MVP) for determination of amniotic fluid volume.
MVP is determined by scanning all four quadrants of uterus and measuring MVP in quadrant with largest
amount of amniotic fluid. MVP is measured in amniotic fluid from top to bottom in a vertical line.
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• Transvaginal ultrasound.
• Mid-sagittal plane of cervix.
• Focal zone at appropriate level.
• Image magnified appropriately.
• Anterior cervical width is equal to posterior cervical width.
• Maternal bladder empty.
• Internal os seen.
• External os seen.
• Cervical canal visible throughout.
• Caliper placement correct.
Figure 11 Mid-sagittal plane of cervix obtained by transvaginal approach. Cervical length is measured from
internal cervical os to external cervical os (3.8 cm in this example). Note anterior position of maternal bladder
and presenting fetal head.
13
• Transabdominal ultrasound.
• Focal zone at appropriate level.
• Image magnified appropriately.
• Mid-sagittal plane of cervix.
• Maternal bladder non-distended.
• Internal os seen.
• External os seen.
• Caliper placement correct.
Figure 12 Mid-sagittal plane of cervix obtained by transabdominal approach. Cervical length is measured from
internal os to external cervical os (4 cm in this example). Fetal head is presenting. Note location of vaginal
canal. Distention of maternal bladder may impact measurement of cervical measurement on transabdominal
approach.
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Table 2C. Endometrial thickness
• *Transvaginal ultrasound.
• Focal zone at appropriate level.
• Mid-sagittal plane of cervix and uterus, with magnification of endometrium as needed for endometrial
measurement.
• Endometrial lining (echo) seen in its entirety from cervical canal to endometrial fundus.
• Cul-de-sac imaged posterior to cervix.
• Calipers placed in anteroposterior orientation, perpendicular to long axis of uterus, using outer-to-outer
technique†.
• Widest endometrial thickness measured using outer-to-outer technique.
†In presence of endometrial fluid, measure anterior and posterior endometrial walls separately and add the two
measurements.
Figure 13 Mid-sagittal plane of uterus obtained for measurements of endometrial thickness. Note midline
endometrial lining (echo) is seen in its entirety from cervical canal to endometrial fundus. Measurement of
endometrial thickness is obtained by placing calipers in an anteroposterior orientation, perpendicular to long
axis of uterus and using outer-to-outer technique.
15
• Transvaginal ultrasound.
• Focal zone at appropriate level.
• Image magnified appropriately.
Image 1:
• Mid-sagittal plane of cervix and uterus.
• Endometrial lining seen in its entirety from cervical canal to endometrial fundus.
• Cul-de-sac imaged posterior to cervix.
• Length of uterus measured from fundus to external os using outer-to-outer technique†. An alternate method
for measurement of uterine length includes measurement from fundal region, along endometrial lining and
endocervical canal, using outer-to-outer technique.
• Anteroposterior diameter of uterus measured from anterior to posterior serosal surface at widest dimension
perpendicular to long axis of uterus using outer-to-outer technique.
Image 2:
• Transverse (axial) plane of uterus at widest dimension.
• Transverse diameter of uterus measured from left to right serosal surface at widest dimension using outer-
to-outer technique.
†Morphological Uterus Sonographic Assessment (MUSA) is another method that can be followed/used for
uterine measurement13.
Passing this competency will also qualify for competency ‘Transvaginal pelvic examination: uterus’ in Table
2F.
Figure 14a1 Mid-sagittal plane of uterus obtained for measurements of uterine length and height. Note
endometrial lining (echo) is seen in its entirety from cervical canal to endometrial fundus. Length of uterus
(measurement 1 in this image) is measured from fundus to outer edge of cervix. In this mid-sagittal plane,
anteroposterior diameter of uterus is also measured at widest dimension (measurement 2 in this image),
perpendicular to long axis of uterus.
16
Figure 14a2 Mid-sagittal plane of uterus obtained for measurement of uterine length and height. Note
endometrial lining (echo) is seen in its entirety from cervical canal to endometrial fundus. Length of uterus
(measurement 1 in this figure) is measured from fundus to outer edge of cervix, following endometrial lining
and endocervical canal, using outer-to-outer technique. This technique of uterine measurement allows for more
accuracy in presence of uterine flexion. In this mid-sagittal plane, anteroposterior diameter of uterus is also
measured at widest dimension (measurement 2 in this figure), perpendicular to long axis of uterus.
Figure 14b Transverse plane of uterus, shown at its widest dimension. Uterine width is measured from left to
right at widest uterine dimension.
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Image 1:
• Plane of pelvis showing ovary with clear outline in longest dimension.
• Length of ovary measured from anterior to posterior at longest dimension.
• Anteroposterior diameter of ovary measured from left to right, orthogonal to length measurement.
Image 2:
• Plane of pelvis showing ovary with clear outline at right angles to Image 1.
• Width of ovary measured from left to right at widest dimension.
Passing this competency will also qualify for competency ‘Transvaginal pelvic examination: ovaries’ in Table
2G.
Figure 15a Longitudinal plane of ovary, showing measurements of ovarian length and anteroposterior
diameter. Anteroposterior diameter is measured perpendicular to length measurement.
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Figure 15b Axial plane of ovary, perpendicular to longitudinal plane (Figure 15a), showing ovarian width
measurement.
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• Transvaginal ultrasound.
• Focal zone at appropriate level.
• Image magnified appropriately.
Competency for this plane can also be established as part of uterine measurement competency in Table 2D.
See Figure 14.
Image 1:
• Parasagittal plane of pelvis showing ovary with clear outline in longest dimension.
Image 2:
• Axial plane of pelvis showing ovary with clear outline at right angles to Image 1.
Competency for this plane can also be established as part of ovarian measurement competency in Table 2E.
See Figure 15.
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Figure 16 Axial view of fetal head at level of transventricular plane. Note atrium and occipital horns of lateral
ventricle and choroid plexus within. Measurement of lateral ventricle is obtained at the atrium, at level of
parieto-occipital groove. Calipers are placed on medial and lateral walls, perpendicular to long axis of
ventricle, using inner-to-inner technique. Although cavum septi pellucidi (CSP) is seen in this figure, it does
not have to be visualized as, on many occasions, the lateral ventricle can be seen in a plane that is superior to
the CSP.
21
Competency for this plane can also be established as part of biparietal diameter/head circumference
measurement competencies, as shown in Tables 1E and 1F.
See Figure 5.
Figure 17 Transcerebellar plane. Angled axial plane of fetal head, showing posterior fossa. Note symmetric
appearance of cerebellar hemispheres, cerebellar vermis and cisterna magna. In this plane, falx cerebri, cavum
septi pellucidi (CSP) and thalami (T) are also seen.
22
Table 3D. Face: upper lip and philtrum
Figure 18 Coronal view of fetal face, showing upper lip and philtrum. Note appearance of soft tissue of upper
lip, philtrum and nares.
23
Table 3E. Four-chamber view
Figure 19 Axial view of fetal chest at level of four-chamber view of heart. Note appearance of the four
chambers with right ventricle (RV) as most anterior chamber, and left atrium (LA) as most posterior chamber.
Left ventricle (LV) and right atrium (RA) are also seen. Note location of descending thoracic aorta (Ao), and
spine (Sp) posteriorly. Note that apex of heart is towards fetal left side and also note presence of large
segments of individual ribs.
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Figure 20 Angled axial plane of fetal chest, showing left ventricular outflow tract and aorta (Ao). Note Ao
arising from left ventricle (LV), and continuity of Ao with ventricular septum. LA, left atrium; RA, right
atrium; RV, right ventricle.
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Figure 21 Transverse plane of fetal upper chest at level of right ventricular outflow tract. Note pulmonary
artery (PA), superior to the aorta (Ao). Also note pulmonary valve (PV).
Competency for this plane can also be established as part of abdominal circumference measurement
competency, as shown in Table 1G.
See Figure 7.
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Figure 22a Axial plane of fetal abdomen at level of kidneys. Note right and left kidneys and renal pelves,
imaged in posterior aspect of fetal abdomen, lateral to fetal spine.
Figure 22b Coronal plane of fetal posterior abdomen. Note presence of right and left kidneys in coronal view.
27
Figure 22c Sagittal plane of right side of abdomen, showing right kidney.
Figure 23 Axial plane of fetal abdomen at level of cord insertion. Note insertion of umbilical cord into anterior
abdominal wall.
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Figure 24a Axial plane of fetal pelvis (color Doppler) at level of fetal bladder. Note presence of two umbilical
arteries, lateral to bladder.
Figure 24b Axial plane (grayscale) of free loop of umbilical cord in amniotic fluid, showing umbilical vein and
two umbilical arteries.
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Figure 24c Axial plane (color Doppler) of free loop of umbilical cord in amniotic fluid (as in Figure 24b),
showing umbilical vein and two umbilical arteries. Note that blood flow in umbilical vein is in reverse direction
to that of umbilical artery.
Figure 25 Axial plane of fetal lower abdomen and pelvis, showing fetal bladder in its anterior position. In this
axial plane, cross-sections of femora are noted in lower extremity.
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Image 1:
• *Entire length of cervical spine evaluated.
Image 2:
• *Entire length of thoracic spine evaluated.
Image 3:
• *Entire length of lumbosacral spine evaluated.
Figure 26a Mid-sagittal plane of fetal spine at level of cervical and thoracic region. Note that entire length of
cervical and thoracic spine is shown, with normal overlying skin and no spinal abnormalities. Spinous process
and corresponding vertebral body is seen for each vertebra.
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Figure 26b Mid-sagittal plane of fetal spine at level of thoracic region. Note that entire length of thoracic spine
is shown, with normal overlying skin and no spinal abnormalities. Spinous process and corresponding vertebral
body is seen for each vertebra.
Figure 26c Mid-sagittal plane of fetal spine at level of lumbosacral region. Note that entire length of
lumbosacral spine is shown, with normal overlying skin and no spinal abnormalities. Spinous process and
corresponding vertebral body is seen for each vertebra.
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Table 3N. Spine: axial
Image/Sweep 1:
• *Entire length of cervical spine evaluated.
Image/Sweep 2:
• *Entire length of thoracic spine evaluated.
Image/Sweep 3:
• *Entire length of lumbosacral spine evaluated.
Figure 27a Axial plane of fetal spine at level of cervical spine. Note normal orientation of spinal lateral
processes and normal overlying skin.
33
Figure 27b Axial plane of fetal spine at level of thoracic spine. Note normal orientation of spinal lateral
processes and normal overlying skin.
Figure 27c Axial plane of fetal spine at level of lumbosacral spine. Note normal orientation of spinal lateral
processes and normal overlying skin. Iliac bones are seen in the pelvis laterally on each side.
34
Basic information:
• * Presence or absence of cardiac activity.
• *Location of gestational sac.
• *Number of fetuses.
• *Location of fetuses in multiple pregnancy.
• *Placental location.
• *Type of placentation in multiple pregnancy.
• *Assessment of amniotic fluid.
• *Fetal lie and presentation.
Fetal anatomy:
• Described appropriate to setting and resources.
• *Basic anatomy.
• Detailed anatomy.
• *Estimated gestational age based on established guidelines.
• *Estimated fetal weight (after 24 weeks).
• *Summary of examination and comments.
• Comparison with previous studies.
• Limitations of ultrasound examination.
• Recommendations for follow-up if necessary.
35
Biometric information:
• *Uterine height, length and width.
• *Ovarian measurements in three dimensions are a required part of imaging, but not of written report.
• *Cul-de-sac: fluid or abnormalities.
Abnormalities:
• *Uterine.
• *Adnexal.
• *Cul-de-sac.
• *Surrounding pelvic structures.
Figure 28 Mid-sagittal plane of fetal face, showing facial profile. Note tip of nose, nasal bone, maxilla and
mandible.
Procedure
• Timeout before procedure.
• Fetal viability established before procedure.
• Needle insertion performed under ultrasound guidance.
• Transplacental puncture avoided if possible.
• Needle gauge 22–20.
• Maximum of two insertions.
• Volume of amniotic fluid retrieved as needed for test.
• Fetal wellbeing: assessment of fetal heart rate after procedure.
• Rhogam administered if indicated.
37
5. Additional competencies
Additional competencies listed here are not currently part of the basic obstetric and gynecologic ultrasound
examination. Programs may opt to add these competencies as part of ultrasound training.
Figure 29a Angled axial plane of fetal upper chest, showing three vessels and trachea view. Note anterior
location of pulmonary artery (PA), with ductus arteriosus (DA) connecting with descending aorta (Descending
Ao). Aorta (Ao) and aortic isthmus (Ao Isthmus) are also seen connecting with descending Ao. Superior vena
cava (SVC) is seen in cross-section to right side of aorta. Note that DA and Ao isthmus are to left side of
trachea, confirming presence of normal left aortic and ductal arches. Spine (Sp) is seen posteriorly.
38
Figure 29b Angled axial plane of fetal upper chest, showing three vessels and trachea view with color Doppler.
Note that color Doppler shows blood flow in both ductal arch (DA) and aortic isthmus (Ao Isthmus) towards
descending aorta (Descending Ao). Ao, aorta; PA, pulmonary artery; Sp, spine; SVC, superior vena cava.
Figure 31 Color and pulsed Doppler of umbilical artery obtained at level of placental cord insertion. Note that
Doppler waveforms show forward flow during diastole (D). Note also peak of waveforms, corresponding to
peak systole (S).
40
Figure 32 Mid-sagittal plane of fetus in first trimester of pregnancy, showing measurement of nuchal
translucency (NT). Note in this mid-sagittal plane, tip of nose, maxilla and mid brain are all visible. Also note
that amnion is seen as a separate membrane. Measurement of NT is performed by placement of calipers at
widest NT space, using ‘on-to-on’ technique.
41
Procedure:
• Timeout performed before procedure.
• Patient in supine position.
• Transvaginal ultrasound used.
Image criteria:
• Long-axis view of endometrial cavity during fluid injection.
• Adequate endometrial cavity distension (probe pressure at internal os in sagittal orientation to maintain
distension).
• Three-dimensional volume if available: sagittal, transverse and coronal views.
• Two-dimensional views:
• Sagittal view: to include entire endometrial cavity.
• Transverse view: fundus, mid-uterine and lower-uterine segments.
• Real-time evaluation of endometrial cavity with targeted image capture.
• Endometrial wall thickness: anterior and posterior walls measured separately.
• Define/describe endometrium: global/uniform, focal irregularity (mass, polyp or fibroid).
Figure 33a Mid-sagittal plane of uterus obtained during saline contrast sonohysterography. Note distended
endometrial cavity and sonohysterography catheter in isthmic portion of uterus. Note that cavity is normal
without any visible abnormality.
42
Figure 33b Three-dimensional (3D) ultrasound volume of uterus obtained during sonohysterography. Note
transverse plane in A, sagittal plane in B, and coronal plane in C. 3D coronal plane is also displayed in lower
right quadrant. Note that coronal planes in two-dimensional and 3D images show normal endometrial cavity
without any abnormality.