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(EC 3.4.22.32)
PRINCIPLE:
Bromelain
Gelatin + H2O > Amino Acids + Oligopeptides
METHOD: Titrimetric
REAGENTS:
PEGELA01 Page 1 of 3
Revised: 01/16/98
Enzymatic Assay of BROMELAIN
(EC 3.4.22.32)
REAGENTS: (continued)
Test Blank
N = Normality of Reagent D
14 = mg Nitrogen per millimole Nitrogen
1000 = milligrams/gram
PEGELA01 Page 2 of 3
Revised: 01/16/98
Enzymatic Assay of BROMELAIN
(EC 3.4.22.32)
UNIT DEFINITION:
This procedure is for informational purposes. For a current copy of Sigma’s quality control
procedure contact our Technical Service Department.
PEGELA01 Page 3 of 3
Revised: 01/16/98