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Blood Collection in Swine
M. Michael Swindle, DVM, Professor and Chairman, Department of
bio-resources Comparative Medicine, Medical University of SC, Charleston, SC 29425
Recommended administration volumes (ml/kg) are as for the cephalic, coccygeal and auricular veins. Alcohol
follows: IM-0.05-0.5, IP-10.0-20.0, PO-10-15, SC-2, applied to the skin over the superficial veins will facilitate
IV bolus-2.5-5.0, IV infusion 5.0-15.0 mg/kg/hr. Various the identification and dilation of the vessels.
vessels are amenable to collection of different volumes
of blood due to their size and location and are discussed Cranial Vena Cava: The cranial vena cava (precava) is
below. Recommended needle sizes are also discussed the largest vessel that is commonly accessed for blood
for each blood vessel. samples. The sample size from this vessel is only
limited by the amount of blood which can be safely
removed without causing harm to the animal.
Restraint for Venipuncture Animals may be positioned in a restraint
Agricultural methods of restraint such as snout tying and sling or placed in dorsal recumbency
squeeze chutes should be avoided in research swine. to access this vessel. The vessel
These methodologies are stressful to the pig and make is always accessed from
it more difficult for personnel to interact with the animals the right side of the
when they are on a chronic study. Vocalization during
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info@sinclairbioresources.com
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PO Box 658 Columbia, MO 65205
Tel: (573)387-4400 ▪ Fax: (573)387-4404
Sample Collection Series
sinclair
Blood Collection in Swine
M. Michael Swindle, DVM, Professor and Chairman, Department of
bio-resources Comparative Medicine, Medical University of SC, Charleston, SC 29425
thoracic inlet to avoid the vagus and recurrent laryngeal artery and the nerve. The carotid artery is frequently
nerves which are more prominent on the left side. The used as the vascular access site for terminal bleeding
needle is advanced at a 450 angle towards the left under general anesthesia. These vessels can be
shoulder of the pig. Repeated blood sampling from accessed with a 20 g, 1.0-1.5” needle. Bradycardia or
this area will result in hematoma or blood clot formation arrhythmias may occur if the vagus nerve is accidentally
in the thoracic inlet because it is not possible to stop stimulated with the needle. (Figure 3)
leakage from the venipuncture with digital pressure. In
swine <50 kg the vessel can be accessed using a 20 g,
1.5” needle. For animals >50 kg needles as large as 18
g, 2.5” may have to be used. (Figure 1)
Additional Reading
In addition to the references listed below, this bulletin
is complementary to other bulletins and fact sheets
© 2010 Sinclair BioResources, LLC. All Rights Reserved.
www.sinclairbioresources.com
info@sinclairbioresources.com
4
PO Box 658 Columbia, MO 65205
Tel: (573)387-4400 ▪ Fax: (573)387-4404