GROUP 8

Guiawan, Rishayle Mae M.

Maquera, Kricel-Mae M.

Pascua, Glydel G.

Silva, Julie Anne A.

Tuliao, Jon Yoshe S.

2.1. In order to measure the enzyme activity and the initial rate of reaction, 5 mL of cellobiose
(100mumol/mL) ad 44 mL of buffer solution were placed in a stirred vessel. The reaction was
initiated by adding 1 mL of enzyme (beta-glucosidae) solution which contained 0.1 mg of protein
per mL. At 1, 5, 10, 15, and 30 minutes, 0.1 mL of sample was removed from the reaction mixture
and its glucose content was measured. The results were as follows:

Time Glucose Concentration

min µmol/mL
1 0.05
5 0.23
10 0.38
15 0.52
30 1.03

a. What is the activity of the β-glucosidae in units /mL of enzyme solution and in unit’s /mg
protein? A unit is defined as the enzyme activity which can produce Imumol of product per
minute.
b. What is the initial rate of reaction?
SOLUTION:

Total volume = 44 + 5 + 1 =50mL

From the graph the equation obtained:
y=0.033x + 0.0391
m=0.033mumol/mL.min

a. Activity of the β-glucosidae

0.033 x 50 = 1.65 mumol/min

i)

1.65𝑚𝑢𝑚𝑜𝑙 0.1𝑚𝑔
= ÷
𝑚𝑖𝑛 𝑚𝑙𝑥0.1𝑚𝑙

𝒖𝒏𝒊𝒕𝒔
= 𝟏𝟔𝟓
𝒎𝒈 𝒑𝒓𝒐𝒕𝒆𝒊𝒏

ii)

1.65𝑚𝑢𝑚𝑜𝑙
= ÷ 1𝑚𝑙 𝑜𝑓 𝑒𝑛𝑧𝑦𝑚𝑒
𝑚𝑖𝑛

𝒖𝒏𝒊𝒕𝒔
= 𝟏. 𝟔𝟓 𝒐𝒇 𝒆𝒏𝒛𝒚𝒎𝒆
𝒎𝒍
b. Initial rate of reaction 0.033 mumol/mL.min