BIOLOGY TEST FORM 4
NAME:……………………………………..
SECTION A
Choose one answer and write in the grid provided at the
end of Section A.
1. The diagram shows six cells.
Which are plant cells and which are animal cells?
2. Which series of terms is listed in order of increasing
level of organisation?
3. Which is a tissue and which is an organ?
4. A cell is observed under a microscope.
Which feature identifies it as a plant cell?
A. The cell contains a single large sap vacuole.
B. The cell contains glucose and amino acids.
C. The cell contains stored fat.
D. The cell surface membrane is partially
permeable.
TIME : 1 hour
5. The diagram shows a specialised cell.
For which function is the cell adapted?
A absorption of water
B contraction of muscles
C movement of dust particles
D transport of oxygen
6. The diagram represents two liquids, separated by a
membrane through which osmosis can occur.
What movement of molecules will occur?
A. Molecules of dissolved substance move from
left to right.
B. Molecules of dissolved substance move from
right to left.
C. Overall, water molecules move from left to right.
D. Overall, water molecules move from right to left.
7. The diagrams show an experiment when set up and
the same experiment two hours later.
What explains the movement of water and dye?
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8. Which diagram shows the appearance of a plant 13 Which feature indicates that a root cell is from a plant
cell several minutes after it has been placed in a and not an animal?
concentrated solution of sugar? A. cell membrane
B. cell wall
C. chloroplast
D. cytoplasm

14 Which process needs energy from respiration?

A. movement of carbon dioxide into the alveoli
B. movement of oxygen into red blood cells
C. uptake of glucose by cells in the villi
D. uptake of water by root hair cells

15 The diagram shows the result of an experiment. The

liquid in the glass tube had risen to point X after three
hours.
9. The diagrams show a leaf cell before and after
staining with iodine solution.

Which substance causes the iodine solution to change

colour?
A chlorophyll
B protein
C reducing sugar In a second experiment, which change could be made
D starch to cause the liquid to rise higher than X?
A. a larger beaker
10 What are enzymes? B. a smaller bag
A. fats that are secreted by glands in the digestive C. water in the bag
system D. 0.4 mol / dm3 sucrose solution in the bag
B. fats that have a characteristic molecular shape
proteins that act as biological catalysts 16 Which property of enzymes is explained by the lock
C. proteins that are unaffected by temperature and key hypothesis?
A. All enzymes are proteins.
11 Which statements about osmosis are correct? B. Enzymes are inactive at very low temperatures.
1. Glucose molecules can move by osmosis. C. Human enzymes are most active just below 40
oC.
2. Molecules pass through a partially permeable
membrane. D. Most enzymes can only catalyse one reaction.
3. Molecules move down a water potential
gradient. 17 In an enzyme’s action, where is the active site and
A. 1, 2 and 3 where are the lock and the key?
B. 1 and 2 only
C. 1 and 3 only
D. 2 and 3 only

12 Enzyme action can be explained by the lock and key

hypothesis.
Where is the active site and does the substrate act as
the lock or as the key?

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18 A student takes a potato and cuts three pieces from
it. Each piece is 5 cm X 0.5 cm X 0.5 cm. He places the
three potato pieces into three different sugar solutions.

After two hours, he removes the potato pieces from the

sugar solutions and measures their lengths.

The results are shown in the table.

What can be concluded from these results?

A. Solution Y has a lower water potential than the
potato.
B. Solution Z has the lowest water potential.
C. The potato piece in solution X increases in
length because it takes up sugar.
D. The potato piece in solution Y decreases in
length because it loses sugar.

19 Which movement of a substance in plants is an

energy-consuming process?
A. absorption of carbon dioxide by a palisade cell
B. loss of water into the air spaces of a leaf
C. nitrate uptake by root hair cells
D. transport of water up through the xylem

20 The diagram represents an enzyme molecule.

What could be substrates for this enzyme?

A. W only
B. W or X
C. Y only
D. Y or Z

ANSWERS GRID
1 6 11 16
2 7 12 17
3 8 13 18
4 9 14 19
5 10 15 20

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 SECTION B
1. Write your answers in the space provided.

Fig. 1 Fig. 2

Fig. 1 shows a plant cell at the start of an experiment. Fig. 2 shows the plant cell after a few minutes.
(a) Identify the change that has taken place.
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(b) Explain what has happened to cause the change above.
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(c) Suggest a liquid which surrounds the cell during the experiment.
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(d) What condition is shown by the cell shown in Fig. 2?
(e) ...........................................................................................................................................................................[1]
[Total: 5 marks]

2 Enzymes are used commercially to extract juice from apples.

Fig. 2.1 shows two containers of apple juice. One contains juice extracted using an enzyme and the other without an
enzyme.

Fig. 2.1
(a) With reference to Fig. 2.1, compare the volume and appearance of the two juices extracted.
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Some students investigated the effect of pH on the production of apple juice using the same enzyme.
 The apples were chopped up and formed into a smooth pulp.
 The pulp was divided into samples. Each sample was adjusted to a different pH.
 Enzyme was stirred into each sample of pulp and left to stand for 10 minutes for the enzyme to react.
 The mixtures of pulp and enzyme were then filtered for 20 minutes to collect the juice. The volumes of apple juice
collected, are shown in

Table 2.1.
pH volume of apple juice
collected / cm3
3 40
4 56
5 95
6 60
7 30

(b) (i) Construct a graph to show the effect of pH on the production of apple juice using this enzyme.

[5]

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(ii) State the optimum pH for the action of this enzyme.
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(iii) Describe and explain the effect of pH on the production of apple juice, using this enzyme.
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(c) Suggest the factors that need to be controlled in this investigation.
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[Total: 15]

3 (a) With reference to named components, describe how the structure of one animal cell (for example from fresh liver)
would appear different from a plant cell (for example from an onion epidermis).
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(b) State the relationship between structure and function for both of the following:
xylem vessels
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red blood cells
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[Total: 10]