PERHITUNGAN

A. Pembuatan Reagen
1. Amonium Persulfat 1,31 mol/L
𝑔𝑟 1000
M = x
𝑀𝑟 𝑣

𝑔𝑟 1000
1,31 = x
228,18 15

Gr = 4,4837 gram

2. Asam Arsenat 0,05 mol/L

𝑔𝑟 1000
M = x
𝑀𝑟 𝑣

𝑔𝑟 1000
0,05 = x
197,841 15

Gr = 0,1483 gram

3. Ceric Ammonium Sulfat 0,019 mol/L

𝑔𝑟 1000
M = x
𝑀𝑟 𝑣

𝑔𝑟 1000
0,019 = x
632,56 10

Gr = 0,12 gram

4. Asam Sulfat 1,75 mol/L 98%

𝑔𝑟 1000
M = x
𝑀𝑟 𝑣

98 1000
M = x
98 100

M = 10
V1 x N1 = V2 x N2
V1 x 10 = 10 x 1,75
V1 = 1,75 ml
 5. NaOH 0,875 mol/L
𝑔𝑟 1000
M = x
𝑀𝑟 𝑣

𝑔𝑟 1000
0,875 = x
40 10

Gr = 0,35 gram

6. NaCl
12,5 𝑔𝑟 𝑥
x
500 𝑚𝑙 15 𝑚𝑙
X = 0,375 gram

7. KIO3 78,8 mmol (10000 ppm Iodium)

𝑚𝑔 1000
78,8 mmol = x
214 10𝑥

Mg = 168,6 mg dalam 10Ml

B. Pengenceran Larutan Stok

10.000 ppm stok menjadi 1000 ; 500 ; 250 ; 125 ; 62,5 ; 31,25 ; 15,625 ppm
 10.000 x V1 = 1000 x 10
V1 = 1 ml, ad 9 ml aquadest
 10.000 x V1 = 500 x 10
V1 = 0,5 ml , ad 9,5 ml aquadest

Dari stok 1000 ppm

 1000 x V1 = 250 x 10
V1 = 2,5 ml , ad 7,5 ml
 1000 x V1 = 125 x 10
V1 = 1,25 , ad 8,75 ml

Dari stok 500 ppm

 500 x V1 = 62,5 x 10
V1 = 1,25ml , ad 8,75 ml
 500 x V1 = 15,625 x 10
 V1 = 0,3125 ml , ad 9,68 ml

C. Hasil kadar Iodin dalam urin

Konsentrasi Absorbansi
62,5 ppm 0,142
31,25 ppm 0,249
15,625 ppm 0,251

Chart Title
0.3
0.25
Absorbansi

0.2
y = -0.0025x + 0.3045
0.15
R² = 0.9026 Series1
0.1
Linear (Series1)
0.05
0
0 20 40 60 80
Konsentrasi

D. Hasil Absorbansi Kelompok

NPM Absorbansi  36 (E5) , A = 0,102

Y = -0,0025x + 0,3045
36 (E5) 0,102
0,102= -0,0025x + 0,3045
37 (E6) 0,199 X = 81 ppm
38 (E7) 0,195
 37 (E6) , A = 0,199
40 (E8) 0,113 Y = -0,0025x + 0,3045
0,199 = -0,0025x + 0,3045
X = 42,2 ppm

 38 (E7) , A = 0,195
Y = -0,0025x + 0,3045
0,199 = -0,0025x + 0,3045
X = 83,4 ppm

 40 (E8) , A = 0,113
Y = -0,0025x + 0,3045
0,113 = -0,0025x + 0,3045
X = 76,6 ppm
Normal iodin (Referensi jurnal ) > 0,79 µmol/L

Mr Iodin = 253,809 gr/mol

0,79 µmol/L = 0,00079 mmol/L

0,00079 µmol/L x 253,809 gr/mol = 0,2 mg/L = 0,2 ppm

Nilai normal Iodin > 0,2 ppm

Normal Iodin (referensi lain) = 0,1 – 0,3 ppm
Resiko hipertiroid > 0,3 ppm