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Chapter 1
ENZYMES
Contents in Chapter 1
1.1 The general characteristics of enzymes
1.2 The relationship between enzyme and activation energy
1.3 Enzyme specificity relating to Key and Lock Model
1.4 Enzyme specificity relating to Induced-Fit Model
1.5 Factors affecting enzyme activities
-Enzyme concentration, substrate concentration, temperature, pH
1.6 Inhibition
-Basic principle of enzyme inhibition (competitive versus non-
competitive; reversible versus irreversible)
- One example of competitive and non-competitive inhibition
- One example of reversible and irreversible enzyme inhibition
1.7 Allosteric regulation
- Definition of allosteric activator and inhibitor
- Allosteric inhibition
1.8 Enzyme Classification (6 classes)
- Oxidoreductase, Transferase, Isomerase, Hydrolase, Lyase, and
Ligase
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Chapter 1: Enzymes
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METABOLISM
• The total of all the chemical reactions occur in
cells is known as metabolism.
• These chemical reactions may be classified
into two types:
- anabolism
- catabolism
METABOLISM
– sum of all the chemical reactions that occur within an organism
ANABOLISM CATABOLISM
- Simpler substances combined to form - Complex substances are broken
more complex substances. down to form simpler substance.
- Eg; - Eg;
synthesis of glycogen hydrolysis of glycogen
(glucose + glucose)n glycogen Breakdown of glycogen to glucose
unit
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Characteristics of enzyme
1. Enzymes globular protein with are catalytic properties.
made a long and linear chains of amino acids that fold to
produce 3-D shape.
mostly tertiary and quaternary structure.
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Characteristics of enzyme
3. Reaction reversible
catalyzing reaction of enzyme is reversible and does not
consumed by the reaction.
4. Enzyme specificity
For any chemical reaction, the reactant that an enzyme acts on
is called the substrate.
Highly specific in their action, only certain substrate will fit into
enzyme’s active site.
The enzyme binds to its substrate, forming an enzyme-substrate
complex.
Characteristics of enzyme
• Enzymes bind only certain substrate.
• Absolute specificity
- catalyze one reaction
• Group specificity
- act only on specific
functional group such as H20
Sucrase
amino, methyl and
phosphate
• Linkage specificity
- act on particular type of
chemical bond
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Characteristics of enzyme
5. Very efficient
Only small amount of enzyme can change a large amount of
substrate into products.
7. Catalysts
because they can speed up chemical reactions by Lowering the
Activation Energy.
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delta G / ∆G
The different
between free
energy.
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(G 0)
energy is released. Energy
Products
- breakdown of high energy
substances low energy
product Progress of the reaction
(G 0)
complex substances Energy
Reactants
Energy coupling – Energy
generated by catabolic process
used by cells to perform anabolic Progress of the reaction
process.
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Mode of action/Mechanism of
enzyme action
Induced-Fit Model
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