Escolar Documentos
Profissional Documentos
Cultura Documentos
Fig. S1. Differential Interference Contrast Microscopy (DIC) of the 3 cell types in
oyster hemolymph. A. Agranulocytes compared to REF Granulocytes.
Agranulocytes have a flattened appearance, possess little or no granules and
move very slowly. In contrast, highly motile REF granulocytes appear as
brilliantly lit cells that contain cubodial birefringent granules. B. Granulocytes
compared to REF Granulocytes. In contrast to REF granulocytes, the highly
motile granulocytes have a fan shaped appearance and contain numerous
relatively non-refractile spherical granules.
Fig S4. EDS spot analysis of crystal bearing hemocyte on the surface of
prismatic shell. A. The individual prisms of the prismatic layer beneath the cell
are visible. B. S-1, a spot on the crystal contains 1.34 times more calcium then
spot S-2, a spot on the glass slide. This is a lower ratio then figure S3 due to the
fact that the underlying surface is calcified shell.
Fig S5. Pseudocolor SEM of crystal-bearing hemocytes on the surface of newly
formed prismatic shell. Shell pieces were fixed in 4% paraformaldehyde
immediately after collection. A. Individual prisms (pink) appear to be enclosed in
organic matrix (green). B. SEM of box in A. A hemocyte is visible on surface of
an individual prism. Intracellular crystals are yellow. In contrast to the cells, the
organic matrix surrounding the prisms appears to be devioid of crystals. 5 µm
scale bar.
SUPPLEMENTARY http://science.sciencemag.org/content/suppl/2004/04/07/304.5668.297.DC1
MATERIALS
RELATED file:/contentpending:yes
CONTENT
REFERENCES This article cites 22 articles, 3 of which you can access for free
http://science.sciencemag.org/content/304/5668/297#BIBL
PERMISSIONS http://www.sciencemag.org/help/reprints-and-permissions
Science (print ISSN 0036-8075; online ISSN 1095-9203) is published by the American Association for the Advancement of
Science, 1200 New York Avenue NW, Washington, DC 20005. 2017 © The Authors, some rights reserved; exclusive
licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. The title
Science is a registered trademark of AAAS.