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Storage and sampling Table 1—Growth of background bacterial populationsa as affected by treatments in vacuum-
packaged pre-peeled potatoes stored at 4°C
The inoculated vacuum-packaged potato
samples were stored at 4°, 15° and 28°C. Treatment Day
Samples stored at 4°C were analyzed on days 0 8 14 21
0, 8, 14, and 21. Samples stored at 15°C were Aerobic Sulfite 2.82 ± 0.15 6.36 ± 0.31 6.31 ± 0.36 6.27 ± 0.28
analyzed on days 0, 3, 6, 9, and 12; and those Plate CBI 2.82 ± 0.15 6.29 ± 0.26 6.92 ± 0.27 7.74 ± 0.14
stored at 28°C were analyzed at 0, 4, 8, 12, Counts CBI-RINSE 2.82 ± 0.15 6.27 ± 0.37b 6.22 ± 0.14b 6.13 ± 0.25b
Control 2.82 ± 0.15 6.46 ± 0.21 7.65 ± 0.37b 9.60 ± 0.28b
18, 24, 48, and 72h. Samples were observed
for visual evidence of spoilage or presence Anaerobic Sulfite 2.69 ± 0.22 6.74 ± 0.15 6.47 ± 0.28 5.53 ± 0.28
of off-odor. Plate CBI 2.69 ± 0.22 6.29 ± 0.40 5.67 ± 0.16 7.54 ± 0.17
Counts CBI-RINSE 2.69 ± 0.22 7.83 ± 0.26 6.40 ± 0.36 5.99 ± 0.33
Control 2.69 ± 0.22 7.46 ± 0.36 8.28 ± 0.28 8.91 ± 0.32
Bacterial enumeration
At the scheduled sampling time, sterile CVT Sulfite 2.47 ± 0.19 7.29 ± 0.23 8.32 ± 0.43 5.25 ± 0.16
0.1% peptone water (w/v) was added to each Plate CBI 2.47 ± 0.19 6.48 ± 0.23 7.34 ± 0.26 7.68 ± 0.25
Counts CBI-RINSE 2.47 ± 0.19 7.41 ± 0.15 7.75 ± 0.33 7.95 ± 0.27
bag to give a 1:1 (w/v) dilution and the con- Control 2.47 ± 0.19 7.31 ± 0.27 7.86 ± 0.14 8.89 ± 0.32
tents were homogenized for 1 min with a a All plate counts are log
10 CFU/g and represent means ± standard deviations of two replications.
stomacher Lab-blender (Model 400, Spiral b Samples exhibited evidence of spoilage (undesirable odor and appearance).
Table 3—Growth of background bacterial populations a as affected by treatments in vacuum-packaged pre-peeled potatoes stored at 28°C
Time (h)
Treatment 0 4 8 12 18 24 48
Aerobic Sulfite 4.22 ± 0.09 2.98 ± 0.11 3.65 ± 0.46 2.76 ± 0.28 3.77 ± 0.33 6.53 ± 0.19 8.37 ± 0.53b
Plate CBI 4.22 ± 0.09 2.86 ± 0.24 3.03 ± 0.37 5.38 ± 0.28 6.17 ± 0.19 8.54 ± 0.14b 9.03 ± 0.27b
Counts CBI-RINSE 4.22 ± 0.09 2.79 ± 0.35 3.32 ± 0.26 5.08 ± 0.42 5.32 ± 0.27 7.26 ± 0.21b 8.32 ± 0.16b
Control 4.22 ± 0.09 3.71 ± 0.26 4.29 ± 0.26 7.12 ± 0.05 7.80 ± 0.08 10.35 ± 0.19b 8.82 ± 0.42b
Anaerobic Sulfite 3.36 ± 0.15 5.20 ± 0.28 5.41 ± 0.39 4.28 ± 0.43 3.44 ± 0.32 4.33 ± 0.59 7.28 ± 0.31
Plate CBI 3.36 ± 0.15 5.28 ± 0.17 4.94 ± 0.32 4.24 ± 0.21 6.12 ± 0.37 9.67 ± 0.13 8.83 ± 0.29
Counts CBI-RINSE 3.36 ± 0.15 3.74 ± 0.11 3.34 ± 0.13 4.45 ± 0.28 5.35 ± 0.33 6.13 ± 0.28 8.32 ± 0.44
Control 3.36 ± 0.15 5.22 ± 0.27 4.84 ± 0.37 7.15 ± 0.35 8.45 ± 0.06 8.93 ± 0.19 8.81 ± 0.43
CVT Sulfite 3.59 ± 0.12 3.54 ± 0.04 3.96 ± 0.11 3.91 ± 0.32 3.82 ± 0.19 5.12 ± 0.51 7.12 ± 0.23
Plate CBI 3.59 ± 0.12 3.48 ± 0.24 3.97 ± 0.26 4.12 ± 0.17 6.44 ± 0.36 7.33 ± 0.07 9.34 ± 0.43
Counts CBI-RINSE 3.59 ± 0.12 4.19 ± 0.08 3.47 ± 0.08 4.97 ± 0.33 4.66 ± 0.08 6.96 ± 0.33 7.85 ± 0.34
Control 3.59 ± 0.12 3.95 ± 0.31 3.87 ± 0.17 6.64 ± 0.31 7.30 ± 0.50 8.59 ± 0.53 8.61 ± 0.41
a All plate counts are log
10 CFU/g and represent means ± standard deviations of two replications.
b Samples exhibited evidence of spoilage (undesirable odor and appearance).
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