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POSTED ON SEPTEMBER 30, 2015 BY DR. DEEPAK BHANOT 1 COMMENT

Advantages of HPLC over Thin-

layer Chromatography (TLC)
The advantages of GC over TLC
were discussed in an earlier
article. The characteristics that
make HPLC popular were also
discussed in the article Why is
HPLC the most popular
technique in analytical
laboratories today.

HPLC is the successor of TLC

and has made signi cant
contributions to the growth of
analytical science and its HPLC systems

diverse applications in
pharmaceuticals, foods,
polymers and plastics, environmental monitoring and clinical elds.

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A comparison on characteristic features of HPLC that make it score over TLC are discussed in the
present article TODAY WEEK MONTH ALL

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In TLC separations it becomes di cult to di erentiate between overlapping bands and spots. In
contrast the peaks in HPLC can be easily resolved and evaluated by controlling operational
parameters such as ow rate of mobile phase, bu er control of the mobile phase, column oven TOPICS
temperature, etc.
AAS (53)

Speed of analysis Analytical Chemistry (3)

In TLC separations the carrier liquid progression on the plate is dependent on gravitational force Calculations (5)
or capillary action. However, in HPLC the ow of mobile phase through the column is fast as it is Chemical (2)
pushed through with the help of a pump.Di erent pump pressure ranges can be achieved using
Chromatography (9)
normal as well as ultra high pressure systems
Environmental Analysis (2)

Quanti cation of results Featured (2)

HPLC software is capable of reporting precise and accurate results based on area counts of Free e-courses (2)
peaks. TLC quanti cations are based on visual comparisons or spot intensity matching
Gas Chromatography (55)
techniques which can be less quantitative in practice.
General Topics (140)

Sensitivity Guest Posts (27)

HPLC o ers a large number of detector options to choose from such as UV, refractive index, HPLC (60)
electrochemical, uorescence, conductivity, mass selectivity detector. Simultaneous detection is
ICP Techniques (13)
also possible using the photodiode array detection system. Such large and sensitive detection
options are not available in TLC separations. Jobs (1)

Lab Bytes (17)

Software
Lab Management (95)

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HPLC software is capable of reporting precise and accurate results based on area counts of Laboratory Insurance (8)
peaks. In addition software also helps set and monitor operational parameters such as ow rate,
Laboratory Safety (13)
detector wavelength, column temperature,etc.
Method Validation (1)

Choice of stationary phase and columns Microbiology (14)

A large number of stationary phases are available to suit di erent range of applications .Similarly News (3)
columns of di erent bores and lengths are available for micro level determinations to Paper Chromatography (10)
preparative scale preparations. This unique facility is not available on TLC plate separations.
pH Measurement (3)

Storage of results Preformulation Studies (3)

Like GC it is possible to keep a permanent record of generated chromatograms as hard or soft Quality System (9)
copies. TLC plates cannot be stored due to fading of spots over time and requirement of proper Separation Science (11)
storage conditions. However, pictures of TLC plates can be taken and stored as permanent
Spectroscopy (80)
records.
Stability studies (8)

Hyphenated techniques Training (21)

HPLC has been successfully coupled with mass spectrometers and FT-IR systems and this has Weighing (3)
resulted in signi cant improvement in both resolution and sensitivity. This option is not possible
in TLC separations.

Reuse of Columns
The columns if maintained properly and operated under carefully controlled conditions without
overloading can be used repeatedly for signi cant time periods. In contrast new TLC plates are
required for each analysis.

HPLC is undoubtedly a rapidly growing chromatography technique but like any other analytical
technique it has certain limitations which will be discussed subsequently in another article.

Filed Under: HPLC

About Dr. Deepak Bhanot

Dr Deepak Bhanot is a seasoned professional having nearly 30 years expertise
beginning from sales and product support of analytical instruments. After
completing his graduation and post graduation from Delhi University and IIT
Delhi he went on to Loughborough University of Technology, UK for doctorate research in
analytical chemistry. His mission is to develop training programs on analytical techniques and
share his experiences with broad spectrum of users ranging from professionals engaged in
analytical development and research as well as young enthusiasts fresh from academics who
wish to embark upon a career in analytical industry.

Comments

Bello Yusuf Yunusa says:

July 23, 2016 at 9:05 pm

An importance personality, wish all the best.

Reply

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