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Membuat Ringkasan Artikel internasional

Judul Artikel : An Amperometric Biosensor for the Determination of Bacterial Sepsis Biomarker, Secretory
Phospholipase Group 2-IIA Using a Tri-Enzyme System

Authors/penulis : Nik Nurhanan Nik Mansor, Tan Toh Leong, Eka Safitri, Dedi Futra, Nurul Saadah Ahmad,
Dian Nasriana Nasuruddin, Azlin Itnin, Ida Zarina Zaini, Khaizurin Tajul Arifin,
 Lee Yook
Heng and Nurul Izzaty Hassan

Nama Jurnal : Sensors

Volume/seri/halaman : 18 / 686 / 1-15
Tahun : 2018

Ringkasan desain biosensor

Jenis Biosensor Biosensor design Transduksi Prinsip /konsep reaksi biosensor Referensi
digunakan
Enzyme of choline Screen printed Amperometric The production of the enzymes PLA2-IIA during sepsis conditions Mansor et al
kinase/choline electrode can be reacted with the substrate, phosphatidylcholine (PC) to form 2018
oxidase/horseradish (SPE)/Gold phosphocholine. Choline kinase (also known as choline
peroxidase nanoparticles phosphokinase) is an enzyme which catalyses phosphocholine.
(AuNPs) Choline kinase (also known as choline phosphokinase) is an enzyme
/acrylic which the conversion of phosphocholine to choline. This is then
microspheres acted upon by the second enzyme choline catalyses the conversion
via succinimide of phosphocholine to choline. This is then acted upon by the second
groups enzyme oxidase (ChOx) to produce hydrogen peroxide. Further, the
third enzyme horseradish peroxidase choline oxidase (ChOx) to
produce hydrogen peroxide. Further, the third enzyme horseradish
(HRP) was used to react with hydrogen peroxide and this reaction is
 indicated by the redox mediator, peroxidase (HRP) was used to react
with hydrogen peroxide and this reaction is indicated by the
potassium ferricyanide K3Fe(CN)6 operating at lower potential

Ringkasan Prestasi biosensor yang dikembangkan

Jenis Biosensor Biosensor analit Sensitivitas Linear Limit of detection Response Shelf- life Referensi
Design range LOD time (day)
(menit)
Enzyme of choline SPE/ AuNPs bacterial 0.111 0.01 – 100 5 x 10-3 ng/mL 10 25 Mansor et al
kinase/choline / acrylic sepsis ng/mL 2018
oxidase/horseradish microspheres infection
peroxidase